Product: HIBADH Antibody
Catalog: DF12632
Description: Rabbit polyclonal antibody to HIBADH
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 35 kDa; 35kD(Calculated).
Uniprot: P31937
RRID: AB_2845594

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(90%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
HIBADH Antibody detects endogenous levels of total HIBADH.
RRID:
AB_2845594
Cite Format: Affinity Biosciences Cat# DF12632, RRID:AB_2845594.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3 hydroxy 2 methylpropanoate:NAD(+) oxidoreductase; 3 hydroxyisobutyrate dehydrogenase; 3 hydroxyisobutyrate dehydrogenase mitochondrial; 3-hydroxyisobutyrate dehydrogenase; 3HIDH_HUMAN; EC 1.1.1.31; HIBADH; MGC40361; mitochondrial; NS5ATP1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P31937 3HIDH_HUMAN:

Detected in skin fibroblasts.

Sequence:
MAASLRLLGAASGLRYWSRRLRPAAGSFAAVCSRSVASKTPVGFIGLGNMGNPMAKNLMKHGYPLIIYDVFPDACKEFQDAGEQVVSSPADVAEKADRIITMLPTSINAIEAYSGANGILKKVKKGSLLIDSSTIDPAVSKELAKEVEKMGAVFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLGCMGSNVVYCGAVGTGQAAKICNNMLLAISMIGTAEAMNLGIRLGLDPKLLAKILNMSSGRCWSSDTYNPVPGVMDGVPSANNYQGGFGTTLMAKDLGLAQDSATSTKSPILLGSLAHQIYRMMCAKGYSKKDFSSVFQFLREEETF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Horse
90
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31937 As Substrate

Site PTM Type Enzyme
S12 Phosphorylation
S18 Phosphorylation
Y63 Phosphorylation
S88 Phosphorylation
K95 Ubiquitination
K121 Acetylation
S127 Phosphorylation
S132 Phosphorylation
S140 Phosphorylation
S160 Phosphorylation
K238 Acetylation
S292 Phosphorylation
K297 Acetylation
Y310 Phosphorylation
K321 Ubiquitination

Research Backgrounds

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in skin fibroblasts.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the HIBADH-related family. 3-hydroxyisobutyrate dehydrogenase subfamily.

Research Fields

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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