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$280 100ul
$350 200ul

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  • Product Name
    NSDHL Antibody
  • Catalog No.
    DF12432
  • RRID
    AB_2845237
  • Source
    Rabbit
  • Application
    WB,IHC
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig(100%), Zebrafish(92%), Bovine(92%), Horse(83%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(92%)
  • UniProt
  • Mol.Wt
    42kD,40kD;
    42kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

decarboxylating; H105E3; H105e3 protein; NAD(P) dependent steroid dehydrogenase like; NSDHL; NSDHL_HUMAN; Protein H105e3; SDR31E1; Short chain dehydrogenase/reductase family 31E member 1; Sterol 4 alpha carboxylate 3 dehydrogenase decarboxylating; Sterol-4-alpha-carboxylate 3-dehydrogenase; XAP104;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig(100%), Zebrafish(92%), Bovine(92%), Horse(83%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(92%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

NSDHL Antibody detects endogenous levels of total NSDHL.

RRID:

AB_2845237
Please cite this product as: Affinity Biosciences Cat# DF12432, RRID:AB_2845237.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human NSDHL, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

NSDHL

Molecular Weight:

Observed Mol.Wt.: 42kD,40kD.
Predicted Mol.Wt.: 42kDa(Calculated)..

Tissue Specificity:

Q15738 NSDHL_HUMAN:
Brain, heart, liver, lung, kidney, skin and placenta.

Sequence:
MEPAVSEPMRDQVARTHLTEDTPKVNADIEKVNQNQAKRCTVIGGSGFLGQHMVEQLLARGYAVNVFDIQQGFDNPQVRFFLGDLCSRQDLYPALKGVNTVFHCASPPPSSNNKELFYRVNYIGTKNVIETCKEAGVQKLILTSSASVIFEGVDIKNGTEDLPYAMKPIDYYTETKILQERAVLGANDPEKNFLTTAIRPHGIFGPRDPQLVPILIEAARNGKMKFVIGNGKNLVDFTFVENVVHGHILAAEQLSRDSTLGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWVAYYLALLLSLLVMVISPVIQLQPTFTPMRVALAGTFHYYSCERAKKAMGYQPLVTMDDAMERTVQSFRHLRRVK

Research Background

Function:

Involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein. Lipid droplet.
Note: Trafficking through the Golgi is necessary for ER membrane localization.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Brain, heart, liver, lung, kidney, skin and placenta.

Similarity:

Belongs to the 3-beta-HSD family.

Research Fields

Research Fields:

· Metabolism > Lipid metabolism > Steroid biosynthesis.
· Metabolism > Global and overview maps > Metabolic pathways.

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Catalog Number :

DF12432-BP
(Blocking peptide available as DF12432-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Dog
100%
Rabbit
100%
Bovine
92%
Sheep
92%
Zebrafish
92%
Chicken
92%
Horse
83%
Xenopus
75%
High similarity Medium similarity Low similarity No similarity
Q15738 as Substrate
Site PTM Type Enzyme
M1 Acetylation
T19 Phosphorylation
T22 Phosphorylation
K24 Ubiquitination
K31 Ubiquitination
S87 Phosphorylation
K96 Ubiquitination
S106 Phosphorylation
K114 Ubiquitination
K126 Ubiquitination
K133 Ubiquitination
S147 Phosphorylation
Y164 Phosphorylation
K167 Ubiquitination
K176 Ubiquitination
K191 Ubiquitination
K232 Ubiquitination
K263 Ubiquitination
T284 Phosphorylation
Y288 Phosphorylation
K345 Ubiquitination
Y349 Phosphorylation
T354 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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