IFT81 Antibody - #DF12032

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Product: IFT81 Antibody
Catalog: DF12032
Description: Rabbit polyclonal antibody to IFT81
Application: WB IHC
Reactivity: Human, Mouse, Rat, Dog
Prediction: Zebrafish, Bovine, Horse, Dog, Chicken, Xenopus
Mol.Wt.: 75-80 kDa; 80kD(Calculated).
Uniprot: Q8WYA0
RRID: AB_2844837

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Dog
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
IFT81 Antibody detects endogenous levels of total IFT81.
RRID:
AB_2844837
Cite Format: Affinity Biosciences Cat# DF12032, RRID:AB_2844837.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Carnitine deficiency associated expressed in ventricle 1 isoform 2; Carnitine deficiency-associated protein expressed in ventricle 1; CDV-1; CDV1; CDV1R; Ift81; IFT81_HUMAN; intraflagellar transport 81 homolog (Chlamydomonas); Intraflagellar transport protein 81 homolog;

Immunogens

Immunogen:
Uniprot:

Q8WYA0(IFT81_HUMAN) >>Visit HPA database.

Gene(ID):
IFT81(28981)
Expression:
Q8WYA0 IFT81_HUMAN:

Highly expressed in testis, moderately in ovary, heart, liver, skeletal muscle, kidney and pancreas, low in prostate, brain, placenta and lung and not detected in spleen, thymus, small intestine and colon. Isoform CDV-1R is abundantly expressed in testis.

Sequence:
MSDQIKFIMDSLNKEPFRKNYNLITFDSLEPMQLLQVLSDVLAEIDPKQLVDIREEMPEQTAKRMLSLLGILKYKPSGNATDMSTFRQGLVIGSKPVIYPVLHWLLQRTNELKKRAYLARFLIKLEVPSEFLQDETVADTNKQYEELMEAFKTLHKEYEQLKISGFSTAEIRKDISAMEEEKDQLIKRVEHLKKRVETAQNHQWMLKIARQLRVEKEREEYLAQQKQEQKNQLFHAVQRLQRVQNQLKSMRQAAADAKPESLMKRLEEEIKFNLYMVTEKFPKELENKKKELHFLQKVVSEPAMGHSDLLELESKINEINTEINQLIEKKMMRNEPIEGKLSLYRQQASIISRKKEAKAEELQEAKEKLASLEREASVKRNQTREFDGTEVLKGDEFKRYVNKLRSKSTVFKKKHQIIAELKAEFGLLQRTEELLKQRHENIQQQLQTMEEKKGISGYSYTQEELERVSALKSEVDEMKGRTLDDMSEMVKKLYSLVSEKKSALASVIKELRQLRQKYQELTQECDEKKSQYDSCAAGLESNRSKLEQEVRRLREECLQEESRYHYTNCMIKNLEVQLRRATDEMKAYISSDQQEKRKAIREQYTKNTAEQENLGKKLREKQKVIRESHGPNMKQAKMWRDLEQLMECKKQCFLKQQSQTSIGQVIQEGGEDRLIL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Pig
0
Sheep
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WYA0 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K14 Ubiquitination
T61 Phosphorylation
K73 Sumoylation
K75 Sumoylation
K75 Ubiquitination
Y144 Phosphorylation
K152 Ubiquitination
K156 Ubiquitination
Y158 Phosphorylation
K182 Ubiquitination
K187 Ubiquitination
K216 Ubiquitination
K226 Ubiquitination
K230 Ubiquitination
K248 Ubiquitination
S249 Phosphorylation
S261 Phosphorylation
K264 Ubiquitination
K290 Acetylation
K297 Ubiquitination
K329 Ubiquitination
R333 Methylation
K340 Ubiquitination
R345 Methylation
S371 Phosphorylation
S377 Phosphorylation
T383 Phosphorylation
K393 Ubiquitination
K398 Ubiquitination
Y400 Phosphorylation
K414 Ubiquitination
K436 Ubiquitination
K453 Ubiquitination
K472 Ubiquitination
K479 Ubiquitination
K492 Ubiquitination
K501 Ubiquitination
K509 Ubiquitination
K529 Ubiquitination
K545 Ubiquitination
K586 Ubiquitination
Y588 Phosphorylation
K596 Acetylation
K596 Ubiquitination
T605 Phosphorylation
K606 Ubiquitination
S628 Phosphorylation
K649 Ubiquitination
S661 Phosphorylation

Research Backgrounds

Function:

Component of the intraflagellar transport (IFT) complex B: together with IFT74, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds tubulin via its CH (calponin-homology)-like region. Required for ciliogenesis. Required for proper regulation of SHH signaling.

Subcellular Location:

Cell projection>Cilium.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis, moderately in ovary, heart, liver, skeletal muscle, kidney and pancreas, low in prostate, brain, placenta and lung and not detected in spleen, thymus, small intestine and colon. Isoform CDV-1R is abundantly expressed in testis.

Subunit Structure:

Component of the IFT complex B, at least composed of IFT20, IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74, IFT80, IFT81, and IFT88. Interacts with IFT74; the interaction is direct: within the IFT complex B, IFT74 and IFT81 mediate the transport of tubulin within the cilium. Interacts with tubulin; the interaction is direct. Interacts with RABL2/RABL2A; binding is equal in the presence of GTP or GDP (By similarity). Interacts with IFT88. Interacts (via the IFT74/IFT81 heterodimer) with RABL2B.

Family&Domains:

The CH (calponin-homology)-like region shows high similarity to a CH (calponin-homology) domain and mediated binding to the globular domain of tubulin.

Belongs to the IFT81 family.

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