SORBS2 Antibody - #DF12745

(2)  
Product: SORBS2 Antibody
Catalog: DF12745
Description: Rabbit polyclonal antibody to SORBS2
Application: WB
Reactivity: Human, Mouse
Prediction: Rabbit
Mol.Wt.: 70 kDa; 124kD(Calculated).
Uniprot: O94875
RRID: AB_2845706

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Rabbit(90%)
Clonality:
Polyclonal
Specificity:
SORBS2 Antibody detects endogenous levels of total SORBS2.
RRID:
AB_2845706
Cite Format: Affinity Biosciences Cat# DF12745, RRID:AB_2845706.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Arg binding protein 2; Arg-binding protein 2; Arg/Abl interacting protein 2; Arg/Abl interacting protein; Arg/Abl-interacting protein 2; ArgBP2; KIAA0777; PRO0618; sorbin and SH3 domain containing 2; Sorbin and SH3 domain-containing protein 2; Sorbin; Sorbs2; SRBS2_HUMAN;

Immunogens

Immunogen:
Uniprot:

O94875(SRBS2_HUMAN) >>Visit HPA database.

Gene(ID):
SORBS2(8470)
Expression:
O94875 SRBS2_HUMAN:

Abundantly expressed in heart. In cardiac muscle cells, located in the Z-disks of sarcomere. Also found, but to a lower extent, in small and large intestine, pancreas, thymus, colon, spleen, prostate, testis, brain, ovary and epithelial cells. In the pancreas, mainly expressed in acinar cells, duct cells and all cell types in islets (at protein level). Tends to be down-regulated in pancreatic adenocarcinomas ans metastases.

Sequence:
MSYYQRPFSPSAYSLPASLNSSIVMQHGTSLDSTDTYPQHAQSLDGTTSSSIPLYRSSEEEKRVTVIKAPHYPGIGPVDESGIPTAIRTTVDRPKDWYKTMFKQIHMVHKPDDDTDMYNTPYTYNAGLYNPPYSAQSHPAAKTQTYRPLSKSHSDNSPNAFKDASSPVPPPHVPPPVPPLRPRDRSSTEKHDWDPPDRKVDTRKFRSEPRSIFEYEPGKSSILQHERPASLYQSSIDRSLERPMSSASMASDFRKRRKSEPAVGPPRGLGDQSASRTSPGRVDLPGSSTTLTKSFTSSSPSSPSRAKGGDDSKICPSLCSYSGLNGNPSSELDYCSTYRQHLDVPRDSPRAISFKNGWQMARQNAEIWSSTEETVSPKIKSRSCDDLLNDDCDSFPDPKVKSESMGSLLCEEDSKESCPMAWGSPYVPEVRSNGRSRIRHRSARNAPGFLKMYKKMHRINRKDLMNSEVICSVKSRILQYESEQQHKDLLRAWSQCSTEEVPRDMVPTRISEFEKLIQKSKSMPNLGDDMLSPVTLEPPQNGLCPKRRFSIEYLLEEENQSGPPARGRRGCQSNALVPIHIEVTSDEQPRAHVEFSDSDQDGVVSDHSDYIHLEGSSFCSESDFDHFSFTSSESFYGSSHHHHHHHHHHHRHLISSCKGRCPASYTRFTTMLKHERARHENTEEPRRQEMDPGLSKLAFLVSPVPFRRKKNSAPKKQTEKAKCKASVFEALDSALKDICDQIKAEKKRGSLPDNSILHRLISELLPDVPERNSSLRALRRSPLHQPLHPLPPDGAIHCPPYQNDCGRMPRSASFQDVDTANSSCHHQDRGGALQDRESPRSYSSTLTDMGRSAPRERRGTPEKEKLPAKAVYDFKAQTSKELSFKKGDTVYILRKIDQNWYEGEHHGRVGIFPISYVEKLTPPEKAQPARPPPPAQPGEIGEAIAKYNFNADTNVELSLRKGDRVILLKRVDQNWYEGKIPGTNRQGIFPVSYVEVVKKNTKGAEDYPDPPIPHSYSSDRIHSLSSNKPQRPVFTHENIQGGGEPFQALYNYTPRNEDELELRESDVIDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
90
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O94875 As Substrate

