Fast Myosin Skeletal Heavy chain Antibody - #DF13212

(6)  
Product: Fast Myosin Skeletal Heavy chain Antibody
Catalog: DF13212
Description: Rabbit polyclonal antibody to Fast Myosin Skeletal Heavy chain
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Dog
Mol.Wt.: 223kDa; 223kD(Calculated).
Uniprot: P12882
RRID: AB_2846231

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Fast Myosin Skeletal Heavy chain Antibody detects endogenous levels of total Fast Myosin Skeletal Heavy chain.
RRID:
AB_2846231
Cite Format: Affinity Biosciences Cat# DF13212, RRID:AB_2846231.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

adult 1; MGC133384; MYH1; MYH1_HUMAN; MYHa; MyHC 2x; MyHC 2X/D; MyHC IIx/d; MyHC-2x; MyHC-IIx/d; MYHSA1; Myosin 1; Myosin heavy chain 1; Myosin heavy chain 2x; Myosin heavy chain; Myosin heavy chain IIx/d; Myosin heavy chain, skeletal muscle, adult 1; Myosin, heavy chain 1, skeletal muscle, adult; Myosin, heavy polypeptide 1, skeletal muscle, adult; Myosin-1; skeletal muscle;

Immunogens

Immunogen:
Uniprot:

P12882(MYH1_HUMAN) >>Visit HPA database.

Gene(ID):
MYH1(4619)
Sequence:
MSSDSEMAIFGEAAPFLRKSERERIEAQNKPFDAKTSVFVVDPKESFVKATVQSREGGKVTAKTEAGATVTVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEVTSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIEILGFTSDERVSIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQNLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNAVGALAKAVYDKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKPEAHFSLIHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSAMKTLALLFVGATGAEAEAGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQLITRTQAMCRGFLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEADSLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKIRMDLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEMSGLQSKIEDEQALGMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMETVSKAKGNLEKMCRALEDQLSEIKTKEEEQQRLINDLTAQRARLQTESGEYSRQLDEKDTLVSQLSRGKQAFTQQIEELKRQLEEEIKAKSALAHALQSSRHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILAEWKQKCEETHAELEASQKESRSLSTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQEKSELQAALEEAEASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQMKRNHIRIVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQAILKDTQLHLDDALRSQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNVEAVKGLRKHERKVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNVNLSKFRRIQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12882 As Substrate

Site PTM Type Enzyme
T61 Phosphorylation
Y163 Phosphorylation
T168 Phosphorylation
Y195 Phosphorylation
T198 Phosphorylation
S212 Phosphorylation
K249 Ubiquitination
T258 Phosphorylation
S263 Phosphorylation
Y269 Phosphorylation
K273 Ubiquitination
S294 Phosphorylation
S351 Phosphorylation
Y353 Phosphorylation
T356 Phosphorylation
Y362 Phosphorylation
T381 Phosphorylation
Y389 Phosphorylation
Y404 Phosphorylation
Y413 Phosphorylation
T415 Phosphorylation
K416 Acetylation
Y424 Phosphorylation
K505 Acetylation
Y556 Phosphorylation
T607 Phosphorylation
Y612 Phosphorylation
T628 Phosphorylation
K638 Acetylation
T664 Phosphorylation
Y719 Phosphorylation
S732 Phosphorylation
S742 Phosphorylation
K744 Acetylation
S746 Phosphorylation
T790 Phosphorylation
Y820 Phosphorylation
K857 Methylation
K862 Methylation
S901 Phosphorylation
T915 Phosphorylation
K922 Acetylation
S952 Phosphorylation
K955 Acetylation
K956 Acetylation
T964 Phosphorylation
T983 Phosphorylation
T992 Phosphorylation
T997 Phosphorylation
T1023 Phosphorylation
S1041 Phosphorylation
K1046 Acetylation
K1085 Acetylation
K1087 Acetylation
K1097 Acetylation
R1130 Methylation
S1132 Phosphorylation
S1141 Phosphorylation
S1144 Phosphorylation
T1161 Phosphorylation
S1162 Phosphorylation
T1186 Phosphorylation
T1192 Phosphorylation
T1195 Phosphorylation
S1203 Phosphorylation
S1237 Phosphorylation
K1246 Acetylation
S1261 Phosphorylation
T1265 Phosphorylation
T1278 Phosphorylation
Y1291 Phosphorylation
S1292 Phosphorylation
T1300 Phosphorylation
S1303 Phosphorylation
S1306 Phosphorylation
T1313 Phosphorylation
S1331 Phosphorylation
Y1351 Phosphorylation
S1366 Phosphorylation
S1370 Phosphorylation
T1377 Phosphorylation
Y1379 Phosphorylation
T1381 Phosphorylation
T1387 Phosphorylation
K1394 Acetylation
K1395 Acetylation
K1396 Acetylation
T1421 Phosphorylation
K1448 Acetylation
T1467 Phosphorylation
S1480 Phosphorylation
S1482 Phosphorylation
T1483 Phosphorylation
Y1492 Phosphorylation
T1501 Phosphorylation
S1514 Phosphorylation
T1517 Phosphorylation
S1542 Phosphorylation
S1574 Phosphorylation
S1603 Phosphorylation
T1604 Phosphorylation
S1611 Phosphorylation
Y1647 Phosphorylation
S1667 Phosphorylation
S1702 Phosphorylation
S1714 Phosphorylation
T1722 Phosphorylation
T1725 Phosphorylation
S1726 Phosphorylation
T1730 Phosphorylation
K1731 Acetylation
K1758 Acetylation
T1764 Phosphorylation
K1774 Acetylation
S1832 Phosphorylation
K1835 Acetylation
T1855 Phosphorylation
Y1856 Phosphorylation
K1863 Acetylation
S1898 Phosphorylation
S1919 Phosphorylation

Research Backgrounds

Function:

Muscle contraction.

Subcellular Location:

Cytoplasm>Myofibril.
Note: Thick filaments of the myofibrils.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Family&Domains:

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.