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  • Product Name
    Phospho-JNK1/2/3 (Thr183+Tyr185) Antibody
  • Catalog No.
    AF3318
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity:
    Human, Mouse, Rat
  • Prediction:
    Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
  • UniProt
  • Mol.Wt.
    46,54kDa
  • Concentration
    1mg/ml
  • Browse similar products>>

Product Information

Alternative Names:Expand▼

C Jun kinase 2; c Jun N terminal kinase 1; c Jun N terminal kinase 2; c Jun N terminal kinase 3; c-Jun N-terminal kinase 1; JNK 46; JNK 55; JNK; JNK-46; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; JNK3 alpha protein kinase; JNK3; JNK3A; Jun kinase; JUN N terminal kinase; MAP kinase 10; MAP kinase 8; MAP kinase 9; MAP kinase p49 3F12; MAPK 10; MAPK 8; MAPK 9; MAPK10; mapk8; MAPK9; Mitogen activated protein kinase 10; Mitogen activated protein kinase 8; Mitogen activated protein kinase 8 isoform JNK1 alpha1; Mitogen activated protein kinase 8 isoform JNK1 beta2; Mitogen activated protein kinase 9; Mitogen-activated protein kinase 8; MK08_HUMAN; p493F12; p54a; p54aSAPK; p54bSAPK; PRKM10; PRKM8; PRKM9; SAPK; SAPK(beta); SAPK1; SAPK1a; SAPK1b; SAPK1c; Stress activated protein kinase 1; Stress activated protein kinase 1a; Stress activated protein kinase 1b; Stress activated protein kinase 1c; Stress activated protein kinase beta; Stress activated protein kinase JNK1; Stress activated protein kinase JNK2; Stress activated protein kinase JNK3; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1; c Jun kinase 2; C Jun N terminal kinase 2; c-Jun N-terminal kinase 2; JNK 55; JNK-55; JNK2 alpha; JNK2; JNK2 beta; JNK2A; JNK2alpha; JNK2B; JNK2BETA; Jun kinase; MAP kinase 9; MAPK 9; Mapk9; Mitogen activated protein kinase 9; Mitogen-activated protein kinase 9; MK09_HUMAN; P54a; p54aSAPK; PRKM9; Protein kinase, mitogen-activated, 9; SAPK alpha; SAPK; SAPK1a; Stress activated protein kinase 1a; Stress-activated protein kinase JNK2; c Jun kinase 3; c-Jun N-terminal kinase 3; cJun N terminal kinase 3; FLJ12099; FLJ33785; JNK3 alpha protein kinase; JNK3; JNK3A; MAP kinase 10; MAP kinase; MAP kinase p49 3F12; MAPK 10; Mapk10; MGC50974; mitogen activated protein kinase 10; Mitogen-activated protein kinase 10; MK10_HUMAN; p493F12; p54bSAPK; PRKM10; protein kinase mitogen activated 10; SAPK1b; Stress activated protein kinase 1b; stress activated protein kinase beta; Stress activated protein kinase JNK3; Stress-activated protein kinase JNK3;

Applications:

WB 1:500-1:2000, IHC 1:100-1:500, IF 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-JNK1/2/3 (Thr183+Tyr185) Antibody detects endogenous levels of JNK1/2/3 only when phosphorylated at Threonine 183+Tyrosine 185.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human JNK1/2/3 around the phosphorylation site of Thr183+Tyr185.

Uniprot:



>>Visit The Human Protein Atlas

Gene id:

Molecular Weight:

Observed Mol.Wt.: 46,54kDa.
Predicted Mol.Wt.: 49kDa.

Subcellular Location:

Cytoplasm. Nucleus.

Description:

JNK3 a protein kinase of the MAPK family that is potently activated by a variety of environmental stress and pro-inflammatory cytokines. Brain-selective JNK isoform.

Sequence:
        10         20         30         40         50
MSRSKRDNNF YSVEIGDSTF TVLKRYQNLK PIGSGAQGIV CAAYDAILER
60 70 80 90 100
NVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKSLEE
110 120 130 140 150
FQDVYIVMEL MDANLCQVIQ MELDHERMSY LLYQMLCGIK HLHSAGIIHR
160 170 180 190 200
DLKPSNIVVK SDCTLKILDF GLARTAGTSF MMTPYVVTRY YRAPEVILGM
210 220 230 240 250
GYKENVDLWS VGCIMGEMVC HKILFPGRDY IDQWNKVIEQ LGTPCPEFMK
260 270 280 290 300
KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK
310 320 330 340 350
MLVIDASKRI SVDEALQHPY INVWYDPSEA EAPPPKIPDK QLDEREHTIE
360 370 380 390 400
EWKELIYKEV MDLEERTKNG VIRGQPSPLG AAVINGSQHP SSSSSVNDVS
410 420
SMSTDPTLAS DTDSSLEAAA GPLGCCR

Background

Function:

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed:10747973).

Post-translational Modifications:

Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme (PubMed:11062067). Phosphorylated by TAOK2 (PubMed:17158878). May be phosphorylated at Thr-183 and Tyr-185 by MAP3K1/MEKK1 (PubMed:17761173).

