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Product Info

Source:
Mouse
Application:
WB(1:500-1:2000), IHC(1:200-1:1000), IF(1:50-1:200)
*The optimal dilutions should be determined by the end user.
Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [AFB17776]
Specificity:
Caspase 3 (p17,Cleaved-Asp175) Antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.
RRID:
AB_2846190
Cite Format: Affinity Biosciences Cat# BF0711, RRID:AB_2846190.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A830040C14Rik; Apopain; CASP-3; CASP3; CASP3_HUMAN; Casp3a; Caspase 3; Caspase 3, apoptosis-related cysteine peptidase; Caspase 3, apoptosis-related cysteine protease; Caspase 3, apoptosis-related cysteine protease a; Caspase-3 subunit p12; CC3; CPP-32; CPP32; CPP32B; Cysteine protease CPP32; EC 3.4.22.56; LICE; mldy; OTTHUMP00000165052; OTTHUMP00000165053; OTTHUMP00000165054; PARP cleavage protease; Procaspase3; protein Yama; SCA 1; SCA-1; SREBP cleavage activity 1; Yama;

Immunogens

Immunogen:

Purified recombinant fragment of human Caspase 3 expressed in E. Coli.

Uniprot:
Gene(ID):
Expression:
P42574 CASP3_HUMAN:

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Sequence:
MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETDSGVDDDMACHKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVATEFESFSFDATFHAKKQIPCIVSMLTKELYFYH

PTMs - P42574 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T4 Phosphorylation
S7 Phosphorylation
S10 Phosphorylation
K11 Acetylation
K11 Ubiquitination
S12 Phosphorylation
K14 Ubiquitination
K19 Ubiquitination
S24 Phosphorylation
S26 Phosphorylation
S29 Phosphorylation
Y41 Phosphorylation
K57 Ubiquitination
S65 Phosphorylation
T67 Phosphorylation
K82 Acetylation
K82 Ubiquitination
K88 Ubiquitination
K105 Ubiquitination
K138 Ubiquitination
S150 Phosphorylation Q16539 (MAPK14)
T152 Phosphorylation
C163 S-Nitrosylation
T174 Phosphorylation
S176 Phosphorylation
K210 Ubiquitination
K229 Ubiquitination
S249 Phosphorylation
K260 Ubiquitination
T270 Phosphorylation

Research Backgrounds

Function:

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

PTMs:

Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.

S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Subunit Structure:

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce.

Family&Domains:

Belongs to the peptidase C14A family.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

References

1). Liang C et al. Π electron-stabilized polymeric micelles potentiate docetaxel therapy in advanced-stage gastrointestinal cancer. Biomaterials 2021 Jan;266:120432. (PubMed: 33069116) [IF=15.304]

2). Liu S et al. Smooth muscle-specific HuR knockout induces defective autophagy and atherosclerosis. Cell Death Dis 2021 Apr 9;12(4):385. (PubMed: 33837179) [IF=9.685]

Application: WB    Species: mouse    Sample: SMCs

Fig. 3| Loss of HuR promoted apoptosis in atherosclerosis. A Immunofluorescent staining of aortic roots from CTR and HuRSMKO mice to determine TUNEL-positive VSMCs. Red puncta denotes TUNEL-positive cells. Green region denotes α-SMA. Scale bar = 20 μm. B Immunohistochemical staining of cleaved caspase-3 in aortic roots (n = 6). Scale bar = 50 μm. C Serum lipid profiles (total cholesterol [TC], triglycerides [TG], high-density lipoprotein cholesterol [HDL-C], and low-density lipoprotein cholesterol [LDL-C]) (n = 6). D Western blot analysis of cleaved caspase-3 in control and HuR-deficient SMCs (n = 5).

3). Feng YK et al. Oral P. gingivalis impairs gut permeability and mediates immune responses associated with neurodegeneration in LRRK2 R1441G mice. J Neuroinflammation 2020 Nov 19;17(1):347. (PubMed: 33213462) [IF=8.322]

Application: WB    Species: mice    Sample: R1441G cells and FVBN cells

Fig.1 c Representative images of western blots of cleaved caspase-3 protein levels and quantitative analysis, which were done with the SN obtained from F + Pg and 1441 + Pg mice compared to F + C and 1441 + C mice. n = 4, **P < 0.01. Two-way ANOVA and Tukey’s test were used for analysis.

