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$280 100ul
$350 200ul

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  • Product Name
    MIPEP Antibody
  • Catalog No.
    DF13151
  • Source
    Rabbit
  • Application
    WB
  • Reactivity:
    Human
  • Prediction:
    Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(83%)
  • UniProt
  • Mol.Wt.
    70-80 kDa
  • Concentration
    1mg/ml
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Product Information

Alternative Names:Expand▼

EC 3.4.24.59; HMIP; MIP; Mipep; MIPEP_HUMAN; Mitochondrial intermediate peptidase; Mitochondrial intermedieate peptide; OTTHUMP00000018121; OTTHUMP00000042292;

Applications:

WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human

Predicted Reactivity:

Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(83%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

MIPEP Antibody detects endogenous levels of MIPEP.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human MIPEP, corresponding to a region within C-terminal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene id:

Molecular Weight:

Observed Mol.Wt.: 70-80 kDa.
Predicted Mol.Wt.: 81kDa.

Sequence:
        10         20         30         40         50
MLCVGRLGGL GARAAALPPR RAGRGSLEAG IRARRVSTSW SPVGAAFNVK
60 70 80 90 100
PQGSRLDLFG ERRGLFGVPE LSAPEGFHIA QEKALRKTEL LVDRACSTPP
110 120 130 140 150
GPQTVLIFDE LSDSLCRVAD LADFVKIAHP EPAFREAAEE ACRSIGTMVE
160 170 180 190 200
KLNTNVDLYQ SLQKLLADKK LVDSLDPETR RVAELFMFDF EISGIHLDKE
210 220 230 240 250
KRKRAVDLNV KILDLSSTFL MGTNFPNKIE KHLLPEHIRR NFTSAGDHII
260 270 280 290 300
IDGLHAESPD DLVREAAYKI FLYPNAGQLK CLEELLSSRD LLAKLVGYST
310 320 330 340 350
FSHRALQGTI AKNPETVMQF LEKLSDKLSE RTLKDFEMIR GMKMKLNPQN
360 370 380 390 400
SEVMPWDPPY YSGVIRAERY NIEPSLYCPF FSLGACMEGL NILLNRLLGI
410 420 430 440 450
SLYAEQPAKG EVWSEDVRKL AVVHESEGLL GYIYCDFFQR ADKPHQDCHF
460 470 480 490 500
TIRGGRLKED GDYQLPVVVL MLNLPRSSRS SPTLLTPSMM ENLFHEMGHA
510 520 530 540 550
MHSMLGRTRY QHVTGTRCPT DFAEVPSILM EYFANDYRVV NQFARHYQTG
560 570 580 590 600
QPLPKNMVSR LCESKKVCAA ADMQLQVFYA TLDQIYHGKH PLRNSTTDIL
610 620 630 640 650
KETQEKFYGL PYVPNTAWQL RFSHLVGYGA RYYSYLMSRA VASMVWKECF
660 670 680 690 700
LQDPFNRAAG ERYRREMLAH GGGREPMLMV EGMLQKCPSV DDFVSALVSD
710
LDLDFETFLM DSE

Background

Function:

Cleaves proteins, imported into the mitochondrion, to their mature size.

Subcellular Location:

Mitochondrion;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Monomer.

Similarity:

Belongs to the peptidase M3 family.

Western blot analysis of extracts from HepG2 cells, using MIPEP Antibody. The lane on the left was treated with blocking peptide.
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Catalog Number :

DF13151-BP
(Blocking peptide available as DF13151-BP)

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Rabbit
100%
Pig
100%
Dog
100%
Horse
100%
Bovine
100%
Chicken
83%
Xenopus
83%
Zebrafish
75%
Sheep
0%
High similarity Medium similarity Low similarity No similarity
Q99797 as Substrate
Site PTM Type Enzyme
K126 Acetylation
K126 Ubiquitination
T147 Phosphorylation
K164 Ubiquitination
T223 Phosphorylation
K231 Ubiquitination
S325 Phosphorylation
S329 Phosphorylation
R331 Methylation
K409 Acetylation
Y510 Phosphorylation
Y579 Phosphorylation
Y628 Phosphorylation
Y632 Phosphorylation
Y633 Phosphorylation
Y635 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.