Phospho-Synapsin I (Ser605) Antibody - #AF2416

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Product: Phospho-Synapsin I (Ser605) Antibody
Catalog: AF2416
Description: Rabbit polyclonal antibody to Phospho-Synapsin I (Ser605)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 75-90kDa; 74kD(Calculated).
Uniprot: P17600
RRID: AB_2845430

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(91%)
Clonality:
Polyclonal
Specificity:
Phospho-Synapsin I (Ser605) Antibody detects endogenous levels of Synapsin I only when phosphorylated at Ser605.
RRID:
AB_2845430
Cite Format: Affinity Biosciences Cat# AF2416, RRID:AB_2845430.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Brain protein 4.1; SYN 1; SYN 1a; SYN 1b; SYN I; SYN1; SYN1_HUMAN; SYN1a; SYN1b; Synapsin 1; Synapsin I; Synapsin-1; Synapsin1; SynapsinI; SYNI;

Immunogens

Immunogen:
Uniprot:

P17600(SYN1_HUMAN) >>Visit HPA database.

Gene(ID):
SYN1(6853)
Sequence:
MNYLRRRLSDSNFMANLPNGYMTDLQRPQPPPPPPGAHSPGATPGPGTATAERSSGVAPAASPAAPSPGSSGGGGFFSSLSNAVKQTTAAAAATFSEQVGGGSGGAGRGGAASRVLLVIDEPHTDWAKYFKGKKIHGEIDIKVEQAEFSDLNLVAHANGGFSVDMEVLRNGVKVVRSLKPDFVLIRQHAFSMARNGDYRSLVIGLQYAGIPSVNSLHSVYNFCDKPWVFAQMVRLHKKLGTEEFPLIDQTFYPNHKEMLSSTTYPVVVKMGHAHSGMGKVKVDNQHDFQDIASVVALTKTYATAEPFIDAKYDVRVQKIGQNYKAYMRTSVSGNWKTNTGSAMLEQIAMSDRYKLWVDTCSEIFGGLDICAVEALHGKDGRDHIIEVVGSSMPLIGDHQDEDKQLIVELVVNKMAQALPRQRQRDASPGRGSHGQTPSPGALPLGRQTSQQPAGPPAQQRPPPQGGPPQPGPGPQRQGPPLQQRPPPQGQQHLSGLGPPAGSPLPQRLPSPTSAPQQPASQAAPPTQGQGRQSRPVAGGPGAPPAARPPASPSPQRQAGPPQATRQTSVSGPAPPKASGAPPGGQQRQGPPQKPPGPAGPTRQASQAGPVPRTGPPTTQQPRPSGPGPAGRPKPQLAQKPSQDVPPPATAAAGGPPHPQLNKSQSLTNAFNLPEPAPPRPSLSQDEVKAETIRSLRKSFASLFSD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Rabbit
91
Zebrafish
67
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17600 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation Q14012 (CAMK1) , Q13131 (PRKAA1)
S11 Phosphorylation
S39 Phosphorylation
S62 Phosphorylation P27361 (MAPK3)
S67 Phosphorylation P27361 (MAPK3)
S293 Phosphorylation
Y301 Phosphorylation
S332 Phosphorylation
S427 Phosphorylation
S432 Phosphorylation
T436 Phosphorylation
S438 Phosphorylation Q8TAS1 (UHMK1)
T448 Phosphorylation
S449 Phosphorylation
S551 Phosphorylation P27361 (MAPK3) , Q00535 (CDK5)
S553 Phosphorylation Q00535 (CDK5)
T567 Phosphorylation
S568 Phosphorylation
S605 Phosphorylation
T613 Phosphorylation
S698 Phosphorylation

Research Backgrounds

Function:

Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.

PTMs:

Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal.

Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles.

Subcellular Location:

Cell junction>Synapse. Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1. Isoform Ib interacts with PRNP (By similarity).

Family&Domains:

The A region binds phospholipids with a preference for negatively charged species.

Belongs to the synapsin family.

References

1). CHIP Decline Is Associated With Isoflurane-Induced Neurodegeneration in Aged Mice. Frontiers in Neuroscience (PubMed: 35368262) [IF=4.3]

Application: WB    Species: Mice    Sample: cortex brain tissue

FIGURE 5 Ubiquitin and synaptic protein expression changed in isoflurane-exposed aged mice. (A) Ubiquitin expression was decreased in the cortex brain tissue in the ISO-15 group, while there was no significant difference in synaptic protein SNAP25 expression in the cortex (n = 6, *p < 0.05). (B) In the brain tissue of cortex, there was no significant difference in the expression level of synapsin I and the phosphorylation level of synapsin I S9 and S605 between CON group, ISO-12.5 group, and ISO-15 group. In the brain tissue of the hippocampus, the expression level of synapsin I and the phosphorylation level of synapsin I S9 and S605 were decreased in both the ISO-12.5 group and ISO-15 group, compared with the CON group, and the expression level of CHIP had a dramatic decline in the ISO-15 group (n = 6, *p < 0.05).

Application: WB    Species: mouse    Sample: N2a cells

FIGURE 6 | Decreased CHIP expression altered synaptic protein expression and phosphorylation in N2a cells. (B) Statistical bar chart to show that CHIP, synapsin I, SNAP25, and PSD95 expression were present (n = 6, **p < 0.01, ***p < 0.001).

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