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$280 100ul
$350 200ul

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  • Product Name
    CALCOCO2 Antibody
  • Catalog No.
    DF12360
  • RRID
    AB_2845165
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC
  • Reactivity
    Human, Mouse, Rat
  • UniProt
  • Mol.Wt
    52kD;
    52kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

Antigen nuclear dot 52 kDa protein; CACO2_HUMAN; Calcium binding and coiled coil domain 2; Calcium binding and coiled coil domain containing protein 2; Calcium-binding and coiled-coil domain-containing protein 2; CALCOCO 2; CALCOCO2; MGC17318; NDP52; Nuclear domain 10 protein 52; Nuclear domain 10 protein; Nuclear domain 10 protein NDP52; Nuclear domain protein 52; Nuclear dot protein 52; OTTHUMP00000218344; OTTHUMP00000218345; OTTHUMP00000218346; OTTHUMP00000218347; OTTHUMP00000218349; OTTHUMP00000218351;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

CALCOCO2 Antibody detects endogenous levels of total CALCOCO2.

RRID:

AB_2845165
Please cite this product as: Affinity Biosciences Cat# DF12360, RRID:AB_2845165.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human CALCOCO2, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

CALCOCO2

Molecular Weight:

Observed Mol.Wt.: 52kD.
Predicted Mol.Wt.: 52kDa(Calculated)..

Subcellular Location:

Cytoplasm > perinuclear region. Golgi apparatus. Cytoplasm > cytoskeleton. According to PubMed:7540613, localizes to nuclear dots. According to PubMed:9230084 and PubMed:12869526, it is not a nuclear dot-associated protein but localizes predominantly in the cytoplasm with a coarse-grained distribution preferentially close to the nucleus.

Tissue Specificity:

Q13137 CACO2_HUMAN:
Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain.

Sequence:
MEETIKDPPTSAVLLDHCHFSQVIFNSVEKFYIPGGDVTCHYTFTQHFIPRRKDWIGIFRVGWKTTREYYTFMWVTLPIDLNNKSAKQQEVQFKAYYLPKDDEYYQFCYVDEDGVVRGASIPFQFRPENEEDILVVTTQGEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQKKQEELETLQSINKKLELKVKEQKDYWETELLQLKEQNQKMSSENEKMGIRVDQLQAQLSTQEKEMEKLVQGDQDKTEQLEQLKKENDHLFLSLTEQRKDQKKLEQTVEQMKQNETTAMKKQQELMDENFDLSKRLSENEIICNALQRQKERLEGENDLLKRENSRLLSYMGLDFNSLPYQVPTSDEGGARQNPGLAYGNPYSGIQESSSPSPLSIKKCPICKADDICDHTLEQQQMQPLCFNCPICDKIFPATEKQIFEDHVFCHSL

Research Background

Function:

Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens such as Salmonella typhimurium upon entry into the cytosol by targeting LGALS8-associated bacteria for autophagy (PubMed:22246324). Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation (PubMed:23022382, PubMed:25771791). Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C (PubMed:23022382, PubMed:25771791). May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion (PubMed:17635994).

Subcellular Location:

Cytoplasm>Perinuclear region. Cytoplasm>Cytoskeleton. Cytoplasmic vesicle>Autophagosome membrane>Peripheral membrane protein.
Note: According to PubMed:7540613, localizes to nuclear dots. According to PubMed:9230084 and PubMed:12869526, it is not a nuclear dot-associated protein but localizes predominantly in the cytoplasm with a coarse-grained distribution preferentially close to the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain.

Subunit Structure:

Dimer (PubMed:23511477). Part of a complex consisting of CALCOCO2, TAX1BP1 and MYO6 (PubMed:17635994). Interacts with GEMIN4 (PubMed:12869526). Interacts with ATG8 family members MAP1LC3A, MAP1LC3B, GABARAP, GABARAPL1 and GABARAPL2 (PubMed:25771791). Interacts with ATG8 family member MAP1LC3C (PubMed:23022382). Interacts with LGALS8 (PubMed:22246324, PubMed:25771791, PubMed:23511477, PubMed:23386746).

Similarity:

The MYO6-binding domain is required for autophagy-mediated degradation of infecting bacteria such as Salmonella typhimurium, but not for bacteria targeting to autophagosomes.

The CLIR (LC3C-interacting region) motif is required for interaction with MAP1LC3C, but dispensable for CALCOCO2-mediated autophagosome maturation.

The LIR-like motif is required for interaction with MAP1LC3A, MAP1LC3B and GABARAPL2, as well as for CALCOCO2-mediated autophagosome maturation.

The LGALS8-binding domain is essential for the recruitment to cytosol-exposed infecting bacteria.

Belongs to the CALCOCO family.

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Catalog Number :

DF12360-BP
(Blocking peptide available as DF12360-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Rabbit
73%
Horse
73%
Sheep
70%
Pig
64%
Bovine
64%
Chicken
0%
Dog
0%
Xenopus
0%
Zebrafish
0%
High similarity Medium similarity Low similarity No similarity
Q13137 as Substrate
Site PTM Type Enzyme
K87 Ubiquitination
K94 Ubiquitination
Y96 Phosphorylation
K160 Ubiquitination
K168 Ubiquitination
K180 Ubiquitination
K192 Ubiquitination
Y204 Phosphorylation
K213 Ubiquitination
K225 Ubiquitination
S238 Phosphorylation
K242 Ubiquitination
K262 Ubiquitination
S271 Phosphorylation
K281 Ubiquitination
K299 Ubiquitination
K312 Ubiquitination
S315 Phosphorylation
K339 Ubiquitination
S347 Phosphorylation
S355 Phosphorylation
Y358 Phosphorylation
Y376 Phosphorylation
Y380 Phosphorylation
S381 Phosphorylation
S387 Phosphorylation
S388 Phosphorylation
S390 Phosphorylation
K395 Ubiquitination
K396 Ubiquitination
K401 Ubiquitination
S445 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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