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$280 100ul
$350 200ul

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  • Product Name
    Cathepsin L Antibody
  • Catalog No.
    DF12880
  • RRID
    AB_2845841
  • Source
    Rabbit
  • Application
    WB,IF/ICC
  • Reactivity
    Human, Mouse
  • UniProt
  • Mol.Wt
    30 kD;
    38kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

Cathepsin L; cathepsin L, 1 b; Cathepsin L1; Cathepsin L1 light chain; CathepsinL; CATL; CATL1_HUMAN; cb15; CTSL; CTSL1; ctsl1b; FLJ31037; hgg1; Major excreted protein; MEP; MGC123162; wu:fb30g09;

Applications:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

Cathepsin L Antibody detects endogenous levels of total Cathepsin L.

RRID:

AB_2845841
Please cite this product as: Affinity Biosciences Cat# DF12880, RRID:AB_2845841.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human Cathepsin L, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

CTSL

Molecular Weight:

Observed Mol.Wt.: 30 kD.
Predicted Mol.Wt.: 38kDa(Calculated)..

Sequence:
MNPTLILAAFCLGIASATLTFDHSLEAQWTKWKAMHNRLYGMNEEGWRRAVWEKNMKMIELHNQEYREGKHSFTMAMNAFGDMTSEEFRQVMNGFQNRKPRKGKVFQEPLFYEAPRSVDWREKGYVTPVKNQGQCGSCWAFSATGALEGQMFRKTGRLISLSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYSVANDTGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKEGIYFEPDCSSEDMDHGVLVVGYGFESTESDNNKYWLVKNSWGEEWGMGGYVKMAKDRRNHCGIASAASYPTV

Research Background

Function:

Thiol protease important for the overall degradation of proteins in lysosomes (Probable). Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen (By similarity).

Subcellular Location:

Lysosome. Apical cell membrane>Peripheral membrane protein>Extracellular side.
Note: Localizes to the apical membrane of thyroid epithelial cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Dimer of a heavy and a light chain linked by disulfide bonds.

Similarity:

Belongs to the peptidase C1 family.

Research Fields

Research Fields:

· Cellular Processes > Transport and catabolism > Autophagy - animal.(View pathway)
· Cellular Processes > Transport and catabolism > Lysosome.(View pathway)
· Cellular Processes > Transport and catabolism > Phagosome.(View pathway)
· Cellular Processes > Cell growth and death > Apoptosis.(View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Immune diseases > Rheumatoid arthritis.
· Organismal Systems > Immune system > Antigen processing and presentation.(View pathway)

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Catalog Number :

DF12880-BP
(Blocking peptide available as DF12880-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
57%
Bovine
57%
Sheep
55%
Dog
50%
Horse
0%
Xenopus
0%
Zebrafish
0%
Chicken
0%
Rabbit
0%
High similarity Medium similarity Low similarity No similarity
P07711 as Substrate
Site PTM Type Enzyme
K104 Sumoylation
K104 Ubiquitination
K123 Ubiquitination
K216 Ubiquitination
N221 N-Glycosylation
T223 Phosphorylation
K230 Ubiquitination
Y295 Phosphorylation
Y311 Phosphorylation
C322 S-Nitrosylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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