Subcellular Location:

Nucleus;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4 (PubMed:15693750). These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX (PubMed:12514174). Interacts with JAMP (By similarity). Forms a complex with MAPK8IP1 and ARHGEF28 (By similarity). Interacts with HSF1 (via D domain and preferentially with hyperphosphorylated form); this interaction occurs under both normal growth conditions and immediately upon heat shock (PubMed:10747973). Interacts (phosphorylated form) with NFE2; the interaction phosphorylates NFE2 in undifferentiated cells (By similarity). Interacts with NFATC4 (PubMed:17875713). Interacts with MECOM; regulates JNK signaling (PubMed:10856240). Interacts with PIN1; this interaction mediates MAPK8 conformational changes leading to the binding of MAPK8 to its substrates (PubMed:21660049). Interacts with GRIPAP1 (PubMed:17761173).

Similarity:

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Research Fields

Research Fields:

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction.(View pathway)
· Cellular Processes > Cell growth and death > Apoptosis.(View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal.(View pathway)
· Cellular Processes > Cell growth and death > Apoptosis - multiple species.(View pathway)
· Cellular Processes > Cell growth and death > Necroptosis.(View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Wnt signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway.(View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.(View pathway)
· Human Diseases > Cancers: Specific types > Pancreatic cancer.(View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Colorectal cancer.(View pathway)
· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Human Diseases > Cancers: Overview > Choline metabolism in cancer.(View pathway)
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.(View pathway)
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.(View pathway)
· Organismal Systems > Immune system > Toll-like receptor signaling pathway.(View pathway)
· Organismal Systems > Immune system > IL-17 signaling pathway.(View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.(View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
· Organismal Systems > Endocrine system > Relaxin signaling pathway.
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
· Organismal Systems > Endocrine system > Insulin signaling pathway.(View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation.(View pathway)
· Organismal Systems > Endocrine system > Prolactin signaling pathway.(View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway.(View pathway)
· Organismal Systems > Nervous system > Dopaminergic synapse.
· Organismal Systems > Development > Osteoclast differentiation.(View pathway)
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.(View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway.(View pathway)
· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.(View pathway)

Western blot analysis of Phospho-JNK1/2/3 (Thr183+Tyr185) using various lysates Lanes 1 - 2: Merged signal (red and green). Green - AF3318 observed at 46,54 kDa. Red - loading control, T0023, observed at 55 kDa. Blots were developed with Goat Anti-Rabbit IgG(H+L) FITC–conjugated (S0008) and Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated (S0005) secondary antibodies
Western blot analysis of extracts from mouse brain/rat heart, using Phospho-JNK1/2/3 (Thr183+Tyr185) Antibody. -/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide)
AF3318 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF3318 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF3318 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF3318 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF3318 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF3318 at 1/200 staining Mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF3318 at 1/200 staining Mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF3318 at 1/200 staining Mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF3318 at 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF3318 at 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF3318 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF3318 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF3318 at 1/200 staining Human bladder cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF3318 at 1/200 staining Human bladder cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF3318 at 1/200 staining Human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF3318 at 1/200 staining Human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF3318 at 1/200 staining Human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Phospho-JNK1/2/3 (Thr183+Tyr185) Antibody for IHC in human brain tissue.
AF3318 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
AF3318 staining HeLa  cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.
ELISA analysis of AF3318 showing specificity to Phospho-JNK1/2/3 (Thr183+Tyr185) peptide. Peptides concentration: 1ug/ml.
P-peptide: phospho-peptide; N-peptide: non-phospho-peptide.

Reference Citations:

1). Zhang J et al. S100A16 suppresses the growth and survival of leukaemia cells and correlates with relapse and relapse free survival in adults with Philadelphia chromosome-negative B-cell acute lymphoblastic leukaemia. Br J Haematol 2019 Mar 27 (PubMed: 30916375) [IF=5.206]

2). Fan H et al. The in vitro and in vivo anti-inflammatory effect of osthole, the major natural coumarin from Cnidium monnieri (L.) Cuss, via the blocking of the activation of the NF-κB and MAPK/p38 pathways. Phytomedicine 2019 Feb 18;58:152864 (PubMed: 30878874) [IF=4.180]

3). Geng J et al. Trimethylamine N-oxide promotes atherosclerosis via CD36-dependent MAPK/JNK pathway. Biomed Pharmacother 2018 Jan;97:941-947 (PubMed: 29136772) [IF=3.743]

4). Yang Y et al. Black Sesame Seeds Ethanol Extract Ameliorates Hepatic Lipid Accumulation, Oxidative Stress and Insulin Resistance in Fructose-induced Nonalcoholic Fatty Liver Disease. J Agric Food Chem 2018 Sep 24 (PubMed: 30244573) [IF=3.571]

5). Tan L et al. Dihydroberberine, a hydrogenated derivative of berberine firstly identified in Phellodendri Chinese Cortex, exerts anti-inflammatory effect via dual modulation of NF-κB and MAPK signaling pathways. Int Immunopharmacol 2019 Aug 8;75:105802 (PubMed: 31401380) [IF=3.361]

