Product: RanBP3 Antibody
Catalog: AF4795
Description: Rabbit polyclonal antibody to RanBP3
Application: WB
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 70kDa; 60kD(Calculated).
Uniprot: Q9H6Z4
RRID: AB_2844611

View similar products>>

   Size Price Inventory
 50ul $250 In stock
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(100%), Chicken(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
RanBP3 Antibody detects endogenous levels of total RanBP3.
RRID:
AB_2844611
Cite Format: Affinity Biosciences Cat# AF4795, RRID:AB_2844611.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp586I1520; Ran binding protein 3; Ran-binding protein 3; RANB3_HUMAN; RanBP3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9H6Z4 RANB3_HUMAN:

Widely expressed with high levels in testis and heart.

Sequence:
MADLANEEKPAIAPPVFVFQKDKGQKSPAEQKNLSDSGEEPRGEAEAPHHGTGHPESAGEHALEPPAPAGASASTPPPPAPEAQLPPFPRELAGRSAGGSSPEGGEDSDREDGNYCPPVKRERTSSLTQFPPSQSEERSSGFRLKPPTLIHGQAPSAGLPSQKPKEQQRSVLRPAVLQAPQPKALSQTVPSSGTNGVSLPADCTGAVPAASPDTAAWRSPSEAADEVCALEEKEPQKNESSNASEEEACEKKDPATQQAFVFGQNLRDRVKLINESVDEADMENAGHPSADTPTATNYFLQYISSSLENSTNSADASSNKFVFGQNMSERVLSPPKLNEVSSDANRENAAAESGSESSSQEATPEKESLAESAAAYTKATARKCLLEKVEVITGEEAESNVLQMQCKLFVFDKTSQSWVERGRGLLRLNDMASTDDGTLQSRLVMRTQGSLRLILNTKLWAQMQIDKASEKSIRITAMDTEDQGVKVFLISASSKDTGQLYAALHHRILALRSRVEQEQEAKMPAPEPGAAPSNEEDDSDDDDVLAPSGATAAGAGDEGDGQTTGST

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
91
Xenopus
83
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H6Z4 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K21 Acetylation
S27 Phosphorylation
S72 Phosphorylation
S74 Phosphorylation
T75 Phosphorylation
S96 Phosphorylation
S100 Phosphorylation
S101 Phosphorylation
S108 Phosphorylation
Y115 Phosphorylation
C116 S-Nitrosylation
T124 Phosphorylation
S125 Phosphorylation
S126 Phosphorylation P51812 (RPS6KA3) , P31749 (AKT1) , Q15418 (RPS6KA1)
T128 Phosphorylation
S133 Phosphorylation
K145 Ubiquitination
K163 Acetylation
S170 Phosphorylation
S186 Phosphorylation
T188 Phosphorylation
S198 Phosphorylation
C203 S-Nitrosylation
S211 Phosphorylation
T214 Phosphorylation
S219 Phosphorylation
S221 Phosphorylation
K233 Ubiquitination
S240 Phosphorylation
S241 Phosphorylation
S244 Phosphorylation
T256 Phosphorylation
S276 Phosphorylation
T292 Phosphorylation
Y298 Phosphorylation
S328 Phosphorylation
S333 Phosphorylation
S353 Phosphorylation
S355 Phosphorylation
S357 Phosphorylation
S358 Phosphorylation
S359 Phosphorylation
T363 Phosphorylation
S372 Phosphorylation
K378 Ubiquitination
K413 Ubiquitination
T457 Phosphorylation
S493 Phosphorylation
Y501 Phosphorylation
S533 Phosphorylation
S539 Phosphorylation

Research Backgrounds

Function:

Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with high levels in testis and heart.

Subunit Structure:

Interacts with CHC1 in a Ran-stimulated manner. Interacts with XPO1. Interacts (via its C-terminal R domain) with SMAD2 (dephosphorylated form via its MH1 and MH2 domains); the interaction results in the nuclear export of SMAD2 and termination of the TGF-beta signaling. Interacts (via its C-terminal R domain) with SMAD3 (dephosphorylated form via its MH1 domain); the interaction results in the nuclear export of SMAD3 and termination of the TGF-beta signaling.

Research Fields

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.