Prolyl Endopeptidase Antibody - #DF13228

(3)  
Product: Prolyl Endopeptidase Antibody
Catalog: DF13228
Description: Rabbit polyclonal antibody to Prolyl Endopeptidase
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 80kDa; 81kD(Calculated).
Uniprot: P48147
RRID: AB_2846247

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Prolyl Endopeptidase Antibody detects endogenous levels of total Prolyl Endopeptidase.
RRID:
AB_2846247
Cite Format: Affinity Biosciences Cat# DF13228, RRID:AB_2846247.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

dJ355L5.1 (prolyl endopeptidase); HGNC:9358; MGC16060; OTTHUMP00000040498; PE; PEP; Post proline cleaving enzyme; Post-proline cleaving enzyme; PPCE_HUMAN; Prep; Prolyl endopeptidase; Prolyl oligopeptidase;

Immunogens

Immunogen:
Uniprot:

P48147(PPCE_HUMAN) >>Visit HPA database.

Gene(ID):
PREP(5550)
Sequence:
MLSLQYPDVYRDETAVQDYHGHKICDPYAWLEDPDSEQTKAFVEAQNKITVPFLEQCPIRGLYKERMTELYDYPKYSCHFKKGKRYFYFYNTGLQNQRVLYVQDSLEGEARVFLDPNILSDDGTVALRGYAFSEDGEYFAYGLSASGSDWVTIKFMKVDGAKELPDVLERVKFSCMAWTHDGKGMFYNSYPQQDGKSDGTETSTNLHQKLYYHVLGTDQSEDILCAEFPDEPKWMGGAELSDDGRYVLLSIREGCDPVNRLWYCDLQQESSGIAGILKWVKLIDNFEGEYDYVTNEGTVFTFKTNRQSPNYRVINIDFRDPEESKWKVLVPEHEKDVLEWIACVRSNFLVLCYLHDVKNILQLHDLTTGALLKTFPLDVGSIVGYSGQKKDTEIFYQFTSFLSPGIIYHCDLTKEELEPRVFREVTVKGIDASDYQTVQIFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFNISITPNYSVSRLIFVRHMGGILAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYLIKEGYTSPKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKYTIGHAWTTDYGCSDSKQHFEWLVKYSPLHNVKLPEADDIQYPSMLLLTADHDDRVVPLHSLKFIATLQYIVGRSRKQSNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNVDWIP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
88
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48147 As Substrate

Site PTM Type Enzyme
M1 Acetylation
Y19 Phosphorylation
K23 Ubiquitination
Y28 Phosphorylation
K40 Ubiquitination
K48 Ubiquitination
Y71 Phosphorylation
Y73 Phosphorylation
Y90 Phosphorylation
S120 Phosphorylation
K157 Acetylation
K162 Ubiquitination
K172 Ubiquitination
K196 Ubiquitination
K209 Ubiquitination
S241 Phosphorylation
Y246 Phosphorylation
S250 Phosphorylation
K327 Ubiquitination
K428 Ubiquitination
Y435 Phosphorylation
Y442 Phosphorylation
Y510 Phosphorylation
K516 Ubiquitination
K523 Ubiquitination
K539 Ubiquitination
K546 Ubiquitination
T596 Phosphorylation
S615 Phosphorylation
K621 Ubiquitination
R643 Methylation
Y658 Phosphorylation
S667 Phosphorylation

Research Backgrounds

Function:

Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.

PTMs:

The N-terminus is blocked.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer.

Family&Domains:

Belongs to the peptidase S9A family.

Research Fields

· Organismal Systems > Endocrine system > Renin-angiotensin system.   (View pathway)

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