NBR1 Antibody - #DF12049

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Product: NBR1 Antibody
Catalog: DF12049
Description: Rabbit polyclonal antibody to NBR1
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 140 kDa; 107kD(Calculated).
Uniprot: Q14596
RRID: AB_2844854

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
NBR1 Antibody detects endogenous levels of total NBR1.
RRID:
AB_2844854
Cite Format: Affinity Biosciences Cat# DF12049, RRID:AB_2844854.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1A1 3B; 1A13B; B box protein; Cell migration-inducing gene 19 protein; KIAA0049; M17S2; Membrane component chromosome 17 surface marker 2; Membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigen CA125); MIG 19; MIG19; Migration inducing protein 19; NBR 1; Nbr1; NBR1, autophagy cargo receptor; NBR1_HUMAN; Neighbor of BRCA1 gene 1; Neighbor of BRCA1 gene 1 protein; Next to BRCA1 gene 1 protein; Ovarian carcinoma antigen CA125; Protein 1A1-3B;

Immunogens

Immunogen:
Uniprot:

Q14596(NBR1_HUMAN) >>Visit HPA database.

Gene(ID):
NBR1(4077)
Sequence:
MEPQVTLNVTFKNEIQSFLVSDPENTTWADIEAMVKVSFDLNTIQIKYLDEENEEVSINSQGEYEEALKMAVKQGNQLQMQVHEGHHVVDEAPPPVVGAKRLAARAGKKPLAHYSSLVRVLGSDMKTPEDPAVQSFPLVPCDTDQPQDKPPDWFTSYLETFREQVVNETVEKLEQKLHEKLVLQNPSLGSCPSEVSMPTSEETLFLPENQFSWHIACNNCQRRIVGVRYQCSLCPSYNICEDCEAGPYGHDTNHVLLKLRRPVVGSSEPFCHSKYSTPRLPAALEQVRLQKQVDKNFLKAEKQRLRAEKKQRKAEVKELKKQLKLHRKIHLWNSIHGLQSPKSPLGRPESLLQSNTLMLPLQPCTSVMPMLSAAFVDENLPDGTHLQPGTKFIKHWRMKNTGNVKWSADTKLKFMWGNLTLASTEKKDVLVPCLKAGHVGVVSVEFIAPALEGTYTSHWRLSHKGQQFGPRVWCSIIVDPFPSEESPDNIEKGMISSSKTDDLTCQQEETFLLAKEERQLGEVTEQTEGTAACIPQKAKNVASERELYIPSVDLLTAQDLLSFELLDINIVQELERVPHNTPVDVTPCMSPLPHDSPLIEKPGLGQIEEENEGAGFKALPDSMVSVKRKAENIASVEEAEEDLSGTQFVCETVIRSLTLDAAPDHNPPCRQKSLQMTFALPEGPLGNEKEEIIHIAEEEAVMEEEEDEEDEEEEDELKDEVQSQSSASSEDYIIILPECFDTSRPLGDSMYSSALSQPGLERGAEGKPGVEAGQEPAEAGERLPGGENQPQEHSISDILTTSQTLETVPLIPEVVELPPSLPRSSPCVHHHGSPGVDLPVTIPEVSSVPDQIRGEPRGSSGLVNSRQKSYDHSRHHHGSSIAGGLVKGALSVAASAYKALFAGPPVTAQPIISEDQTAALMAHLFEMGFCDRQLNLRLLKKHNYNILQVVTELLQLNNNDWYSQRY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14596 As Substrate

Site PTM Type Enzyme
S60 Phosphorylation
K100 Ubiquitination
K108 Ubiquitination
K109 Ubiquitination
S115 Phosphorylation
S116 Phosphorylation
K126 Ubiquitination
K149 Sumoylation
K149 Ubiquitination
K172 Ubiquitination
K176 Ubiquitination
K274 Ubiquitination
S276 Phosphorylation
T277 Phosphorylation
K291 Ubiquitination
K295 Ubiquitination
K299 Ubiquitination
K313 Acetylation
K317 Ubiquitination
K320 Ubiquitination
K328 Ubiquitination
S334 Phosphorylation
S340 Phosphorylation
K394 Ubiquitination
K399 Ubiquitination
K405 Ubiquitination
K411 Ubiquitination
K426 Ubiquitination
K427 Ubiquitination
K464 Ubiquitination
S486 Phosphorylation
K492 Ubiquitination
K499 Ubiquitination
K515 Ubiquitination
K537 Ubiquitination
K539 Ubiquitination
T581 Phosphorylation
T586 Phosphorylation
S590 Phosphorylation
S596 Phosphorylation
K601 Ubiquitination
K617 Ubiquitination
S622 Phosphorylation
S625 Phosphorylation
K627 Ubiquitination
K629 Ubiquitination
S656 Phosphorylation
T658 Phosphorylation
K672 Ubiquitination
S673 Phosphorylation
K767 Ubiquitination
S824 Phosphorylation
S825 Phosphorylation
S833 Phosphorylation
K887 Ubiquitination
K898 Ubiquitination

Research Backgrounds

Function:

Acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets.

Subcellular Location:

Cytoplasm. Cytoplasmic vesicle>Autophagosome. Lysosome. Cytoplasm>Myofibril>Sarcomere>M line.
Note: In cardiac muscles localizes to the sarcomeric M line (By similarity). Is targeted to lysosomes for degradation (PubMed:19250911).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homooligomer and heterooligomer. Interacts with TRIM55 (By similarity). Interacts with SQSTM1, titin/TTN, RNF29, USP8, SQSTM1, MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAP, GABARAPL1 and GABARAPL2. Binds to ubiquitin and ubiquitinated proteins.

Family&Domains:

The PB1 domain mediates interaction with SQSTM1.

References

1). Bradykinin postconditioning protects rat hippocampal neurons after restoration of spontaneous circulation following cardiac arrest via activation of the AMPK/mTOR signaling pathway. Neural Regeneration Research (PubMed: 35259843) [IF=6.1]

Application: IHC    Species: Rat    Sample: brain tissue

Figure 4 ｜ Effect of bradykinin postconditioning on the immunopositivity of AMPK/ mTOR signaling pathway, autophagy-related proteins, and brain injury marker in rats with restoration of spontaneous circulation, as detected by immunohistochemistry.

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