Phospho-TRAP220/MED1 (Thr1457) Antibody

IHC
IF/ICC
pElisa

1/4

AF3446 at 1/100 staining human Breast carcinoma tissue sections by IHC-P.

AF3446 staining HeLa cells by ICC/IF.

AF3446 staining HepG2 cells(4h of LPS treatment) by IF/ICC.

peptide-ELISA analysis of AF3446.

Product:	Phospho-TRAP220/MED1 (Thr1457) Antibody
Catalog:	AF3446
Description:	Rabbit polyclonal antibody to Phospho-TRAP220/MED1 (Thr1457)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	168kDa; 168kD(Calculated).
Uniprot:	Q15648
RRID:	AB_2834888

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(91%)

Clonality:

Polyclonal

Specificity:

Phospho-TRAP220/MED1 (Thr1457) Antibody detects endogenous levels of TRAP220/MED1 only when phosphorylated at Threonine 1457.

RRID:

AB_2834888
Cite Format: Affinity Biosciences Cat# AF3446, RRID:AB_2834888.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Activator-recruited cofactor 205 kDa component; ARC205; CRSP1; CRSP200; DRIP205; DRIP230; MED1; MED1_HUMAN; Mediator complex subunit 1; Mediator of RNA polymerase II transcription subunit 1; p53 regulatory protein RB18A; PBP; Peroxisome proliferator-activated receptor-binding protein; PPAR binding protein; PPAR-binding protein; PPARBP; PPARGBP; RB18A; Thyroid hormone receptor-associated protein complex 220 kDa component; Thyroid receptor-interacting protein 2; TR-interacting protein 2; Trap220; TRIP-2; TRIP2; Vitamin D receptor-interacting protein complex component DRIP205;

Immunogens

Immunogen:

Uniprot:

Q15648(MED1_HUMAN) >>Visit HPA database.

Gene(ID):

MED1(5469)

Expression:

Q15648 MED1_HUMAN:

Ubiquitously expressed.

Description:

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1.

Sequence:

Q15648 MED1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYVSPSDLLDDKTASPIILHENNVSRSLGMNASVTIEGTSAVYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYAPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICSGSNKTKKKKSSRLPPEKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFHDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNNQADTVDFSIISVAGKALAPADLMEHHSGSQGPLLTTGDLGKEKTQKRVKEGNGTSNSTLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSAGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSASTSGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPLDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKDKSKVSTSGSSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Pig

