TRA2B Antibody - #DF12519

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Product: TRA2B Antibody
Catalog: DF12519
Description: Rabbit polyclonal antibody to TRA2B
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 34 kDa; 34kD(Calculated).
Uniprot: P62995
RRID: AB_2845481

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TRA2B Antibody detects endogenous levels of total TRA2B.
RRID:
AB_2845481
Cite Format: Affinity Biosciences Cat# DF12519, RRID:AB_2845481.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

arginine/serine-rich 10; Arginine/serine-rich splicing factor 10; hTRA2-beta; SFRS10; Splicing factor; Splicing factor arginine/serine rich 10; SRFS10; TRA-2 beta; TRA2-beta; Tra2b; TRA2B_HUMAN; TRAN2B; Transformer 2 beta homolog; Transformer-2 protein homolog B; Transformer-2 protein homolog beta; Transformer-2-beta;

Immunogens

Immunogen:
Uniprot:

P62995(TRA2B_HUMAN) >>Visit HPA database.

Gene(ID):
TRA2B(6434)
Expression:
P62995 TRA2B_HUMAN:

Highest expression in heart, skeletal muscle and pancreas. Less abundant in kidney, placenta and brain. Lowest expression in kidney and liver.

Sequence:
MSDSGEQNYGERESRSASRSGSAHGSGKSARHTPARSRSKEDSRRSRSKSRSRSESRSRSRRSSRRHYTRSRSRSRSHRRSRSRSYSRDYRRRHSHSHSPMSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGRPTYGSSRRRDYYDRGYDRGYDDRDYYSRSYRGGGGGGGGWRAAQDRDQIYRRRSPSPYYSRGGYRSRSRSRSYSPRRY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62995 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S4 Phosphorylation
S14 Phosphorylation
S16 Phosphorylation
S18 Phosphorylation
S26 Phosphorylation
S29 Phosphorylation
T33 Phosphorylation
S37 Phosphorylation
S39 Phosphorylation
T69 Phosphorylation
S71 Phosphorylation
S81 Phosphorylation
S83 Phosphorylation
S85 Phosphorylation
Y86 Phosphorylation
S87 Phosphorylation
S95 Phosphorylation
S97 Phosphorylation
S99 Phosphorylation
S102 Phosphorylation
T103 Phosphorylation
K140 Ubiquitination
S155 Phosphorylation
K173 Ubiquitination
K176 Ubiquitination
R187 Methylation
K197 Ubiquitination
T201 Phosphorylation
T203 Phosphorylation
Y207 Phosphorylation
R210 Methylation
T212 Phosphorylation
Y213 Phosphorylation
S215 Phosphorylation
S216 Phosphorylation
Y221 Phosphorylation
Y222 Phosphorylation
R224 Methylation
Y226 Phosphorylation
R228 Methylation
Y230 Phosphorylation
R233 Methylation
Y235 Phosphorylation
Y236 Phosphorylation
S237 Phosphorylation
R238 Methylation
Y240 Phosphorylation
R241 Methylation
R251 Methylation
R256 Methylation
Y260 Phosphorylation
S264 Phosphorylation
S266 Phosphorylation
Y268 Phosphorylation
Y269 Phosphorylation
S270 Phosphorylation
R271 Methylation
Y274 Phosphorylation
S276 Phosphorylation
S278 Phosphorylation
S280 Phosphorylation
S282 Phosphorylation
Y283 Phosphorylation
S284 Phosphorylation
Y288 Phosphorylation

Research Backgrounds

Function:

Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA.

PTMs:

Phosphorylated in the RS domains.

Dimethylation at Arg-241 is probably asymmetric.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest expression in heart, skeletal muscle and pancreas. Less abundant in kidney, placenta and brain. Lowest expression in kidney and liver.

Subunit Structure:

Interacts with SAFB/SAFB1 (By similarity). Found in a pre-mRNA exonic splicing enhancer (ESE) complex with TRA2B/SFRS10, SNRNP70, SNRPA1 and SRRM1. Binds to A3 enhancer proteins SFRS4, SFRS5, SFRS6 and SFRS9. Interacts with CPSF6, RBMY1A1, RBMX, RNPS1 and phosphorylated SFRS13A.

Family&Domains:

Belongs to the splicing factor SR family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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