Product: TNNI3 Antibody
Catalog: AF7807
Description: Rabbit polyclonal antibody to TNNI3
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 26kDa; 24kD(Calculated).
Uniprot: P48787
RRID: AB_2844171

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 50ul $250 In stock
 100ul $350 In stock
 200ul $450 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
TNNI3 Antibody detects endogenous levels of total TNNI3.
RRID:
AB_2844171
Cite Format: Affinity Biosciences Cat# AF7807, RRID:AB_2844171.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cardiac muscle; Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); Cardiomyopathy, familial hypertrophic, 7, included; CMD1FF; CMD2A; CMH7; cTnI; Familial hypertrophic cardiomyopathy 7; MGC116817; RCM1; Tn1; Tni; TNN I3; TNNC 1; TNNC1; TNNI3; TNNI3_HUMAN; Troponin I; Troponin I cardiac; Troponin I cardiac muscle; Troponin I cardiac muscle isoform; Troponin I type 3 cardiac; troponin I, cardiac 3; TroponinI; Ttroponin I type 3 (cardiac);

Immunogens

Immunogen:

A synthesized peptide derived from mouse TNNI3, corresponding to a region within N-terminal amino acids.

Uniprot:
Sequence:
MADESSDAAGEPQPAPAPVRRRSSANYRAYATEPHAKKKSKISASRKLQLKTLMLQIAKQEMEREAEERRGEKGRVLRTRCQPLELDGLGFEELQDLCRQLHARVDKVDEERYDVEAKVTKNITEIADLTQKIYDLRGKFKRPTLRRVRISADAMMQALLGTRAKESLDLRAHLKQVKKEDIEKENREVGDWRKNIDALSGMEGRKKKFEG

PTMs - P48787 As Substrate

Site PTM Type Enzyme
S5 Phosphorylation
S6 Phosphorylation
S23 Phosphorylation P68404-2 (Prkcb) , P16054 (Prkce) , P05132 (Prkaca) , P70336 (Rock2) , P20444 (Prkca) , P18653 (Rps6ka1)
S24 Phosphorylation P68404-2 (Prkcb) , P18653 (Rps6ka1) , P05132 (Prkaca) , P20444 (Prkca) , P70336 (Rock2) , P16054 (Prkce)
Y27 Phosphorylation
K37 Ubiquitination
S43 Phosphorylation P68404-2 (Prkcb) , P20444 (Prkca)
S45 Phosphorylation P20444 (Prkca) , P68404-2 (Prkcb)
K51 Ubiquitination
K59 Ubiquitination
C81 S-Nitrosylation
C98 S-Nitrosylation
K107 Ubiquitination
K118 Ubiquitination
K121 Ubiquitination
K132 Ubiquitination
Y134 Phosphorylation
T144 Phosphorylation P68404-2 (Prkcb) , P70336 (Rock2) , P20444 (Prkca)
S151 Phosphorylation
K165 Ubiquitination
S167 Phosphorylation
K175 Ubiquitination
K184 Ubiquitination
K194 Acetylation
K194 Ubiquitination
S200 Phosphorylation

Research Backgrounds

Function:

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

PTMs:

Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-32. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T) (By similarity). Phosphorylated at Ser-43 and Ser-45 by PRKCE; phosphorylation increases myocardium contractile dysfunction.

Subunit Structure:

Interacts with TRIM63 (By similarity). Binds to actin and tropomyosin. Interacts with STK4/MST1 (By similarity).

Family&Domains:

Belongs to the troponin I family.

Restrictive clause

 

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For Research Use Only.
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