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15648 As Substrate

Q15648

Site	PTM Type	Enzyme	Source
K2	Methylation		Uniprot
S10	Phosphorylation		Uniprot
K15	Acetylation		Uniprot
K15	Ubiquitination		Uniprot
S18	Phosphorylation		Uniprot
K26	Ubiquitination		Uniprot
K145	Ubiquitination		Uniprot
S151	Phosphorylation		Uniprot
K152	Methylation		Uniprot
K168	Ubiquitination		Uniprot
S179	Phosphorylation		Uniprot
K202	Ubiquitination		Uniprot
T212	Phosphorylation		Uniprot
Y224	Phosphorylation		Uniprot
Y225	Phosphorylation		Uniprot
K235	Ubiquitination		Uniprot
S238	Phosphorylation		Uniprot
T262	Phosphorylation		Uniprot
K378	Ubiquitination		Uniprot
K426	Ubiquitination		Uniprot
K431	Ubiquitination		Uniprot
K481	Ubiquitination		Uniprot
K496	Ubiquitination		Uniprot
S503	Phosphorylation		Uniprot
T507	Phosphorylation		Uniprot
T522	Phosphorylation		Uniprot
K537	Ubiquitination		Uniprot
S588	Phosphorylation		Uniprot
S596	Phosphorylation		Uniprot
S620	Phosphorylation		Uniprot
T628	Phosphorylation		Uniprot
S634	Phosphorylation		Uniprot
S663	Phosphorylation		Uniprot
S664	Phosphorylation		Uniprot
S671	Phosphorylation		Uniprot
S672	Phosphorylation		Uniprot
S673	Phosphorylation		Uniprot
S675	Phosphorylation		Uniprot
S682	Phosphorylation		Uniprot
S684	Phosphorylation		Uniprot
T687	Phosphorylation		Uniprot
T704	Phosphorylation	O14757 (CHEK1)	Uniprot
S719	Phosphorylation		Uniprot
S770	Phosphorylation		Uniprot
S771	Phosphorylation		Uniprot
S772	Phosphorylation		Uniprot
S774	Phosphorylation		Uniprot
T780	Phosphorylation		Uniprot
S784	Phosphorylation		Uniprot
S795	Phosphorylation		Uniprot
T805	Phosphorylation		Uniprot
S816	Phosphorylation	Q13315 (ATM)	Uniprot
S848	Phosphorylation		Uniprot
S850	Phosphorylation		Uniprot
S851	Phosphorylation		Uniprot
S853	Phosphorylation		Uniprot
S872	Phosphorylation	Q13315 (ATM)	Uniprot
S874	Phosphorylation	Q13315 (ATM)	Uniprot
S887	Phosphorylation	Q13315 (ATM)	Uniprot
S900	Phosphorylation		Uniprot
T928	Phosphorylation		Uniprot
S951	Phosphorylation		Uniprot
S953	Phosphorylation	Q13315 (ATM)	Uniprot
K965	Acetylation		Uniprot
K973	Acetylation		Uniprot
K991	Acetylation		Uniprot
K994	Acetylation		Uniprot
S1000	Phosphorylation		Uniprot
K1004	Acetylation		Uniprot
K1006	Methylation		Uniprot
K1011	Acetylation		Uniprot
S1021	Phosphorylation		Uniprot
S1023	Phosphorylation		Uniprot
T1032	Phosphorylation	P27361 (MAPK3) , P28482 (MAPK1)	Uniprot
S1036	Phosphorylation		Uniprot
S1040	Phosphorylation		Uniprot
S1042	Phosphorylation		Uniprot
S1045	Phosphorylation		Uniprot
S1049	Phosphorylation		Uniprot
T1051	Phosphorylation		Uniprot
T1057	Phosphorylation		Uniprot
K1076	Acetylation		Uniprot
S1081	Phosphorylation		Uniprot
S1088	Phosphorylation		Uniprot
S1092	Phosphorylation		Uniprot
K1126	Acetylation		Uniprot
S1128	Phosphorylation		Uniprot
Y1132	Phosphorylation		Uniprot
S1133	Phosphorylation		Uniprot
S1134	Phosphorylation	Q13315 (ATM)	Uniprot
S1141	Phosphorylation	Q13315 (ATM)	Uniprot
S1147	Phosphorylation	Q13315 (ATM)	Uniprot
S1149	Phosphorylation		Uniprot
S1155	Phosphorylation		Uniprot
S1156	Phosphorylation		Uniprot
T1159	Phosphorylation		Uniprot
K1160	Methylation		Uniprot
K1173	Acetylation		Uniprot
K1177	Acetylation		Uniprot
S1187	Phosphorylation		Uniprot
K1188	Acetylation		Uniprot
S1192	Phosphorylation		Uniprot
S1207	Phosphorylation	P24941 (CDK2)	Uniprot
K1210	Acetylation		Uniprot
T1215	Phosphorylation	P24941 (CDK2)	Uniprot
S1218	Phosphorylation		Uniprot
S1223	Phosphorylation		Uniprot
S1226	Phosphorylation		Uniprot
S1227	Phosphorylation		Uniprot
S1229	Phosphorylation		Uniprot
S1232	Phosphorylation		Uniprot
S1239	Phosphorylation		Uniprot
S1241	Phosphorylation		Uniprot
S1251	Phosphorylation		Uniprot
S1252	Phosphorylation		Uniprot
S1254	Phosphorylation		Uniprot
S1256	Phosphorylation		Uniprot
T1259	Phosphorylation		Uniprot
S1302	Phosphorylation		Uniprot
K1309	Acetylation		Uniprot
S1347	Phosphorylation		Uniprot
K1354	Acetylation		Uniprot
S1368	Phosphorylation		Uniprot
K1376	Acetylation		Uniprot
T1378	Phosphorylation		Uniprot
T1387	Phosphorylation		Uniprot
S1401	Phosphorylation		Uniprot
S1403	Phosphorylation		Uniprot
Y1431	Phosphorylation		Uniprot
S1433	Phosphorylation		Uniprot
S1437	Phosphorylation		Uniprot
S1439	Phosphorylation		Uniprot
T1440	Phosphorylation		Uniprot
S1449	Phosphorylation		Uniprot
S1451	Phosphorylation		Uniprot
S1453	Phosphorylation		Uniprot
Y1456	Phosphorylation		Uniprot
T1457	Phosphorylation	P27361 (MAPK3) , P28482 (MAPK1) , P78527 (PRKDC)	Uniprot
S1463	Phosphorylation		Uniprot
S1465	Phosphorylation		Uniprot
S1467	Phosphorylation		Uniprot
S1475	Phosphorylation		Uniprot
Y1476	Phosphorylation		Uniprot
S1479	Phosphorylation		Uniprot
S1481	Phosphorylation		Uniprot
S1482	Phosphorylation		Uniprot
K1502	Acetylation		Uniprot
K1504	Acetylation		Uniprot
K1529	Acetylation		Uniprot
S1532	Phosphorylation		Uniprot
S1534	Phosphorylation		Uniprot
S1536	Phosphorylation		Uniprot
S1548	Phosphorylation		Uniprot
T1550	Phosphorylation		Uniprot

Research Backgrounds

Q15648_MED1_HUMAN

Function:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells.

PTMs:

Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylation increases its interaction with PSIP1.

Subcellular Location:

Nucleus.
Note: A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.

Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. This subunit specifically interacts with a number of nuclear receptors in a ligand-dependent fashion including AR, ESR1, ESR2, PPARA, PPARG, RORA, RXRA, RXRG, THRA, THRB and VDR. Interacts with CTNNB1, GABPA, GLI3, PPARGC1A and TP53. Interacts with YWHAH. Interacts with CLOCK; this interaction requires the presence of THRAP3 (By similarity). Interacts with GATA1 and CCAR1. Interacts with NR4A3 (By similarity). Interacts (via IBM motif) with PSIP1 (via IBD domain); phosphorylation increases its affinity for PSIP1.

Family&Domains:

Belongs to the Mediator complex subunit 1 family.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

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Phospho-TRAP220/MED1 (Thr1457) Antibody - #AF3446