KANK2 Antibody - #DF13102

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Product: KANK2 Antibody
Catalog: DF13102
Description: Rabbit polyclonal antibody to KANK2
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog, Xenopus
Mol.Wt.: 95 kDa; 91kD(Calculated).
Uniprot: Q63ZY3
RRID: AB_2846062

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
KANK2 Antibody detects endogenous levels of total KANK2.
RRID:
AB_2846062
Cite Format: Affinity Biosciences Cat# DF13102, RRID:AB_2846062.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ANKRD25; Ankyrin repeat domain 25; Ankyrin repeat domain containing protein 25; DKFZp434N161; FLJ20004; KANK 2; KANK2; KIAA1518; Kidney ankyrin repeat containing protein 2; KN motif and ankyrin repeat domain containing protein 2; KN motif and ankyrin repeat domains 2; Matrix remodeling associated protein 3; Matrix remodelling associated 3; MGC119707; MXRA3; SIP; SRC 1 interacting protein; SRC interacting protein; SRC1 interacting protein;

Immunogens

Immunogen:
Uniprot:

Q63ZY3(KANK2_HUMAN) >>Visit HPA database.

Gene(ID):
KANK2(25959)
Expression:
Q63ZY3 KANK2_HUMAN:

Strongly expressed in cervix, colon, heart, kidney and lung. Expressed in kidney glomerular podocytes and mesangial cells (at protein level).

Sequence:
MAQVLHVPAPFPGTPGPASPPAFPAKDPDPPYSVETPYGYRLDLDFLKYVDDIEKGHTLRRVAVQRRPRLSSLPRGPGSWWTSTESLCSNASGDSRHSAYSYCGRGFYPQYGALETRGGFNPRVERTLLDARRRLEDQAATPTGLGSLTPSAAGSTASLVGVGLPPPTPRSSGLSTPVPPSAGHLAHVREQMAGALRKLRQLEEQVKLIPVLQVKLSVLQEEKRQLTVQLKSQKFLGHPTAGRGRSELCLDLPDPPEDPVALETRSVGTWVRERDLGMPDGEAALAAKVAVLETQLKKALQELQAAQARQADPQPQAWPPPDSPVRVDTVRVVEGPREVEVVASTAAGAPAQRAQSLEPYGTGLRALAMPGRPESPPVFRSQEVVETMCPVPAAATSNVHMVKKISITERSCDGAAGLPEVPAESSSSPPGSEVASLTQPEKSTGRVPTQEPTHREPTRQAASQESEEAGGTGGPPAGVRSIMKRKEEVADPTAHRRSLQFVGVNGGYESSSEDSSTAENISDNDSTENEAPEPRERVPSVAEAPQLRPAGTAAAKTSRQECQLSRESQHIPTAEGASGSNTEEEIRMELSPDLISACLALEKYLDNPNALTERELKVAYTTVLQEWLRLACRSDAHPELVRRHLVTFRAMSARLLDYVVNIADSNGNTALHYSVSHANFPVVQQLLDSGVCKVDKQNRAGYSPIMLTALATLKTQDDIETVLQLFRLGNINAKASQAGQTALMLAVSHGRVDVVKALLACEADVNVQDDDGSTALMCACEHGHKEIAGLLLAVPSCDISLTDRDGSTALMVALDAGQSEIASMLYSRMNIKCSFAPMSDDESPTSSSAEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
82
Zebrafish
64
Horse
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q63ZY3 As Substrate

Site PTM Type Enzyme
T14 Phosphorylation
S19 Phosphorylation
K48 Ubiquitination
T58 Phosphorylation
S71 Phosphorylation
S72 Phosphorylation
S83 Phosphorylation
T84 Phosphorylation
S92 Phosphorylation
S95 Phosphorylation
S98 Phosphorylation
Y100 Phosphorylation
S101 Phosphorylation
Y102 Phosphorylation
R105 Methylation
Y111 Phosphorylation
R117 Methylation
R123 Methylation
T141 Phosphorylation
S147 Phosphorylation
S158 Phosphorylation
T168 Phosphorylation
S171 Phosphorylation
S172 Phosphorylation
S175 Phosphorylation
T176 Phosphorylation
K223 Ubiquitination
K231 Methylation
K231 Ubiquitination
S232 Phosphorylation
K234 Ubiquitination
T240 Phosphorylation
R243 Methylation
S246 Phosphorylation
K288 Ubiquitination
K297 Ubiquitination
S323 Phosphorylation
T329 Phosphorylation
S356 Phosphorylation
Y360 Phosphorylation
S375 Phosphorylation
S406 Phosphorylation
S425 Phosphorylation
S463 Phosphorylation
T472 Phosphorylation
T493 Phosphorylation
S540 Phosphorylation
T552 Phosphorylation
S568 Phosphorylation
K603 Ubiquitination
Y702 Phosphorylation
K734 Acetylation
T741 Phosphorylation

Research Backgrounds

Function:

Involved in transcription regulation by sequestering in the cytoplasm nuclear receptor coactivators such as NCOA1, NCOA2 and NCOA3. Involved in regulation of caspase-independent apoptosis by sequestering the proapoptotic factor AIFM1 in mitochondria. Pro-apoptotic stimuli can induce its proteasomal degradation allowing the translocation of AIFM1 to the nucleus to induce apoptosis. Involved in the negative control of vitamin D receptor signaling pathway. Involved in actin stress fibers formation through its interaction with ARHGDIA and the regulation of the Rho signaling pathway. May thereby play a role in cell adhesion and migration, regulating for instance podocytes migration during development of the kidney. Through the Rho signaling pathway may also regulate cell proliferation (By similarity).

PTMs:

Phosphorylated by casein kinase II upon estrogen stimulation. Phosphorylation induces the release by KANK2 of NCOA1 and its translocation to the nucleus where NCOA1 can activate gene transcription.

Subcellular Location:

Cytoplasm. Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Strongly expressed in cervix, colon, heart, kidney and lung. Expressed in kidney glomerular podocytes and mesangial cells (at protein level).

Subunit Structure:

Interacts (non-phosphorylated form) with NCOA1; NCOA2 AND NCOA3. Interacts with AIFM1. Interacts with ARHGDIA; the interaction is direct and may regulate the interaction of ARHGDIA with RHOA, RAC1 and CDC42.

Restrictive clause

 

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