Site PTM Type Enzyme
Y72 Phosphorylation
T115 Phosphorylation
Y118 Phosphorylation
T120 Phosphorylation
Y122 Phosphorylation
Y129 Phosphorylation
Y146 Phosphorylation
S150 Phosphorylation
S152 Phosphorylation
S154 Phosphorylation
S157 Phosphorylation
S166 Phosphorylation
S187 Phosphorylation
T188 Phosphorylation
S207 Phosphorylation
S211 Phosphorylation
Y215 Phosphorylation
S230 Phosphorylation
Y232 Phosphorylation
S234 Phosphorylation
S235 Phosphorylation
S239 Phosphorylation
S245 Phosphorylation
S248 Phosphorylation
S251 Phosphorylation
S259 Phosphorylation
S273 Phosphorylation
T277 Phosphorylation
S278 Phosphorylation
S287 Phosphorylation
S288 Phosphorylation
T292 Phosphorylation
S294 Phosphorylation
S297 Phosphorylation
S298 Phosphorylation
S299 Phosphorylation
S301 Phosphorylation
S302 Phosphorylation
S304 Phosphorylation
S522 Phosphorylation
S532 Phosphorylation
T535 Phosphorylation
S550 Phosphorylation
S712 Phosphorylation
S750 Phosphorylation
S755 Phosphorylation
S838 Phosphorylation
S841 Phosphorylation
S843 Phosphorylation
T860 Phosphorylation
K875 Acetylation
T889 Phosphorylation
Y891 Phosphorylation
Y901 Phosphorylation
S915 Phosphorylation
Y916 Phosphorylation
T921 Phosphorylation
Y976 Phosphorylation
Y993 Phosphorylation
T1001 Phosphorylation
Y1007 Phosphorylation
S1015 Phosphorylation
S1017 Phosphorylation
S1018 Phosphorylation
S1023 Phosphorylation
S1025 Phosphorylation
T1053 Phosphorylation
T1091 Phosphorylation
Y1096 Phosphorylation

Research Backgrounds

Function:

Adapter protein that plays a role in the assembling of signaling complexes, being a link between ABL kinases and actin cytoskeleton. Can form complex with ABL1 and CBL, thus promoting ubiquitination and degradation of ABL1. May play a role in the regulation of pancreatic cell adhesion, possibly by acting on WASF1 phosphorylation, enhancing phosphorylation by ABL1, as well as dephosphorylation by PTPN12. Isoform 6 increases water and sodium absorption in the intestine and gall-bladder.

PTMs:

Ubiquitinated by CBL.

Dephosphorylated by PTPN12.

Subcellular Location:

Cytoplasm>Perinuclear region. Apical cell membrane. Cell junction>Focal adhesion. Cell projection>Lamellipodium.
Note: Found at the Z-disk sarcomeres, stress fibers, dense bodies and focal adhesion. In pancreatic acinar cells, localized preferentially to the apical membrane. Colocalized with vinculin and filamentous actin at focal adhesions and lamellipodia of pancreatic cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Abundantly expressed in heart. In cardiac muscle cells, located in the Z-disks of sarcomere. Also found, but to a lower extent, in small and large intestine, pancreas, thymus, colon, spleen, prostate, testis, brain, ovary and epithelial cells. In the pancreas, mainly expressed in acinar cells, duct cells and all cell types in islets (at protein level). Tends to be down-regulated in pancreatic adenocarcinomas ans metastases.

Subunit Structure:

Interacts with ABL, CBL, DNM1, DNM2, FLOT1, AFDN, PTK2B/PYK2, SAPAP, SPTAN1, SYNJ1, SYNJ2, VCL/vinculin and WASF (By similarity). Interacts with ABL1/c-Abl, ABL2/v-Abl/Arg, ACTN, CBL and PALLD. Interacts with PTPN12 and WASF1 via its SH3 domains; this interaction may mediate the partial PTPN12 and WASF1 translocation to focal adhesion sites.

Family&Domains:

The first 2 SH3 domains are required for WASF1-binding. All 3 SH3 domains can bind independently to PTPN12.

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