MEPCE Antibody - #DF12294

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Product: MEPCE Antibody
Catalog: DF12294
Description: Rabbit polyclonal antibody to MEPCE
Application: WB IHC
Reactivity: Human, Mouse, Rat, Zebrafish
Prediction: Pig, Sheep, Rabbit, Dog
Mol.Wt.: 74 kDa; 74kD(Calculated).
Uniprot: Q7L2J0
RRID: AB_2845099

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Zebrafish
Prediction:
Pig(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MEPCE Antibody detects endogenous levels of total MEPCE.
RRID:
AB_2845099
Cite Format: Affinity Biosciences Cat# DF12294, RRID:AB_2845099.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

7SK snRNA methylphosphate capping enzyme; BCDIN3; Bicoid interacting protein 3 homolog; Bin3 bicoid interacting 3; Bin3 bicoid interacting 3 homolog (Drosophila); Bin3 bicoid interacting 3 homolog; Bin3 homolog; FLJ20257; MePCE; Methylphosphate capping enzyme;

Immunogens

Immunogen:
Uniprot:

Q7L2J0(MEPCE_HUMAN) >>Visit HPA database.

Gene(ID):
MEPCE(56257)
Expression:
Q7L2J0 MEPCE_HUMAN:

Expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. Weakly or not expressed in other tissues.

Sequence:
MIEMAAEKEPFLVPAPPPPLKDESGGGGGPTVPPHQEAASGELRGGTERGPGRCAPSAGSPAAAVGRESPGAAATSSSGPQAQQHRGGGPQAQSHGEARLSDPPGRAAPPDVGEERRGGGGTELGPPAPPRPRNGYQPHRPPGGGGGKRRNSCNVGGGGGGFKHPAFKRRRRVNSDCDSVLPSNFLLGGNIFDPLNLNSLLDEEVSRTLNAETPKSSPLPAKGRDPVEILIPKDITDPLSLNTCTDEGHVVLASPLKTGRKRHRHRGQHHQQQQAAGGSESHPVPPTAPLTPLLHGEGASQQPRHRGQNRDAPQPYELNTAINCRDEVVSPLPSALQGPSGSLSAPPAASVISAPPSSSSRHRKRRRTSSKSEAGARGGGQGSKEKGRGSWGGRHHHHHPLPAAGFKKQQRKFQYGNYCKYYGYRNPSCEDGRLRVLKPEWFRGRDVLDLGCNVGHLTLSIACKWGPSRMVGLDIDSRLIHSARQNIRHYLSEELRLPPQTLEGDPGAEGEEGTTTVRKRSCFPASLTASRGPIAAPQVPLDGADTSVFPNNVVFVTGNYVLDRDDLVEAQTPEYDVVLCLSLTKWVHLNWGDEGLKRMFRRIYRHLRPGGILVLEPQPWSSYGKRKTLTETIYKNYYRIQLKPEQFSSYLTSPDVGFSSYELVATPHNTSKGFQRPVYLFHKARSPSH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Rabbit
100
Horse
0
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7L2J0 As Substrate

Site PTM Type Enzyme
M1 Acetylation
C54 S-Nitrosylation
S57 Phosphorylation
S60 Phosphorylation
S69 Phosphorylation
S78 Phosphorylation
R86 Methylation
S94 Phosphorylation
S101 Phosphorylation
R117 Methylation
K148 Acetylation
S152 Phosphorylation
K163 Ubiquitination
S175 Phosphorylation
S179 Phosphorylation
S183 Phosphorylation
T208 Phosphorylation
T213 Phosphorylation
S216 Phosphorylation
S217 Phosphorylation
K222 Ubiquitination
K233 Ubiquitination
S240 Phosphorylation
T243 Phosphorylation
T245 Phosphorylation
S254 Phosphorylation
K257 Ubiquitination
T258 Phosphorylation
R266 Methylation
T287 Phosphorylation
T291 Phosphorylation
S300 Phosphorylation
R304 Methylation
Y316 Phosphorylation
S330 Phosphorylation
S334 Phosphorylation
S344 Phosphorylation
S358 Phosphorylation
S369 Phosphorylation
R377 Methylation
K384 Methylation
S390 Phosphorylation
K412 Ubiquitination
Y418 Phosphorylation
K438 Ubiquitination
K627 Ubiquitination
T628 Phosphorylation
K635 Ubiquitination
Y661 Phosphorylation

Research Backgrounds

Function:

S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it.

Tissue Specificity:

Expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. Weakly or not expressed in other tissues.

Subunit Structure:

Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, MEPCE/BCDIN3, SART3 proteins and 7SK and U6 snRNAs Ref.24). Interacts with METTL16. Interacts with RBM7; upon genotoxic stress this interaction is enhanced, triggering the release of inactive P-TEFb complex from the core, yielding to P-TEFb complex activation.

Family&Domains:

Belongs to the methyltransferase superfamily.

Restrictive clause

 

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