Product: TOB1 Antibody
Catalog: AF4739
Description: Rabbit polyclonal antibody to TOB1
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 38kDa; 38kD(Calculated).
Uniprot: P50616
RRID: AB_2844738

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TOB1 Antibody detects endogenous levels of total TOB1.
RRID:
AB_2844738
Cite Format: Affinity Biosciences Cat# AF4739, RRID:AB_2844738.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

APRO 6; APRO6; MGC104792; MGC34446; PIG 49; PIG49; Proliferation inducing gene 49; Protein Tob1; RP23 244C22.1; TOB 1; Tob 1 protein; TOB; TOB1; Tob1 protein; Transducer of ERBB 2 1; Transducer of erbB 2; Transducer of ERBB2 1; Transducer of erbB2; Trob; TROB 1; TROB1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P50616 TOB1_HUMAN:

Ubiquitous.

Sequence:
MQLEIQVALNFIISYLYNKLPRRRVNIFGEELERLLKKKYEGHWYPEKPYKGSGFRCIHIGEKVDPVIEQASKESGLDIDDVRGNLPQDLSVWIDPFEVSYQIGEKGPVKVLYVDDNNENGCELDKEIKNSFNPEAQVFMPISDPASSVSSSPSPPFGHSAAVSPTFMPRSTQPLTFTTATFAATKFGSTKMKNSGRSNKVARTSPINLGLNVNDLLKQKAISSSMHSLYGLGLGSQQQPQQQQQPAQPPPPPPPPQQQQQQKTSALSPNAKEFIFPNMQGQGSSTNGMFPGDSPLNLSPLQYSNAFDVFAAYGGLNEKSFVDGLNFSLNNMQYSNQQFQPVMAN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P50616 As Substrate

Site PTM Type Enzyme
Y40 Phosphorylation
K73 Ubiquitination
K110 Ubiquitination
K126 Ubiquitination
S152 Phosphorylation P45984 (MAPK9) , P28482 (MAPK1) , P27361 (MAPK3)
S154 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3) , P45984 (MAPK9)
S164 Phosphorylation P45984 (MAPK9) , P28482 (MAPK1) , P27361 (MAPK3)
T204 Phosphorylation
S205 Phosphorylation
S268 Phosphorylation

Research Backgrounds

Function:

Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex. Mediates CPEB3-accelerated mRNA deadenylation by binding to CPEB3 and recruiting CNOT7 which leads to target mRNA deadenylation and decay.

PTMs:

Phosphorylated on Ser and Thr residues.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Only a small fraction localizes to the cytoplasm except in late S-phase where more than half of proteins become cytoplasmic.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with ERBB2. Interacts with CNOT7. Interacts with CPEB3 (via C-terminal RNA-binding region); recruits CNOT7 to CPEB3 to form a ternary complex required for mRNA deadenylation and decay. Interacts with CNOT8. Interacts with CPEB4.

Family&Domains:

Belongs to the BTG family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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