KLK3 Antibody | Affinity Biosciences

WB
Cites

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Western blot analysis of extracts from Mouse testis, using KLK3 Antibody at 1/1000 dilution.

Product:	KLK3 Antibody
Catalog:	AF0246
Description:	Rabbit polyclonal antibody to KLK3
Application:	WB IHC
Reactivity:	Human, Mouse
Mol.Wt.:	34kDa; 29kD(Calculated).
Uniprot:	P07288
RRID:	AB_2833421

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

KLK3 Antibody detects endogenous levels of total KLK3.

RRID:

AB_2833421
Cite Format: Affinity Biosciences Cat# AF0246, RRID:AB_2833421.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

antigen, prostate-specific; APS; Gamma seminoprotein; Gamma-seminoprotein; hK3; Kallikrein 3; Kallikrein related peptidase 3; Kallikrein-3; KLK 3; KLK2A1; Klk3; KLK3_HUMAN; P-30 antigen; P30 antigen; Prostate-specific antigen; Psa; Semenogelase; Seminin;

Immunogens

Immunogen:

Uniprot:

P07288(KLK3_HUMAN) >>Visit HPA database.

Gene(ID):

KLK3(354)

Description:

KLK3 Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Belongs to the peptidase S1 family. Kallikrein subfamily.

Sequence:

P07288 KLK3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP

PTMs - P07288 As Substrate

P07288

Site	PTM Type	Source
T143	Phosphorylation	Uniprot
T167	Phosphorylation	Uniprot
K251	Ubiquitination	Uniprot
K254	Ubiquitination	Uniprot

Research Backgrounds

P07288_KLK3_HUMAN

Function:

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Subcellular Location:

Secreted.

Subunit Structure:

Forms a heterodimer with SERPINA5.

Family&Domains:

Belongs to the peptidase S1 family. Kallikrein subfamily.

Research Fields

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

References

1). Pyrroloquinoline quinone ameliorates liver injury in mice induced by cyclophosphamide. Environmental Science and Pollution Research (PubMed: 34997497) [IF=5.8]

2). Dihydroartemisinin-ursodeoxycholic acid conjugate is a potential treatment agent for inflammatory bowel disease. International Immunopharmacology (PubMed: 36842236) [IF=5.6]

3). Sodium acetate alleviated high-carbohydrate induced intestinal inflammation by suppressing MAPK and NF-κB signaling pathways in Nile tilapia (Oreochromis niloticus). Fish & Shellfish Immunology (PubMed: 31730927) [IF=4.7]

4). Sodium Danshensu Cream Promotes the Healing of Pressure Ulcers in Mice through the Nrf2/HO-1 and NF-κB Pathways. Pharmaceuticals (PubMed: 36558999) [IF=4.6]

Application: WB Species: Mouse Sample:

Figure 7. Effect of SDSS on the protein levels of the NF-κB pathway (n = 5). (A) Western blot; the protein expression levels of NF-κB p65 (B), NF-κB p50 (C), IKKα (D), IKKβ (E), and IκBα (F) levels. * p < 0.05, ** p < 0.01 when compared to the control group. # p < 0.05, ## p < 0.01 when compared to the positive group.

5). Laminaria japonica fucoidan ameliorates cyclophosphamide‐induced liver and kidney injury possibly by regulating Nrf2/HO‐1 and TLR4/NF‐κB signaling pathways. Journal of the Science of Food and Agriculture (PubMed: 34689333) [IF=4.1]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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KLK3 Antibody - #AF0246