NUCL Antibody - #DF12542

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Product: NUCL Antibody
Catalog: DF12542
Description: Rabbit polyclonal antibody to NUCL
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 110 kDa; 77kD(Calculated).
Uniprot: P19338
RRID: AB_2845504

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Sheep(100%), Rabbit(83%), Dog(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
NUCL Antibody detects endogenous levels of total NUCL.
RRID:
AB_2845504
Cite Format: Affinity Biosciences Cat# DF12542, RRID:AB_2845504.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C23; FLJ45706; MS1116; NCL; Nucl; NUCL_HUMAN; Nucleolin; Protein C23;

Immunogens

Immunogen:
Uniprot:

P19338(NUCL_HUMAN) >>Visit HPA database.

Gene(ID):
NCL(4691)
Sequence:
MVKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQKKGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAKAVTTPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDEDEDEDEIEPAAMKAAAAAPASEDEDDEDDEDDEDDDDDEEDDSEEEAMETTPAKGKKAAKVVPVKAKNVAEDEDEEEDDEDEDDDDDEDDEDDDDEDDEEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEGTEPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLPYKVTQDELKEVFEDAAEIRLVSKDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGEKGQNQDYRGGKNSTWSGESKTLVLSNLSYSATEETLQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Xenopus
88
Rabbit
83
Chicken
75
Horse
0
Bovine
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P19338 As Substrate

Site PTM Type Enzyme
K3 Methylation
K9 Acetylation
K15 Acetylation
K16 Acetylation
S28 Phosphorylation
S34 Phosphorylation
S41 Phosphorylation
S42 Phosphorylation
S60 Phosphorylation
K62 Acetylation
K63 Acetylation
K63 Ubiquitination
S67 Phosphorylation P24941 (CDK2)
T69 Phosphorylation
K70 Acetylation
K70 Ubiquitination
K71 Acetylation
K71 Ubiquitination
T76 Phosphorylation P24941 (CDK2)
K79 Acetylation
K79 Ubiquitination
K80 Acetylation
T84 Phosphorylation
K87 Acetylation
K88 Acetylation
T92 Phosphorylation
K95 Acetylation
T97 Phosphorylation
T99 Phosphorylation
K102 Acetylation
K102 Ubiquitination
T105 Phosphorylation
T106 Phosphorylation
K109 Acetylation
K110 Acetylation
K110 Ubiquitination
T113 Phosphorylation
K116 Acetylation
K116 Methylation
K116 Ubiquitination
T121 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
K124 Acetylation
K124 Ubiquitination
K125 Acetylation
K132 Acetylation
K135 Acetylation
S145 Phosphorylation
S153 Phosphorylation
S184 Phosphorylation
S206 Phosphorylation
T213 Phosphorylation
T214 Phosphorylation
K223 Acetylation
K223 Sumoylation
K223 Ubiquitination
K294 Acetylation
K294 Sumoylation
K297 Acetylation
K297 Sumoylation
T301 Phosphorylation
T304 Phosphorylation
T305 Phosphorylation
K318 Acetylation
K318 Ubiquitination
K324 Sumoylation
K324 Ubiquitination
T325 Phosphorylation
S328 Phosphorylation
K333 Acetylation
K333 Methylation
K333 Ubiquitination
R342 Methylation
K348 Ubiquitination
Y351 Phosphorylation
S356 Phosphorylation
K362 Ubiquitination
T367 Phosphorylation
K370 Acetylation
K370 Ubiquitination
K377 Acetylation
K377 Sumoylation
K377 Ubiquitination
K382 Ubiquitination
K387 Acetylation
K388 Acetylation
K398 Acetylation
K398 Ubiquitination
Y402 Phosphorylation
K403 Acetylation
K403 Sumoylation
K403 Ubiquitination
T405 Phosphorylation
K410 Ubiquitination
R420 Methylation
S423 Phosphorylation
S428 Phosphorylation
K429 Ubiquitination
Y433 Phosphorylation
K437 Acetylation
K444 Acetylation
K444 Ubiquitination
K449 Acetylation
K449 Ubiquitination
S458 Phosphorylation
S460 Phosphorylation
Y462 Phosphorylation
Y463 Phosphorylation
T464 Phosphorylation
K467 Acetylation
K467 Ubiquitination
Y473 Phosphorylation
R474 Methylation
K477 Acetylation
K477 Ubiquitination
T480 Phosphorylation
S482 Phosphorylation
K486 Ubiquitination
T487 Phosphorylation
S494 Phosphorylation
S496 Phosphorylation
T498 Phosphorylation
K508 Ubiquitination
K513 Acetylation
K513 Ubiquitination
K521 Ubiquitination
K523 Ubiquitination
Y525 Phosphorylation
S532 Phosphorylation
K537 Ubiquitination
S542 Phosphorylation
C543 S-Nitrosylation
K545 Acetylation
K545 Ubiquitination
R561 Methylation
S563 Phosphorylation
K572 Acetylation
K572 Ubiquitination
K577 Acetylation
K577 Sumoylation
K577 Ubiquitination
S580 Phosphorylation
T583 Phosphorylation
T587 Phosphorylation
K589 Sumoylation
K589 Ubiquitination
S591 Phosphorylation
S595 Phosphorylation
R597 Methylation
T602 Phosphorylation
R604 Methylation
T606 Phosphorylation
S608 Phosphorylation
S609 Phosphorylation
K610 Acetylation
K610 Ubiquitination
S619 Phosphorylation
K624 Acetylation
K624 Ubiquitination
K639 Ubiquitination
T641 Phosphorylation P06493 (CDK1)
K646 Acetylation
K646 Methylation
K646 Ubiquitination
R656 Methylation
R660 Methylation
R666 Methylation
R670 Methylation
R673 Methylation
R679 Methylation
R681 Methylation
R687 Methylation
R691 Methylation
R694 Methylation
T707 Phosphorylation P06493 (CDK1)

