NMRAL1 Antibody | Affinity Biosciences

1/1

Western blot analysis of extracts from HeLa cells, using NMRAL1 Antibody.

Product:	NMRAL1 Antibody
Catalog:	DF13189
Description:	Rabbit polyclonal antibody to NMRAL1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.:	33 kDa; 33kD(Calculated).
Uniprot:	Q9HBL8
RRID:	AB_2846149

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(82%), Horse(100%), Sheep(90%), Dog(82%), Chicken(80%)

Clonality:

Polyclonal

Specificity:

NMRAL1 Antibody detects endogenous levels of total NMRAL1.

RRID:

AB_2846149
Cite Format: Affinity Biosciences Cat# DF13189, RRID:AB_2846149.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ25918; HSCARG; NmrA like family domain containing 1; NmrA like family domain containing protein 1; NmrA-like family domain-containing protein 1; NMRAL1; NMRL1_HUMAN; SDR48A1;

Immunogens

Immunogen:

Uniprot:

Q9HBL8(NMRL1_HUMAN) >>Visit HPA database.

Gene(ID):

NMRAL1(57407)

Sequence:

Q9HBL8 NMRL1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVDKKLVVVFGGTGAQGGSVARTLLEDGTFKVRVVTRNPRKKAAKELRLQGAEVVQGDQDDQVIMELALNGAYATFIVTNYWESCSQEQEVKQGKLLADLARRLGLHYVVYSGLENIKKLTAGRLAAAHFDGKGEVEEYFRDIGVPMTSVRLPCYFENLLSHFLPQKAPDGKSYLLSLPTGDVPMDGMSVSDLGPVVLSLLKMPEKYVGQNIGLSTCRHTAEEYAALLTKHTRKVVHDAKMTPEDYEKLGFPGARDLANMFRFYALRPDRDIELTLRLNPKALTLDQWLEQHKGDFNLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Pig

Sheep

Bovine

Dog

Chicken

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HBL8 As Substrate

Q9HBL8

Site	PTM Type	Source
K5	Sumoylation	Uniprot
K31	Ubiquitination	Uniprot
K95	Ubiquitination	Uniprot
K133	Ubiquitination	Uniprot
K167	Ubiquitination	Uniprot
S173	Phosphorylation	Uniprot
S177	Phosphorylation	Uniprot
T180	Phosphorylation	Uniprot
S191	Phosphorylation	Uniprot
K206	Ubiquitination	Uniprot
Y207	Phosphorylation	Uniprot
K230	Ubiquitination	Uniprot
K234	Ubiquitination	Uniprot
K240	Ubiquitination	Uniprot
K248	Ubiquitination	Uniprot
K281	Ubiquitination	Uniprot
K293	Ubiquitination	Uniprot

Research Backgrounds

Q9HBL8_NMRL1_HUMAN

Function:

Redox sensor protein. Undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP(+) levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. The homodimer binds one molecule of NADPH. Has higher affinity for NADPH than for NADP(+). Binding to NADPH is necessary to form a stable dimer.

Subcellular Location:

Cytoplasm. Cytoplasm>Perinuclear region. Nucleus.
Note: Under normal redox growth conditions localizes in the cytoplasm and perinuclear region. Nuclear localization is promoted by increased intracellular nitric oxide and reduced NADPH/NADP(+) ratios.

Subunit Structure:

Homodimer. Interacts with ASS1. Interaction is enhanced by low NADPH/NADP(+) ratios, which results in inhibition of ASS1 activity.

Family&Domains:

Belongs to the NmrA-type oxidoreductase family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

NMRAL1 Antibody - #DF13189