6). Tang Q et al. Ferroptosis is newly characterized form of neuronal cell death in response to arsenite exposure. Neurotoxicology 2018 Jul;67:27-36 (PubMed: 29678591) [IF=3.311]

7). Li S et al. Effect of CAPE-pNO2 against type 2 diabetes mellitus via the AMPK/GLUT4/ GSK3β/PPARα pathway in HFD/STZ-induced diabetic mice. Eur J Pharmacol 2019 Mar 15;853:1-10 (PubMed: 30885574) [IF=3.170]

8). Liu Y et al. (3R, 7R)-7-Acetoxyl-9-Oxo-de-O-Methyllasiodiplodin, a Secondary Metabolite of Penicillium Sp., Inhibits LPS-Mediated Inflammation in RAW 264.7 Macrophages through Blocking ERK/MAPKs and NF-κB Signaling Pathways. Inflammation 2019 Apr 23 (PubMed: 31011928)

9). Ji M et al. The p75 neurotrophin receptor might mediate sepsis-induced synaptic and cognitive impairments. Behav Brain Res 2018 Jul 16;347:339-349 (PubMed: 29604364)

10). Zhang BB et al. Neuroprotective Effects of Dammarane-Type Saponins from Panax notoginseng on Glutamate-Induced Cell Damage in PC12 Cells. Planta Med 2019 Feb 21 (PubMed: 30791058)

11). Wang R et al. PM2.5 upregulates rat mesenteric arteries 5-HT2A receptor via inflammatory-mediated mitogen-activated protein kinases signaling pathway. Environ Toxicol 2019 Jun 14 (PubMed: 31199065)

12). An S et al. Administration of CoCl2 Improves Functional Recovery in a Rat Model of Sciatic Nerve Transection Injury. Int J Med Sci 2018 Sep 7;15(13):1423-1432 (PubMed: 30443161)

13). Zhang T et al. Interaction with tumor‑associated macrophages promotes PRL‑3‑induced invasion of colorectal cancer cells via MAPK pathway‑induced EMT and NF‑κB signaling‑induced angiogenesis. Oncol Rep 2019 Mar 7 (PubMed: 30864736)

14). Geng H et al. Cigarette smoke extract-induced proliferation of normal human urothelial cells via the MAPK/AP-1 pathway. Oncol Lett 2017 Jan;13(1):469-475 (PubMed: 28123584)

Application: WB    Species:human;    Sample:SV-HUC-1 cells

Figure 4. (A) Fold change in cell viability of SV-HUC-1 cells treated with 0, 0.10 and 0.25% CSE for 7 days in combination with 5 µM U0126, 5 µM SB203580 or 2 µM SP600125. Data are representative of three independent experiments and are expressed as the mean ± standard deviation. * P


15). Zhao L et al. MAPK/AP-1 pathway regulates benzidine-induced cell proliferation through the control of cell cycle in human normal bladder epithelial cells. Oncol Lett 2018 Oct;16(4):4628-4634 (PubMed: 30197677)

16). Li H et al. Dysifragilone A inhibits LPS‑induced RAW264.7 macrophage activation by blocking the p38 MAPK signaling pathway. Mol Med Rep 2018 Jan;17(1):674-682 (PubMed: 29115475)

17). Jin X et al. 11-O-acetylcyathatriol inhibits MAPK/p38-mediated inflammation in LPS-activated RAW 264.7 macrophages and has a protective effect on ethanol-induced gastric injury. Mol Med Rep 2016 Jul;14(1):874-80 (PubMed: 27222252)

Application: WB    Species:mouse;    Sample:Not available

Figure 3. Effects of 11‑O‑acetylcyathatriol on the protein expression levels of iNOS and COX‑2. The RAW 264.7 cells were treated by 1 µg/ml of LPS with indicated concentrations of 11-O‑acetylcyathatriol (12.5, 25, 50 and 100 µM) for 24 h, and the expression levels of (A) iNOS and COX‑2 were detected using western blot analysis. (B) Effects of 11‑O-acetylcyathatriol on the phosphorylation of ERK1/2, JNK and p38 proteins. RAW 264.7 cells were treated with 1 µg/ml LPS with 11‑O‑acetylcyathatriol (12.5, 25, 50 and 100 µM) for 30 min, and the protein expression levels of p‑ERK1/2, p‑JNK and p‑p38 were detected using western blot analysis. (C) Effects of 11‑O-acetylcyathatriol on the protein degradation of IκB-α. RAW 264.7 cells were treated with 1 µg/ml of LPS with 11-O‑acetylcyathatriol (12.5, 25, 50 and 100 µM) for 10 min, and the protein expression of IκB-α was detected using western blot analysis. iNOS, inducible nitric oxide synthase; COX‑2, cyclooxygenase‑2; LPS, lipopolysaccharide; ERK, extracellular signal‑regulated kinase; JNK, c‑Jun N‑terminal kinase; IκB-α, inhibitor of nuclear factor-κB-α; p‑, phosphorylated.