Research Backgrounds

Function:

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

PTMs:

Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).

Symmetrically methylated by PRMT5.

Subcellular Location:

Nucleus>Nucleolus. Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the SWAP complex that consists of NPM1, NCL/nucleolin, PARP1 and SWAP70 (By similarity). Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts with AICDA (By similarity). Interacts with APTX. Interacts with C1QBP. Interacts with ERBB4. Interacts (via C-terminus) with FMR1 isoform 6 (via N-terminus). Interacts with GZF1; this interaction is important for nucleolar localization of GZF1. Interacts with NSUN2. Interacts with NVL. Interacts (via N-terminus domain) with SETX. Interacts (via RRM1 and C-terminal RRM4/Arg/Gly-rich domains) with TERT; the interaction is important for nucleolar localization of TERT. Interacts with WDR46. Interacts with ZFP36. Interacts with LRRC34 (By similarity). Interacts with RRP1B. Interacts with HNRNPU; this interaction occurs during mitosis. Interacts with RIOK1; RIOK1 recruits NCL to PRMT5 for symmetrically methylation. Interacts with ZBTB7B (By similarity). Interacts with MDK; this interaction promotes NCL clustering and lateral movements of this complex into lipid rafts leading to MDK internalization. Interacts with HDGF (isoform 1).

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

References

1). A novel lncRNA SNHG29 regulates EP300- related histone acetylation modification and inhibits FLT3-ITD AML development. Leukemia (PubMed: 37157016) [IF=11.4]
2). Dimethyl itaconate inhibits LPS‑induced microglia inflammation and inflammasome‑mediated pyroptosis via inducing autophagy and regulating the Nrf‑2/HO‑1 signaling pathway. Molecular Medicine Reports (PubMed: 34296312) [IF=3.4]

Application: WB    Species: Mice    Sample: microglial cells

Figure 5. DI regulates microglia inflammatory profile via inhibiting NF-κB phosphorylation and nuclear translocation. Levels of cytokines, including (A) iNOS, (B) TNF-α, (C) IL-6, (D) IFN-γ, (E) IL-18 and (F) IL-10, were determined by ELISA. (G) Nuclear translocation of p-NF-κB was determined by immunofluorescence staining. Scale bar, 100 µm. (H and I) p-NF-κB expression in the nucleus were determined by western blotting. (J and K) Ratio of whole cell p-NF-κB/total NF-κB expression was determined by western blotting. Data are presented as the mean ± standard error of the mean (n=3). **P<0.01, ***P<0.001 vs. sham group; #P<0.05, ##P<0.01, ###P<0.001 vs. LPS+ATP group. DI, dimethyl itaconate; iNOS, inducible nitric oxide synthase; LPS, lipopolysaccharide; p-, phosphorylated.

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