decarboxylating; H105E3; H105e3 protein; NAD(P) dependent steroid dehydrogenase like; NSDHL; NSDHL_HUMAN; Protein H105e3; SDR31E1; Short chain dehydrogenase/reductase family 31E member 1; Sterol 4 alpha carboxylate 3 dehydrogenase decarboxylating; Sterol-4-alpha-carboxylate 3-dehydrogenase; XAP104;
WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Human, Mouse, Rat
Pig(100%), Zebrafish(92%), Bovine(92%), Horse(83%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(92%)
Rabbit
Polyclonal
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
NSDHL Antibody detects endogenous levels of total NSDHL.
AB_2845237
Please cite this product as: Affinity Biosciences Cat# DF12432, RRID:AB_2845237.
Liquid
1mg/ml
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
A synthesized peptide derived from human NSDHL, corresponding to a region within the internal amino acids.
>>Visit The Human Protein Atlas
NSDHL
Observed Mol.Wt.: 42kD,40kD.
Predicted Mol.Wt.: 42kDa(Calculated)..
Q15738 NSDHL_HUMAN:
Brain, heart, liver, lung, kidney, skin and placenta.
MEPAVSEPMRDQVARTHLTEDTPKVNADIEKVNQNQAKRCTVIGGSGFLGQHMVEQLLARGYAVNVFDIQQGFDNPQVRFFLGDLCSRQDLYPALKGVNTVFHCASPPPSSNNKELFYRVNYIGTKNVIETCKEAGVQKLILTSSASVIFEGVDIKNGTEDLPYAMKPIDYYTETKILQERAVLGANDPEKNFLTTAIRPHGIFGPRDPQLVPILIEAARNGKMKFVIGNGKNLVDFTFVENVVHGHILAAEQLSRDSTLGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWVAYYLALLLSLLVMVISPVIQLQPTFTPMRVALAGTFHYYSCERAKKAMGYQPLVTMDDAMERTVQSFRHLRRVK
Involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis.
Endoplasmic reticulum membrane>Single-pass membrane protein. Lipid droplet.
Note: Trafficking through the Golgi is necessary for ER membrane localization.
Brain, heart, liver, lung, kidney, skin and placenta.
Belongs to the 3-beta-HSD family.
· Metabolism > Lipid metabolism > Steroid biosynthesis.
· Metabolism > Global and overview maps > Metabolic pathways.
DF12432-BP
(Blocking peptide available as DF12432-BP)
$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Site | PTM Type | Enzyme | Source |
---|---|---|---|
M1 | Acetylation | Uniprot | |
T19 | Phosphorylation | Uniprot | |
T22 | Phosphorylation | Uniprot | |
K24 | Ubiquitination | Uniprot | |
K31 | Ubiquitination | Uniprot | |
S87 | Phosphorylation | Uniprot | |
K96 | Ubiquitination | Uniprot | |
S106 | Phosphorylation | Uniprot | |
K114 | Ubiquitination | Uniprot | |
K126 | Ubiquitination | Uniprot | |
K133 | Ubiquitination | Uniprot | |
S147 | Phosphorylation | Uniprot | |
Y164 | Phosphorylation | Uniprot | |
K167 | Ubiquitination | Uniprot | |
K176 | Ubiquitination | Uniprot | |
K191 | Ubiquitination | Uniprot | |
K232 | Ubiquitination | Uniprot | |
K263 | Ubiquitination | Uniprot | |
T284 | Phosphorylation | Uniprot | |
Y288 | Phosphorylation | Uniprot | |
K345 | Ubiquitination | Uniprot | |
Y349 | Phosphorylation | Uniprot | |
T354 | Phosphorylation | Uniprot |