18). Zhang X et al. Neuroprotective Effect of Modified Xijiao Dihuang Decoction against Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury in PC12 Cells: Involvement of TLR4-MyD88/NF-κB Signaling Pathway. Evid Based Complement Alternat Med 2017;2017:3848595 (PubMed: 29234386)

19). Jin X et al. Tiliroside, the major component of Agrimonia pilosa Ledeb ethanol extract, inhibits MAPK/JNK/p38-mediated inflammation in lipopolysaccharide-activated RAW 264.7 macrophages. Exp Ther Med 2016 Jul;12(1):499-505 (PubMed: 27347085)

Application: WB    Species:mouse;    Sample:Not available

Figure 7. Effect of tiliroside on the phosphorylation of mitogen‑activated protein kinase‑ERK/JNK/p38 proteins. (A) RAW 264.7 cells were treated with LPS 1 µg/ml with or without tiliroside (12.5, 25, 50, and 100 µM) for 45 min and the expression of p‑ERK1/2, p‑JNK and p‑p38 was assessed by western blot analysis. Detection of β‑actin was conducted to confirm the equal loading of proteins. Densitometric analysis of p‑ERK1/2 (B) p‑JNK (C) and p‑p38 (D) expression represent the mean±standard deviation of three separate experiments. Data were normalized with respect to β‑actin levels. **P


20). Zhang Q et al. Anti-inflammatory action of ambuic acid, a natural product isolated from the solid culture of Pestalotiopsis neglecta, through blocking ERK/JNK mitogen-activated protein kinase signaling pathway. Exp Ther Med 2018 Aug;16(2):1538-1546 (PubMed: 30116402)

21). Peng J et al. MiR-377 promotes white adipose tissue inflammation and decreases insulin sensitivity in obesity via suppression of sirtuin-1 (SIRT1). Oncotarget 2017 Jul 31;8(41):70550-70563 (PubMed: 29050301)

Application: WB    Species:mouse;    Sample:Not available

Figure 4: MiR-377 promotes inflammation and insulin-resistance in mature 3T3-L1 cells. After transfection with 100 nM miR-377 mimics or inhibitor for 24 h, differentiated 3T3-L1 adipocytes were treated with 10 ng/ml TNFα for 24 h and then stimulated with 100 nM insulin for 15 min. Cells were then harvested for real-time PCR and immunoblotting analyses. (A) The effect of miR-377 overexpression on inflammation-related gene expression. *P < 0.05, **P < 0.01 vs. NC 0.1% BSA; ##P < 0.01 vs. NC 10 ng/ml TNFα; ns, not signifcant (n = 3). (B) The effect of miR-377 inhibition on inflammation-related gene expression under conditions of TNFα-induced insulin-resistance. ##P < 0.01 vs. NC 10 ng/ml TNFα(n = 3). (C) The effect of miR-377 overexpression on JNK phosphorylation under conditions of TNFα-induced insulin-resistance. *P < 0.05 vs. NC without insulin; #P < 0.05 vs. NC with insulin (n = 3). (D and E) The effect of miR-377 overexpression on AKT and ERK phosphorylation. *P < 0.05 vs. NC with 0.1% BSA and insulin; #P < 0.05 vs. NC with 10 ng/ml TNFα and insulin (n = 3). (F) The effect of miR-377 inhibition on JNK phosphorylation under conditions of TNFα-induced insulin-resistance. *P < 0.05 vs. NC without insulin; #P < 0.05 vs. NC with insulin (n = 3). (G and H) The effect of miR-377 inhibition on AKT and ERK phosphorylation. *P < 0.05 vs. NC with 0.1% BSA and insulin; #P < 0.05 vs. NC with 10 ng/ml TNFα and insulin (n = 3).


22). Yang B et al. Mucin 17 inhibits the progression of human gastric cancer by limiting inflammatory responses through a MYH9-p53-RhoA regulatory feedback loop. J Exp Clin Cancer Res 2019 Jul 1;38(1):283 (PubMed: 31262330)

23). Li Zhao et al. MAPK/AP‑1 pathway regulates benzidine‑induced cell proliferation through the control of cell cycle in human normal bladder epithelial cells. ONCOL LETT 2018;16(4):4628-4634

24). Meng Zhou et al. Administration of CoCl 2 Improves Functional Recovery in a Rat Model of Sciatic Nerve Transection Injury. Int J Med Sci 2018; 15(13):1423-1432

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Catalog Number :

AF3318-BP
(Blocking peptide available as AF3318-BP)

