Product: Clathrin Antibody
Catalog: DF13215
Description: Rabbit polyclonal antibody to Clathrin
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 191kDa; 192kD(Calculated).
Uniprot: Q00610
RRID: AB_2846234

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Clathrin Antibody detects endogenous levels of total Clathrin.
RRID:
AB_2846234
Cite Format: Affinity Biosciences Cat# DF13215, RRID:AB_2846234.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Adapter related protein complex 1 beta 1 subunit; Adaptor Protein Complex AP1 Beta 1 Subunit; ADTB1; AP 1 complex subunit beta 1; AP105A; AP1B1; BAM22; Beta Adaptin 1; Beta Prime Adaptin; CLAPB2; Clathrin Assembly Protein Complex 1 Beta Large Chain; Clathrin heavy chain 1; CLH 17; CLH17; CLTC; CLTCL2; Golgi Adaptor HA1/AP1 Adaptin Beta Subunit; KIAA0034; Plasma Membrane Adaptor HA2/AP2 Adaptor Beta Subunit;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANTSGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYRAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q00610 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T31 Phosphorylation
S67 Phosphorylation
S70 Phosphorylation
K78 Ubiquitination
K83 Ubiquitination
K86 Ubiquitination
K96 Ubiquitination
T105 Phosphorylation
R144 Methylation
S146 Phosphorylation
C151 S-Nitrosylation
T158 Phosphorylation
K163 Ubiquitination
S171 Phosphorylation
K189 Ubiquitination
S191 Phosphorylation
K227 Acetylation
K227 Ubiquitination
T235 Phosphorylation
T238 Phosphorylation
K245 Acetylation
K245 Ubiquitination
K246 Ubiquitination
K367 Acetylation
K367 Ubiquitination
Y377 Phosphorylation
K389 Ubiquitination
T394 Phosphorylation
T397 Phosphorylation
S403 Phosphorylation
T411 Phosphorylation
S412 Phosphorylation
Y430 Phosphorylation
K450 Acetylation
K450 Ubiquitination
K453 Ubiquitination
K456 Acetylation
K456 Ubiquitination
T473 Phosphorylation
C491 S-Nitrosylation
K500 Ubiquitination
K506 Acetylation
K507 Ubiquitination
Y510 Phosphorylation
Y556 Phosphorylation
K619 Methylation
K619 Ubiquitination
Y634 Phosphorylation
K637 Acetylation
K637 Ubiquitination
S672 Phosphorylation
C736 S-Nitrosylation
K737 Ubiquitination
K742 Acetylation
K742 Ubiquitination
S751 Phosphorylation
K760 Ubiquitination
K764 Acetylation
K764 Ubiquitination
K798 Ubiquitination
Y799 Phosphorylation
Y803 Phosphorylation
K806 Ubiquitination
S825 Phosphorylation
S841 Phosphorylation
T842 Phosphorylation
K851 Ubiquitination
K856 Acetylation
K856 Ubiquitination
K881 Ubiquitination
Y883 Phosphorylation
Y899 Phosphorylation
Y900 Phosphorylation
S902 Phosphorylation
R903 Methylation
K907 Acetylation
K907 Ubiquitination
K911 Ubiquitination
C918 S-Nitrosylation
C926 S-Nitrosylation
C934 S-Nitrosylation
S938 Phosphorylation
K941 Ubiquitination
S1016 Phosphorylation
S1019 Phosphorylation
K1034 Ubiquitination
K1074 Ubiquitination
Y1096 Phosphorylation
C1102 S-Nitrosylation
S1109 Phosphorylation
K1118 Ubiquitination
K1122 Ubiquitination
K1130 Ubiquitination
Y1137 Phosphorylation
K1155 Acetylation
S1167 Phosphorylation
K1179 Ubiquitination
T1180 Phosphorylation
Y1206 Phosphorylation
K1209 Acetylation
K1209 Ubiquitination
Y1211 Phosphorylation
K1215 Ubiquitination
Y1218 Phosphorylation
S1222 Phosphorylation
R1226 Methylation
S1229 Phosphorylation
T1230 Phosphorylation
Y1237 Phosphorylation
K1254 Ubiquitination
K1264 Acetylation
K1264 Ubiquitination
T1318 Phosphorylation
S1341 Phosphorylation
K1347 Ubiquitination
K1387 Ubiquitination
K1392 Ubiquitination
T1396 Phosphorylation
K1397 Ubiquitination
Y1404 Phosphorylation
Y1405 Phosphorylation
Y1438 Phosphorylation
S1440 Phosphorylation
K1441 Acetylation
K1441 Methylation
K1441 Ubiquitination
K1443 Ubiquitination
K1449 Acetylation
K1449 Ubiquitination
K1461 Ubiquitination
S1462 Phosphorylation
S1466 Phosphorylation
Y1477 Phosphorylation P12931 (SRC)
T1482 Phosphorylation
Y1487 Phosphorylation
S1494 Phosphorylation
K1501 Acetylation
K1501 Ubiquitination
Y1513 Phosphorylation
K1516 Acetylation
K1522 Ubiquitination
S1524 Phosphorylation
K1529 Ubiquitination
K1535 Acetylation
K1535 Ubiquitination
S1544 Phosphorylation
T1608 Phosphorylation
K1609 Ubiquitination
K1612 Ubiquitination
S1618 Phosphorylation
K1621 Acetylation
Y1634 Phosphorylation
Y1657 Phosphorylation
Y1659 Phosphorylation
T1660 Phosphorylation
Y1664 Phosphorylation
Y1673 Phosphorylation
S1674 Phosphorylation

Research Backgrounds

Function:

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation.

Subcellular Location:

Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Coated pit>Peripheral membrane protein>Cytoplasmic side. Melanosome. Cytoplasm>Cytoskeleton>Spindle.
Note: Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium. Interacts with HIP1. Interacts with DENND1A, DENND1B and DENND1C (By similarity). May interact with OCRL (By similarity). Interacts with ERBB2. Interacts with FKBP6. Interacts with CKAP5 and TACC3 forming the TACC3/ch-TOG/clathrin complex located at spindle inter-microtubules bridges; the complex implicates clathrin triskelions; TACC3 and CLTC are proposed to form a composite microtubule interaction surface. Interacts with ATG16L1 (via N-terminus). Interacts with RFTN1; the interaction occurs in response to pathogens. Interacts with USP2 isoform 4.

Family&Domains:

The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It constitutes a major protein-protein interaction node.

Belongs to the clathrin heavy chain family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

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