CNKSR3 Antibody - #DF12901

(3)  
Product: CNKSR3 Antibody
Catalog: DF12901
Description: Rabbit polyclonal antibody to CNKSR3
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 62 kDa; 62kD(Calculated).
Uniprot: Q6P9H4
RRID: AB_2845862

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(83%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
CNKSR3 Antibody detects endogenous levels of total CNKSR3.
RRID:
AB_2845862
Cite Format: Affinity Biosciences Cat# DF12901, RRID:AB_2845862.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CNK homolog protein 3; CNK3; CNKSR family member 3; Connector enhancer of kinase suppressor of ras 3; Connector enhancer of KSR 3; MAGI1; Maguin like protein; Membrane associated guanylate kinase interacting protein like 1; Membrane associated guanylate kinase, WW and PDZ domain containing 1; RP11 486M3 1; RP11 486M3.1;

Immunogens

Immunogen:
Uniprot:

Q6P9H4(CNKR3_HUMAN) >>Visit HPA database.

Gene(ID):
CNKSR3(154043)
Sequence:
MEPVTKWSPKQVVDWTRGLDDCLQQYVHKFEREKINGEQLLQISHQDLEELGVTRIGHQELVLEAVDLLCALNYGLETDNMKNLVLKLRASSHNLQNYISSRRKSPAYDGNTSRKAPNEFLTSVVELIGAAKALLAWLDRAPFTGITDFSVTKNKIIQLCLDLTTTVQKDCFVAEMEDKVLTVVKVLNGICDKTIRSTTDPVMSQCACLEEVHLPNIKPGEGLGMYIKSTYDGLHVITGTTENSPADRSQKIHAGDEVIQVNQQTVVGWQLKNLVKKLRENPTGVVLLLKKRPTGSFNFTPAPLKNLRWKPPLVQTSPPPATTQSPESTMDTSLKKEKSAILDLYIPPPPAVPYSPRDENGSFVYGGSSKCKQPLPGPKGSESPNSFLDQESRRRRFTIADSDQLPGYSVETNILPTKMREKTPSYGKPRPLSMPADGNWMGIVDPFARPRGHGRKGEDALCRYFSNERIPPIIEESSSPPYRFSRPTTERHLVRGADYIRGSRCYINSDLHSSATIPFQEEGTKKKSGSSATKSSSTEPSLLVSWFTRLKLLTH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
83
Rabbit
83
Zebrafish
78
Xenopus
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6P9H4 As Substrate

Site PTM Type Enzyme
K34 Ubiquitination
S105 Phosphorylation
S113 Phosphorylation
T283 Phosphorylation
T300 Phosphorylation
K305 Ubiquitination
S325 Phosphorylation
S339 Phosphorylation
S355 Phosphorylation
Y365 Phosphorylation
S381 Phosphorylation
S383 Phosphorylation
T398 Phosphorylation
Y426 Phosphorylation
S433 Phosphorylation
S466 Phosphorylation
S479 Phosphorylation
R483 Methylation
R495 Methylation
S503 Phosphorylation
S514 Phosphorylation
S545 Phosphorylation

Research Backgrounds

Function:

Involved in transepithelial sodium transport. Regulates aldosterone-induced and epithelial sodium channel (ENaC)-mediated sodium transport through regulation of ENaC cell surface expression. Acts as a scaffold protein coordinating the assembly of an ENaC-regulatory complex (ERC).

Subcellular Location:

Cytoplasm. Apical cell membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with epithelial sodium channel ENaC. Interacts directly with SCNN1A (ENaC subunit alpha) and SCNN1B (ENaC subunit beta) C-terminal tails. Interacts with ENaC regulatory proteins NEDD4L, RAF1 and SGK1.

Family&Domains:

The PDZ domain is required for interaction with ENaC and SGK1, but not for interaction with NEDDL4 and RAF1.

Belongs to the CNKSR family.

Restrictive clause

 

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