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Xenopus
100%
Chicken
100%
Rabbit
100%
Pig
100%
Dog
100%
Bovine
100%
Sheep
100%
Horse
100%
Zebrafish
79%
High similarity Medium similarity Low similarity No similarity
P45983/P45984/P53779 as Substrate
Site PTM Type Enzyme
S2 Phosphorylation
K30 Ubiquitination
K56 Ubiquitination
K68 Ubiquitination
C116 S-Nitrosylation
S129 Phosphorylation Q05655 (PRKCD)
S144 Phosphorylation
K153 Ubiquitination
S155 Phosphorylation
K160 Ubiquitination
K166 Ubiquitination
T178 Phosphorylation
S179 Phosphorylation
T183 Phosphorylation P45985 (MAP2K4) , O14733 (MAP2K7) , Q99683 (MAP3K5) , O95382 (MAP3K6)
Y185 Phosphorylation P45985 (MAP2K4) , P07949 (RET) , Q99683 (MAP3K5) , O14733 (MAP2K7) , O95382 (MAP3K6)
T188 Phosphorylation
T243 Phosphorylation
K250 Ubiquitination
K251 Ubiquitination
T255 Phosphorylation
T258 Phosphorylation
Y259 Phosphorylation
K265 Ubiquitination
K273 Ubiquitination
K288 Ubiquitination
S292 Phosphorylation
K300 Ubiquitination
S307 Phosphorylation
K308 Acetylation
K308 Ubiquitination
K336 Ubiquitination
K353 Ubiquitination
Y357 Phosphorylation
T367 Phosphorylation
S377 Phosphorylation
Site PTM Type Enzyme
K56 Ubiquitination
K68 Ubiquitination
S144 Phosphorylation
K153 Ubiquitination
S155 Phosphorylation
K160 Ubiquitination
C163 S-Nitrosylation
K166 Ubiquitination
T178 Phosphorylation
T183 Phosphorylation O14733 (MAP2K7)
Y185 Phosphorylation P45985 (MAP2K4) , P07949 (RET) , O14733 (MAP2K7)
T188 Phosphorylation
K250 Acetylation
K250 Ubiquitination
K251 Ubiquitination
S292 Phosphorylation
K300 Ubiquitination
S311 Phosphorylation
K353 Ubiquitination
Y357 Phosphorylation
T386 Phosphorylation
T404 Phosphorylation O14733 (MAP2K7) , P68400 (CSNK2A1)
S407 Phosphorylation P68400 (CSNK2A1) , O14733 (MAP2K7)
Site PTM Type Enzyme
K94 Ubiquitination
K106 Ubiquitination
T131 Phosphorylation Q00535 (CDK5)
S182 Phosphorylation
K191 Ubiquitination
S193 Phosphorylation
K198 Ubiquitination
K204 Ubiquitination
T216 Phosphorylation
S217 Phosphorylation
T221 Phosphorylation O14733 (MAP2K7)
Y223 Phosphorylation P45985 (MAP2K4)
T226 Phosphorylation
T281 Phosphorylation
K288 Ubiquitination
K289 Ubiquitination
S330 Phosphorylation
K346 Acetylation
K391 Ubiquitination
P45983/P45984/P53779 as PTM Enzyme
Substrate Site Source
O00418 (EEF2K) S396 Uniprot
O43521-2 (BCL2L11) S44 Uniprot
O43521-17 (BCL2L11) T56 Uniprot
O43521-2 (BCL2L11) S58 Uniprot
O43521-1 (BCL2L11) S59 Uniprot
O43521-1 (BCL2L11) S69 Uniprot
O43521-1 (BCL2L11) S77 Uniprot
O43521-1 (BCL2L11) S104 Uniprot
O43521 (BCL2L11) T116 Uniprot
O43521-1 (BCL2L11) S118 Uniprot
O43524 (FOXO3) S574 Uniprot
O43561-2 (LAT) T155 Uniprot
O43561 (LAT) T184 Uniprot
O95644 (NFATC1) S172 Uniprot
P01106 (MYC) T58 Uniprot
P01106 (MYC) S62 Uniprot
P01106 (MYC) S71 Uniprot
P01106-2 (MYC) S77 Uniprot
P01106-2 (MYC) S86 Uniprot
P04150 (NR3C1) S226 Uniprot
P04637 (TP53) S20 Uniprot
P04637 (TP53) S37 Uniprot
P04637-1 (TP53) T81 Uniprot
P05067-4 (APP) T668 Uniprot
P05067 (APP) T743 Uniprot
