TRBP Antibody - #DF12336
| Product: | TRBP Antibody |
| Catalog: | DF12336 |
| Description: | Rabbit polyclonal antibody to TRBP |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 43-46 kDa; 39kD(Calculated). |
| Uniprot: | Q15633 |
| RRID: | AB_2845141 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12336, RRID:AB_2845141.
Fold/Unfold
LOQS; Prbp; RISC loading complex subunit TARBP2; RISC-loading complex subunit tarbp2; TAR (HIV 1) RNA binding protein 2; TAR (HIV) RNA binding protein 2; TAR (HIV) RNA-binding protein TRBP1; TAR RNA Binding Protein 2; TAR RNA-binding protein 2; tarbp2; TARBP2 RISC loading complex RNA binding subunit; Trans-activation-responsive RNA-binding protein; TRBP; TRBP1; TRBP2; TRBP2_HUMAN;
Immunogens
- Q15633 TRBP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSEEEQGSGTTTGCGLPSIEQMLAANPGKTPISLLQEYGTRIGKTPVYDLLKAEGQAHQPNFTFRVTVGDTSCTGQGPSKKAAKHKAAEVALKHLKGGSMLEPALEDSSSFSPLDSSLPEDIPVFTAAAAATPVPSVVLTRSPPMELQPPVSPQQSECNPVGALQELVVQKGWRLPEYTVTQESGPAHRKEFTMTCRVERFIEIGSGTSKKLAKRNAAAKMLLRVHTVPLDARDGNEVEPDDDHFSIGVGSRLDGLRNRGPGCTWDSLRNSVGEKILSLRSCSLGSLGALGPACCRVLSELSEEQAFHVSYLDIEELSLSGLCQCLVELSTQPATVCHGSATTREAARGEAARRALQYLKIMAGSK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15633 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T30 | Phosphorylation | Uniprot | |
| K52 | Sumoylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| S156 | Phosphorylation | Uniprot | |
| Y178 | Phosphorylation | Uniprot | |
| T179 | Phosphorylation | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| S286 | Phosphorylation | Uniprot | |
| K360 | Ubiquitination | Uniprot |
Research Backgrounds
Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1.
(Microbial infection) Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR. Mediates recruitment of FTSJ3 methyltransferase to HIV-1 RNA, leading to 2'-O-methylation of the viral genome, allowing HIV-1 to escape the innate immune system.
Cytoplasm. Cytoplasm>Perinuclear region. Nucleus.
Self-associates. Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with EIF2AK2/PKR and inhibits its protein kinase activity. Interacts with DHX9 and PRKRA. Interacts with DICER1, AGO2, MOV10, EIF6 and RPL7A (60S ribosome subunit); they form a large RNA-induced silencing complex (RISC).
(Microbial infection) Interacts with FTSJ3; forms a complex with FTSJ3 and HIV-1 TAR RNA.
Belongs to the TARBP2 family.
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