CEP164 Antibody - #DF12253

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Product: CEP164 Antibody
Catalog: DF12253
Description: Rabbit polyclonal antibody to CEP164
Application: WB IHC
Reactivity: Human, Rat, Dog
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 164 kDa; 164kD(Calculated).
Uniprot: Q9UPV0
RRID: AB_2845058

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat,Dog
Prediction:
Pig(92%), Bovine(92%), Sheep(92%), Rabbit(100%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
CEP164 Antibody detects endogenous levels of total CEP164.
RRID:
AB_2845058
Cite Format: Affinity Biosciences Cat# DF12253, RRID:AB_2845058.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI450905; BC027092; CE164_HUMAN; Centrosomal protein 164kDa; Centrosomal protein of 164 kDa; Cep164; D030051D21; FLJ54767; KIAA1052; MGC38792; mKIAA1052; NPHP15; OTTMUSP00000042044; RGD1560988; RGD1561243;

Immunogens

Immunogen:
Uniprot:

Q9UPV0(CE164_HUMAN) >>Visit HPA database.

Gene(ID):
CEP164(22897)
Expression:
Q9UPV0 CE164_HUMAN:

Expressed in several cell lines.

Sequence:
MAGRPLRIGDQLVLEEDYDETYIPSEQEILEFAREIGIDPIKEPELMWLAREGIVAPLPGEWKPCQDITGDIYYFNFANGQSMWDHPCDEHYRSLVIQERAKLSTSGAIKKKKKKKEKKDKKDRDPPKSSLALGSSLAPVHVPLGGLAPLRGLVDTPPSALRGSQSVSLGSSVESGRQLGELMLPSQGLKTSAYTKGLLGSIYEDKTALSLLGLGEETNEEDEEESDNQSVHSSSEPLRNLHLDIGALGGDFEYEESLRTSQPEEKKDVSLDSDAAGPPTPCKPSSPGADSSLSSAVGKGRQGSGARPGLPEKEENEKSEPKICRNLVTPKADPTGSEPAKASEKEAPEDTVDAGEEGSRREEAAKEPKKKASALEEGSSDASQELEISEHMKEPQLSDSIASDPKSFHGLDFGFRSRISEHLLDVDVLSPVLGGACRQAQQPLGIEDKDDSQSSQDELQSKQSKGLEERLSPPLPHEERAQSPPRSLATEEEPPQGPEGQPEWKEAEELGEDSAASLSLQLSLQREQAPSPPAACEKGKEQHSQAEELGPGQEEAEDPEEKVAVSPTPPVSPEVRSTEPVAPPEQLSEAALKAMEEAVAQVLEQDQRHLLESKQEKMQQLREKLCQEEEEEILRLHQQKEQSLSSLRERLQKAIEEEEARMREEESQRLSWLRAQVQSSTQADEDQIRAEQEASLQKLREELESQQKAERASLEQKNRQMLEQLKEEIEASEKSEQAALNAAKEKALQQLREQLEGERKEAVATLEKEHSAELERLCSSLEAKHREVVSSLQKKIQEAQQKEEAQLQKCLGQVEHRVHQKSYHVAGYEHELSSLLREKRQEVEGEHERRLDKMKEEHQQVMAKAREQYEAEERKQRAELLGHLTGELERLQRAHERELETVRQEQHKRLEDLRRRHREQERKLQDLELDLETRAKDVKARLALLEVQEETARREKQQLLDVQRQVALKSEEATATHQQLEEAQKEHTHLLQSNQQLREILDELQARKLKLESQVDLLQAQSQQLQKHFSSLEAEAQKKQHLLREVTVEENNASPHFEPDLHIEDLRKSLGTNQTKEVSSSLSQSKEDLYLDSLSSHNVWHLLSAEGVALRSAKEFLVQQTRSMRRRQTALKAAQQHWRHELASAQEVAKDPPGIKALEDMRKNLEKETRHLDEMKSAMRKGHNLLKKKEEKLNQLESSLWEEASDEGTLGGSPTKKAVTFDLSDMDSLSSESSESFSPPHREWWRQQRIDSTPSLTSRKIHGLSHSLRQISSQLSSVLSILDSLNPQSPPPLLASMPAQLPPRDPKSTPTPTYYGSLARFSALSSATPTSTQWAWDSGQGPRLPSSVAQTVDDFLLEKWRKYFPSGIPLLSNSPTPLESRLGYMSASEQLRLLQHSHSQVPEAGSTTFQGIIEANRRWLERVKNDPRLPLFSSTPKPKATLSLLQLGLDEHNRVKVYRF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
92
Bovine
92
Sheep
92
Dog
92
Chicken
82
Horse
55
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UPV0 As Substrate

Site PTM Type Enzyme
S172 Phosphorylation
S186 Phosphorylation
S201 Phosphorylation
Y203 Phosphorylation
S270 Phosphorylation
S273 Phosphorylation
S286 Phosphorylation
S319 Phosphorylation
S359 Phosphorylation
S407 Phosphorylation
S430 Phosphorylation
S454 Phosphorylation
S455 Phosphorylation
S472 Phosphorylation
S566 Phosphorylation
T568 Phosphorylation
S572 Phosphorylation
S577 Phosphorylation
S588 Phosphorylation
K614 Ubiquitination
K640 Ubiquitination
S646 Phosphorylation
S735 Phosphorylation
K956 Ubiquitination
K969 Ubiquitination
K1010 Ubiquitination
S1013 Phosphorylation
S1022 Phosphorylation
K1027 Ubiquitination
K1038 Ubiquitination
S1054 Phosphorylation
K1068 Ubiquitination
K1076 Ubiquitination
S1085 Phosphorylation
K1114 Ubiquitination
K1132 Methylation
K1150 Ubiquitination
K1176 Ubiquitination
K1181 Ubiquitination
S1205 Phosphorylation
K1216 Ubiquitination
K1217 Ubiquitination
T1253 Phosphorylation
K1260 Ubiquitination
S1267 Phosphorylation
S1273 Phosphorylation
S1284 Phosphorylation
K1359 Ubiquitination
K1362 Ubiquitination
S1372 Phosphorylation
Y1384 Phosphorylation
S1386 Phosphorylation
S1388 Phosphorylation
S1433 Phosphorylation
T1435 Phosphorylation
K1437 Ubiquitination
K1439 Ubiquitination
T1441 Phosphorylation
S1443 Phosphorylation

Research Backgrounds

Function:

Plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA, CHEK2 and CHEK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHEK1.

PTMs:

Phosphorylation at Ser-186 is induced upon DNA-damage caused by treatment with IR irradiation, UV irradiation, hydroxyurea or amphidicolin. Also MDC1-mediated chromatin remodeling is critical for DNA damage-induced phosphorylation.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Nucleus.
Note: Localizes specifically to very distally located appendage structures on the mature centriole from which initiate PC formation (PubMed:26337392). Persisted at centrioles throughout mitosis. Expressed in chromatin-enriched nuclear fraction of HeLa cells. In response to DNA damage, it translocates to nuclear foci that contain the DNA damage response proteins KAT5/TIP60 and CHEK1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in several cell lines.

Subunit Structure:

Interacts (via N-terminus) with ATRIP. Interacts with ATM, ATR and MDC1. Interacts with XPA (via N-terminus) upon UV irradiation. Interacts with CEP83, CCDC92, TTBK2, DVL3, NPHP3 and weakly with NPHP4.

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