P05412 (JUN) S63 Uniprot
P05412 (JUN) S73 Uniprot
P05412 (JUN) T91 Uniprot
P05412 (JUN) T93 Uniprot
P05787 (KRT8) S74 Uniprot
P05787 (KRT8) S432 Uniprot
P08047 (SP1) T278 Uniprot
P08047 (SP1) T739 Uniprot
P10275 (AR) S651 Uniprot
P10276 (RARA) T181 Uniprot
P10276 (RARA) S445 Uniprot
P10276 (RARA) S461 Uniprot
P10415-1 (BCL2) T69 Uniprot
P10415 (BCL2) S70 Uniprot
P10415 (BCL2) S87 Uniprot
P10636-8 (MAPT) T181 Uniprot
P10636-8 (MAPT) S202 Uniprot
P10636-8 (MAPT) T231 Uniprot
P10636-8 (MAPT) S396 Uniprot
P10636 (MAPT) T498 Uniprot
P10636 (MAPT) S516 Uniprot
P10636 (MAPT) S519 Uniprot
P10636 (MAPT) T522 Uniprot
P10636 (MAPT) T529 Uniprot
P10636 (MAPT) T534 Uniprot
P10636 (MAPT) S713 Uniprot
P10636 (MAPT) S721 Uniprot
P10636 (MAPT) S739 Uniprot
P14618-2 (PKM) T365 Uniprot
P15336 (ATF2) T69 Uniprot
P15336 (ATF2) T71 Uniprot
P15336 (ATF2) S90 Uniprot
P16104 (H2AFX) S140 Uniprot
P16949 (STMN1) S25 Uniprot
P16949 (STMN1) S38 Uniprot
P17275 (JUNB) T102 Uniprot
P17275 (JUNB) T104 Uniprot
P17535 (JUND) S90 Uniprot
P17535 (JUND) S100 Uniprot
P17535 (JUND) T117 Uniprot
P19419 (ELK1) S383 Uniprot
P19419 (ELK1) S389 Uniprot
P19793 (RXRA) S56 Uniprot
P19793 (RXRA) S70 Uniprot
P19793 (RXRA) T82 Uniprot
P19793 (RXRA) S260 Uniprot
P22736 (NR4A1) S95 Uniprot
P23443 (RPS6KB1) S434 Uniprot
P29353 (SHC1) S36 Uniprot
P30305-2 (CDC25B) S101 Uniprot
P30305-2 (CDC25B) S103 Uniprot
P30305 (CDC25B) S115 Uniprot
P30305 (CDC25B) S117 Uniprot
P30307 (CDC25C) S168 Uniprot
P31946 (YWHAB) S186 Uniprot
P31947 (SFN) S186 Uniprot
P35222 (CTNNB1) S33 Uniprot
P35222 (CTNNB1) S37 Uniprot
P35222 (CTNNB1) T41 Uniprot
P35568 (IRS1) S307 Uniprot
P35568 (IRS1) S312 Uniprot
P35568 (IRS1) S315 Uniprot
P35568 (IRS1) S616 Uniprot
P35568 (IRS1) S636 Uniprot
P36956-3 (SREBF1) T81 Uniprot
P36956-3 (SREBF1) S93 Uniprot
P36956 (SREBF1) S117 Uniprot
P37231-2 (PPARG) S84 Uniprot
P37231 (PPARG) S112 Uniprot
P38936 (CDKN1A) T57 Uniprot
P38936 (CDKN1A) S98 Uniprot
P38936 (CDKN1A) S130 Uniprot
P40763-2 (STAT3) S726 Uniprot
P40763 (STAT3) S727 Uniprot
P41970 (ELK3) S357 Uniprot
P41970 (ELK3) S363 Uniprot
P41970 (ELK3) S396 Uniprot
P41970 (ELK3) S401 Uniprot
P42224 (STAT1) S727 Uniprot
P42226 (STAT6) S707 Uniprot
P49023 (PXN) S178 Uniprot
P54259 (ATN1) S739 Uniprot
P55957 (BID) T59 Uniprot
P61978 (HNRNPK) S216 Uniprot
P61978 (HNRNPK) S353 Uniprot
P63104-1 (YWHAZ) S184 Uniprot
P68431 (HIST1H3J) S29 Uniprot
P78352 (DLG4) S295 Uniprot
P84243 (H3F3B) S29 Uniprot
P98177 (FOXO4) T227 Uniprot
P98177 (FOXO4) S230 Uniprot
P98177 (FOXO4) T451 Uniprot
P98177 (FOXO4) T455 Uniprot
Q00613-1 (HSF1) S363 Uniprot
Q01844 (EWSR1) T79 Uniprot
Q05639 (EEF1A2) S205 Uniprot
Q05639 (EEF1A2) S358 Uniprot
Q06481 (APLP2) T736 Uniprot
Q07817 (BCL2L1) T47 Uniprot
Q07817 (BCL2L1) S62 Uniprot
Q07817 (BCL2L1) T115 Uniprot
Q07820 (MCL1) S64 Uniprot
Q07820 (MCL1) S121 Uniprot
Q07820 (MCL1) T163 Uniprot
Q12904 (AIMP1) S140 Uniprot
Q12904 (AIMP1) T164 Uniprot
Q13043 (STK4) S82 Uniprot
Q13363 (CTBP1) S422 Uniprot
Q13950 (RUNX2) S118 Uniprot
