IWS1 Antibody - #DF13099

(2)  
Product: IWS1 Antibody
Catalog: DF13099
Description: Rabbit polyclonal antibody to IWS1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 120 kDa; 92kD(Calculated).
Uniprot: Q96ST2
RRID: AB_2846059

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(92%), Horse(88%), Sheep(92%), Rabbit(92%), Dog(100%), Chicken(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
IWS1 Antibody detects endogenous levels of total IWS1.
RRID:
AB_2846059
Cite Format: Affinity Biosciences Cat# DF13099, RRID:AB_2846059.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp761G0123; FLJ10006; FLJ14655; FLJ32319; Interacts with Spt6; iws1; IWS1 homolog; IWS1-like protein; IWS1_HUMAN; IWS1L; MGC126375; MGC126376; OTTHUMP00000162262; Protein IWS1 homolog;

Immunogens

Immunogen:
Uniprot:

Q96ST2(IWS1_HUMAN) >>Visit HPA database.

Gene(ID):
IWS1(55677)
Sequence:
MDSEYYSGDQSDDGGATPVQDERDSGSDGEDDVNEQHSGSDTGSVERHSENETSDREDGLPKGHHVTDSENDEPLNLNASDSESEELHRQKDSDSESEERAEPPASDSENEDVNQHGSDSESEETRKLPGSDSENEELLNGHASDSENEDVGKHPASDSEIEELQKSPASDSETEDALKPQISDSESEEPPRHQASDSENEEPPKPRMSDSESEELPKPQVSDSESEEPPRHQASDSENEELPKPRISDSESEDPPRHQASDSENEELPKPRISDSESEDPPRNQASDSENEELPKPRVSDSESEGPQKGPASDSETEDASRHKQKPESDDDSDRENKGEDTEMQNDSFHSDSHMDRKKFHSSDSEEEEHKKQKMDSDEDEKEGEEEKVAKRKAAVLSDSEDEEKASAKKSRVVSDADDSDSDAVSDKSGKREKTIASDSEEEAGKELSDKKNEEKDLFGSDSESGNEEENLIADIFGESGDEEEEEFTGFNQEDLEEEKGETQVKEAEDSDSDDNIKRGKHMDFLSDFEMMLQRKKSMSGKRRRNRDGGTFISDADDVVSAMIVKMNEAAEEDRQLNNQKKPALKKLTLLPAVVMHLKKQDLKETFIDSGVMSAIKEWLSPLPDRSLPALKIREELLKILQELPSVSQETLKHSGIGRAVMYLYKHPKESRSNKDMAGKLINEWSRPIFGLTSNYKGMTREEREQRDLEQMPQRRRMNSTGGQTPRRDLEKVLTGEEKALRPGDPGFCARARVPMPSNKDYVVRPKWNVEMESSRFQATSKKGISRLDKQMRKFTDIRKKSRSAHAVKISIEGNKMPL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Chicken
100
Bovine
92
Sheep
92
Rabbit
92
Horse
88
Xenopus
88
Zebrafish
78
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96ST2 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S7 Phosphorylation
S11 Phosphorylation
T17 Phosphorylation
S25 Phosphorylation
S27 Phosphorylation
S40 Phosphorylation
S44 Phosphorylation
S49 Phosphorylation
S54 Phosphorylation
T67 Phosphorylation
S69 Phosphorylation
S80 Phosphorylation
S82 Phosphorylation
S84 Phosphorylation
S93 Phosphorylation
S95 Phosphorylation
S97 Phosphorylation
S106 Phosphorylation
S108 Phosphorylation
S118 Phosphorylation
S120 Phosphorylation
S122 Phosphorylation
S131 Phosphorylation
S133 Phosphorylation
S144 Phosphorylation
S146 Phosphorylation
S157 Phosphorylation
S159 Phosphorylation
S167 Phosphorylation
S170 Phosphorylation
S172 Phosphorylation
S183 Phosphorylation
S196 Phosphorylation
S198 Phosphorylation
S209 Phosphorylation
S211 Phosphorylation
S213 Phosphorylation
S222 Phosphorylation
S224 Phosphorylation
S235 Phosphorylation
S237 Phosphorylation
S248 Phosphorylation
S250 Phosphorylation
S252 Phosphorylation
S261 Phosphorylation
S263 Phosphorylation
S274 Phosphorylation
S276 Phosphorylation
S278 Phosphorylation
S287 Phosphorylation
S289 Phosphorylation
S300 Phosphorylation
S302 Phosphorylation
S304 Phosphorylation
S313 Phosphorylation
S315 Phosphorylation
T317 Phosphorylation
S321 Phosphorylation
S329 Phosphorylation
S333 Phosphorylation
T342 Phosphorylation
S348 Phosphorylation
S351 Phosphorylation
S362 Phosphorylation
S363 Phosphorylation
S365 Phosphorylation
S377 Phosphorylation
S398 Phosphorylation
S400 Phosphorylation
S407 Phosphorylation
K409 Acetylation
S411 Phosphorylation
S415 Phosphorylation
S420 Phosphorylation
S422 Phosphorylation
S426 Phosphorylation
S429 Phosphorylation
K434 Acetylation
T435 Phosphorylation
S438 Phosphorylation
S440 Phosphorylation
S449 Phosphorylation
S480 Phosphorylation
S511 Phosphorylation
S513 Phosphorylation
K518 Ubiquitination
K521 Ubiquitination
S527 Phosphorylation
S554 Phosphorylation
T589 Phosphorylation
K600 Acetylation
K604 Acetylation
S621 Phosphorylation
R626 Methylation
S720 Phosphorylation
T721 Phosphorylation
T725 Phosphorylation
Y762 Phosphorylation
K767 Ubiquitination
K783 Methylation
K794 Methylation
K816 Ubiquitination

Research Backgrounds

Function:

Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription.

PTMs:

Phosphorylation increases its interaction with PSIP1.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with SUPT6H; binds preferentially to the POLR2A-bound SUPT6H. Interacts with ALYREF/THOC4, SETD2 and PRMT5. Interacts with HDGFRP2. Interacts (via IBM motif) with PSIP1 (via IBD domain); phosphorylation increases its affinity for PSIP1.

Family&Domains:

Belongs to the IWS1 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.