4). Li S et al. Fibroblast growth factor 2 contributes to the effect of salidroside on dendritic and synaptic plasticity after cerebral ischemia/reperfusion injury. Aging (Albany NY) 2020 Jun 9;12. (PubMed: 32518214) [IF=5.955]

Application: IF/ICC    Species: rat    Sample:

Figure 8.| Sal attenuates neuronal apoptosis after MCAO/R. (A) Immunofluorescence staining of c-caspase 3 in sections from the ischemic penumbra in each group on day 7 post-MCAO/R (the scale bar is 20 μm).

Application: WB    Species: rat    Sample:

Figure 8.| Sal attenuates neuronal apoptosis after MCAO/R. (A) Immunofluorescence staining of c-caspase 3 in sections from the ischemic penumbra in each group on day 7 post-MCAO/R (the scale bar is 20 μm).. (B–E) Protein expression of c-caspase 3, Bcl-2 and Bax from the ischemic penumbra. (F–H) QPCR results of c-caspase 3, Bcl-2 and Bax expression. Values are expressed as the mean ± SD. #p < 0.05, ##p <0.01 vs. sham; *p < 0.05, **p < 0.01 vs. MCAO/R.

5). Li S et al. PLXNA2 knockdown promotes M2 microglia polarization through mTOR/STAT3 signaling to improve functional recovery in rats after cerebral ischemia/reperfusion injury. Exp Neurol 2021 Dec;346:113854. (PubMed: 34474008) [IF=5.620]

6). Yang YP et al. Tannic Acid Alleviates Lipopolysaccharide-Induced H9C2 Cell Apoptosis by Suppressing ROS-Mediated ER Stress. Mol Med Rep 2021 Jul;24(1):535. (PubMed: 34080663) [IF=3.423]

Application: WB    Species: rat    Sample: H9C2 cells

Figure 2.| TA alleviates the LPS‑induced apoptosis of H9C2 cells. (C) Protein expression levels of Bax, Bcl‑2, cleaved caspase‑9, cleaved caspase‑3 and β‑actin in H9C2 cells treated with LPS, TA or TA + LPS were determined by western blot analysis.

Application: WB    Species: Rat    Sample: H9C2 cells

Figure 6. TA attenuates LPS-induced apoptosis via suppressing ROS-mediated endoplasmic reticulum stress in H9C2 cells. (A) Whole cell lysates were prepared following stimulation with LPS, NAC and TA. The protein expression levels of apoptosis-associated proteins (p-JNK, JNK, Bax, cleaved caspase-9 and cleaved caspase-3) and an anti-apoptotic protein (Bcl-2) were detected in H9C2 cells treated with LPS, NAC + LPS, TA + LPS and TA + NAC + LPS by western blot analysis. Semi-quantification of the protein expression levels of (B) p-JNK/JNK and cleaved caspase-3, and (C) Bax/Bcl-2 and cleaved caspase-9 in H9C2 cells. The mRNA expression levels of (D) Bax, (E) caspase-3, (F) caspase-12 and (G) Cyt c in H9C2 cells normalized to Gapdh. Data are presented as the mean ± standard error of the mean (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. control; #P<0.05 and ##P<0.01 vs. LPS group; §P<0.05 and §§P<0.01 vs. TA + LPS. TA, tannic acid; LPS, lipopolysaccharide; NAC, N-acetylcysteine; p, phosphorylated; JNK, c-Jun N-terminal kinase; Cyt c, cytochrome c.

7). Yang YP et al. Tannic Acid Alleviates Lipopolysaccharide-Induced H9C2 Cell Apoptosis by Suppressing ROS-Mediated ER Stress. Mol Med Rep 2021 Jul;24(1):535. (PubMed: 34080663) [IF=3.423]

8). Chronic oral administration of P. gingivalis induces microglial activation and degeneration of dopaminergic neurons possibly through increase in gut permeability and peripheral IL-17A in LRRK2 R1441G mice.

9). Ketogenic Diet Alleviates Hippocampal Neurodegeneration Via ASIC1a and the Mitochondria-Mediated Apoptotic Pathway in a Rat Model of Temporal Lobe Epilepsy.

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