Q14653 (IRF3) S173 Uniprot
Q14934 (NFATC4) S213 Uniprot
Q14934 (NFATC4) S217 Uniprot
Q15672 (TWIST1) S68 Uniprot
Q16621 (NFE2) S157 Uniprot
Q5JR12 (PPM1J) S93 Uniprot
Q5JR12 (PPM1J) T201 Uniprot
Q5JR12 (PPM1J) T204 Uniprot
Q8N122 (RPTOR) S696 Uniprot
Q8N122 (RPTOR) T706 Uniprot
Q8N122 (RPTOR) S863 Uniprot
Q8WYK2-1 (JDP2) T148 Uniprot
Q92934 (BAD) S75 Uniprot
Q92934 (BAD) S91 Uniprot
Q93045 (STMN2) S62 Uniprot
Q93045 (STMN2) S73 Uniprot
Q96EB6 (SIRT1) S27 Uniprot
Q96EB6 (SIRT1) S47 Uniprot
Q96EB6 (SIRT1) T530 Uniprot
Q96J02 (ITCH) S240 Uniprot
Q96J02 (ITCH) T263 Uniprot
Q96J02 (ITCH) S273 Uniprot
Q96LC9-2 (BMF) S74 Uniprot
Q96LC9-2 (BMF) S77 Uniprot
Q99607 (ELF4) S641 Uniprot
Q99640 (PKMYT1) S83 Uniprot
Q9H211 (CDT1) T29 Uniprot
Q9H2B2 (SYT4) S135 Uniprot
Q9NPI6 (DCP1A) S315 Uniprot
Q9NR28 (DIABLO) S6 Uniprot
Q9NR28 (DIABLO) S9 Uniprot
Q9NRA8 (EIF4ENIF1) S301 Uniprot
Q9NRA8 (EIF4ENIF1) S374 Uniprot
Q9NRA8 (EIF4ENIF1) S513 Uniprot
Q9NRA8 (EIF4ENIF1) S587 Uniprot
Q9NRA8 (EIF4ENIF1) S693 Uniprot
Q9NRA8 (EIF4ENIF1) S752 Uniprot
Q9UPT6-1 (MAPK8IP3) T265 Uniprot
Q9UPT6-1 (MAPK8IP3) T275 Uniprot
Q9UPT6 (MAPK8IP3) T286 Uniprot
Q9UQF2 (MAPK8IP1) S15 Uniprot
Q9UQF2 (MAPK8IP1) S29 Uniprot
Q9UQF2 (MAPK8IP1) T103 Uniprot
Q9UQF2 (MAPK8IP1) S197 Uniprot
Q9UQF2 (MAPK8IP1) T205 Uniprot
Q9UQF2 (MAPK8IP1) T284 Uniprot
Q9UQF2 (MAPK8IP1) S341 Uniprot
Q9UQF2 (MAPK8IP1) S421 Uniprot
Q9Y4H2 (IRS2) T350 Uniprot
Q9Y4H2 (IRS2) S491 Uniprot
Q9Y5Q3 (MAFB) T62 Uniprot
Q9Y6Q9 (NCOA3) T24 Uniprot
Q9Y6Q9 (NCOA3) S505 Uniprot
Q9Y6Q9 (NCOA3) S543 Uniprot
Q9Y6Q9 (NCOA3) S860 Uniprot
Q9Y6Q9 (NCOA3) S867 Uniprot
Substrate Site Source
O00418 (EEF2K) S396 Uniprot
O43521-2 (BCL2L11) T56 Uniprot
O43521-1 (BCL2L11) S59 Uniprot
O43521 (BCL2L11) S69 Uniprot
O43521-1 (BCL2L11) S77 Uniprot
O43602-2 (DCX) T321 Uniprot
O43602-2 (DCX) T331 Uniprot
O43602-2 (DCX) S334 Uniprot
O60239-2 (SH3BP5) S194 Uniprot
O60239-2 (SH3BP5) S264 Uniprot
O60239 (SH3BP5) S351 Uniprot
O60239-1 (SH3BP5) S421 Uniprot
O95140 (MFN2) S27 Uniprot
P01106 (MYC) S62 Uniprot
P01106 (MYC) S71 Uniprot
P04150 (NR3C1) S226 Uniprot
P04637 (TP53) S6 Uniprot
P04637 (TP53) S20 Uniprot
P04637 (TP53) S37 Uniprot
P04637 (TP53) T81 Uniprot
P05067-4 (APP) T668 Uniprot
P05067 (APP) T743 Uniprot
P05412 (JUN) S63 Uniprot
P05412 (JUN) S73 Uniprot
P10636-8 (MAPT) T181 Uniprot
P10636-8 (MAPT) S202 Uniprot
P10636-8 (MAPT) T212 Uniprot
P10636-8 (MAPT) T217 Uniprot
P10636-8 (MAPT) T231 Uniprot
P10636 (MAPT) T498 Uniprot
P10636 (MAPT) S516 Uniprot
P10636 (MAPT) S519 Uniprot
P10636 (MAPT) T529 Uniprot
P10636 (MAPT) T548 Uniprot
P10636 (MAPT) S713 Uniprot
P10636 (MAPT) S721 Uniprot
P10636 (MAPT) S739 Uniprot
P15036 (ETS2) T72 Uniprot
P15336 (ATF2) T69 Uniprot
P15336 (ATF2) T71 Uniprot
P15336 (ATF2) S90 Uniprot
P16104 (H2AFX) S140 Uniprot
P16949 (STMN1) S25 Uniprot
P16949 (STMN1) S38 Uniprot
P19419-1 (ELK1) S383 Uniprot
P19419 (ELK1) S389 Uniprot
P19793 (RXRA) S56 Uniprot
P19793 (RXRA) S70 Uniprot
P19793 (RXRA) T82 Uniprot
P19793 (RXRA) S260 Uniprot
