Putative Ras related protein Rab 12; RAB12; RAB12, member RAS oncogene family; RAB12_HUMAN; Ras-related protein Rab-12;
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
RAB12 Antibody detects endogenous levels of total RAB12.
Please cite this product as: Affinity Biosciences Cat# DF12459, RRID:AB_2845264.
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
A synthesized peptide derived from human RAB12, corresponding to a region within C-terminal amino acids.
Observed Mol.Wt.: 28 kD.
Predicted Mol.Wt.: 27kDa(Calculated)..
Golgi apparatus membrane.
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy (By similarity).
Phosphorylation of Ser-106 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.
Recycling endosome membrane>Lipid-anchor>Cytoplasmic side. Lysosome membrane>Lipid-anchor>Cytoplasmic side. Golgi apparatus membrane. Cytoplasmic vesicle>Autophagosome.
Interacts with RABIF. Interacts with OPTN (By similarity). Interacts with LRRK2; interaction facilitates phosphorylation of Ser-106. Interacts with GDI1, GDI2, CHM and CHML; these interactions are disrupted by phosphorylation on Ser-106. Interacts with RILPL1 and RILPL2; these interactions are dependent on phosphorylation of Ser-106.
Belongs to the small GTPase superfamily. Rab family.
(Blocking peptide available as DF12459-BP)
Tips: For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
This product is for research use only. Not for use in diagnostic or therapeutic procedures.