TRIM27 Antibody - #DF12782
Product: | TRIM27 Antibody |
Catalog: | DF12782 |
Description: | Rabbit polyclonal antibody to TRIM27 |
Application: | WB |
Reactivity: | Human, Mouse, Rat, Monkey |
Prediction: | Pig, Bovine, Horse, Sheep, Dog |
Mol.Wt.: | 58 kDa; 58kD(Calculated). |
Uniprot: | P14373 |
RRID: | AB_2845743 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12782, RRID:AB_2845743.
Fold/Unfold
AW538890; DAAP-182E11.3; MGC189472; OTTHUMP00000029314; Ret finger protein; RFP; RFP transforming protein; Rfp1; RING finger protein 76; RNF76; TRI27_HUMAN; TRIM 27; Trim27; Tripartite motif containing 27; Tripartite motif containing protein 27; Tripartite motif protein TRIM27; Tripartite motif-containing protein 27; Zinc finger protein RFP;
Immunogens
- P14373 TRI27_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASGSVAECLQQETTCPVCLQYFAEPMMLDCGHNICCACLARCWGTAETNVSCPQCRETFPQRHMRPNRHLANVTQLVKQLRTERPSGPGGEMGVCEKHREPLKLYCEEDQMPICVVCDRSREHRGHSVLPLEEAVEGFKEQIQNQLDHLKRVKDLKKRRRAQGEQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISHLSSLIAQLEEKQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLIICPMSGIDGFSGHVGNHGHSMETSP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P14373 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K79 | Ubiquitination | Uniprot | |
K98 | Ubiquitination | Uniprot | |
K140 | Ubiquitination | Uniprot | |
K151 | Ubiquitination | Uniprot | |
K181 | Ubiquitination | Uniprot | |
K194 | Ubiquitination | Uniprot | |
T269 | Phosphorylation | Uniprot | |
K276 | Ubiquitination | Uniprot | |
K283 | Ubiquitination | Uniprot | |
K292 | Ubiquitination | Uniprot | |
K297 | Ubiquitination | Uniprot | |
K304 | Ubiquitination | Uniprot | |
S341 | Phosphorylation | Uniprot | |
K380 | Ubiquitination | Uniprot | |
K382 | Ubiquitination | Uniprot | |
S493 | Phosphorylation | Uniprot | |
S499 | Phosphorylation | Uniprot |
Research Backgrounds
E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1, leading to promote endosomal F-actin assembly. Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.
Nucleus. Cytoplasm. Nucleus>PML body. Early endosome.
Note: Nuclear or cytoplasmic depending on the cell type (By similarity). Colocalized with PML and EIF3S6 in nuclear bodies. Recruited to retromer-containing endosomes via interaction with MAGEL2 (PubMed:23452853).
Expressed in testis namely within the seminiferous tubules.
Homomultimerizes. Interacts with PML, EIF3S6, EPC1, CHD4 and EID1. Interacts with MAGED4, MAGEF1 and MAGEL2. Interacts with herpes simplex virus protein ICP0.
The coiled-coil region mediates interaction with EPC1 and CHD4. The B box and coiled-coil domains mediate interaction with PML. The B box and the distal coiled-coil domains mediate homomultimerisation. The B30.2 domain mediates interaction with EIF3S6.
Belongs to the TRIM/RBCC family.
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