P23443 (RPS6KB1) S434 Uniprot
P29353 (SHC1) S36 Uniprot
P30307 (CDC25C) S168 Uniprot
P31645 (SLC6A4) T616 Uniprot
P31947 (SFN) S186 Uniprot
P35222 (CTNNB1) S191 Uniprot
P35222 (CTNNB1) S605 Uniprot
P35568 (IRS1) S307 Uniprot
P35568 (IRS1) S315 Uniprot
P35568 (IRS1) Y612 Uniprot
P35568 (IRS1) Y632 Uniprot
P40763 (STAT3) S727 Uniprot
P41970 (ELK3) S357 Uniprot
P41970 (ELK3) S363 Uniprot
P41970 (ELK3) S396 Uniprot
P41970 (ELK3) S401 Uniprot
P42224 (STAT1) S727 Uniprot
P46937 (YAP1) T119 Uniprot
P46937 (YAP1) S138 Uniprot
P46937 (YAP1) T154 Uniprot
P46937 (YAP1) S367 Uniprot
P49768 (PSEN1) S319 Uniprot
P49768 (PSEN1) T320 Uniprot
P50616 (TOB1) S152 Uniprot
P50616 (TOB1) S154 Uniprot
P50616 (TOB1) S164 Uniprot
P52945 (PDX1) S61 Uniprot
P52945 (PDX1) S66 Uniprot
P54259 (ATN1) S739 Uniprot
P55957 (BID) T59 Uniprot
P63104 (YWHAZ) S184 Uniprot
P68431 (HIST1H3J) S29 Uniprot
P84243 (H3F3B) S29 Uniprot
P98177 (FOXO4) T451 Uniprot
P98177 (FOXO4) T455 Uniprot
Q06481 (APLP2) T736 Uniprot
Q07817 (BCL2L1) T47 Uniprot
Q07817 (BCL2L1) S62 Uniprot
Q07817 (BCL2L1) T115 Uniprot
Q12968 (NFATC3) S163 Uniprot
Q12968-1 (NFATC3) S165 Uniprot
Q14653 (IRF3) S173 Uniprot
Q14934 (NFATC4) S213 Uniprot
Q14934 (NFATC4) S217 Uniprot
Q5JR12 (PPM1J) S93 Uniprot
Q8IW41 (MAPKAPK5) T182 Uniprot
Q8WYK2 (JDP2) T148 Uniprot
Q9BQQ3 (GORASP1) S274 Uniprot
Q9NYV6 (RRN3) T200 Uniprot
Q9UIG0 (BAZ1B) S158 Uniprot
Q9UPT6 (MAPK8IP3) T265 Uniprot
Q9UPT6 (MAPK8IP3) T275 Uniprot
Q9UPT6 (MAPK8IP3) T286 Uniprot
Q9UQF2 (MAPK8IP1) T103 Uniprot
Substrate Site Source
O43521-2 (BCL2L11) S58 Uniprot
O43521-1 (BCL2L11) S59 Uniprot
O43521-1 (BCL2L11) S69 Uniprot
O43521-1 (BCL2L11) S77 Uniprot
O43521 (BCL2L11) T116 Uniprot
O60282 (KIF5C) S176 Uniprot
O95644 (NFATC1) S172 Uniprot
P01106 (MYC) T58 Uniprot
P01106 (MYC) S62 Uniprot
P01106 (MYC) S71 Uniprot
P04150 (NR3C1) S226 Uniprot
P04637 (TP53) S37 Uniprot
P05067-4 (APP) T668 Uniprot
P05067 (APP) T743 Uniprot
P05412 (JUN) S63 Uniprot
P05412 (JUN) S73 Uniprot
P10415 (BCL2) T56 Uniprot
P10415 (BCL2) S70 Uniprot
P10415 (BCL2) T74 Uniprot
P10415 (BCL2) S87 Uniprot
P10636 (MAPT) T498 Uniprot
P10636 (MAPT) S516 Uniprot
P10636 (MAPT) S519 Uniprot
P10636 (MAPT) T522 Uniprot
P10636 (MAPT) T529 Uniprot
P10636 (MAPT) T534 Uniprot
P10636 (MAPT) S713 Uniprot
P10636 (MAPT) S721 Uniprot
P10636 (MAPT) S739 Uniprot
P16949 (STMN1) S25 Uniprot
P16949 (STMN1) S38 Uniprot
P29353 (SHC1) S36 Uniprot
P30307 (CDC25C) S168 Uniprot
P31947 (SFN) S186 Uniprot
P54259 (ATN1) S739 Uniprot
P55957 (BID) T59 Uniprot
P61978-1 (HNRNPK) S216 Uniprot
P61978-1 (HNRNPK) S284 Uniprot
P61978-1 (HNRNPK) S353 Uniprot
P63104 (YWHAZ) S184 Uniprot
Q06481 (APLP2) T736 Uniprot
Q07820 (MCL1) S121 Uniprot
Q07820 (MCL1) T163 Uniprot
Q16600 (ZNF239) S38 Uniprot
Q16600 (ZNF239) S129 Uniprot
Q5JR12 (PPM1J) S93 Uniprot
Q93045 (STMN2) S62 Uniprot
Q93045 (STMN2) S73 Uniprot
Q9UPT6 (MAPK8IP3) T265 Uniprot
Q9UPT6 (MAPK8IP3) T275 Uniprot
Q9UPT6-1 (MAPK8IP3) T286 Uniprot
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.