macroH2A.1 Antibody - #DF13300

(4)  
Product: macroH2A.1 Antibody
Catalog: DF13300
Description: Rabbit polyclonal antibody to macroH2A.1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 39kDa; 40kD(Calculated).
Uniprot: O75367
RRID: AB_2846319

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
macroH2A.1 Antibody detects endogenous levels of total macroH2A.1.
RRID:
AB_2846319
Cite Format: Affinity Biosciences Cat# DF13300, RRID:AB_2846319.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Core histone macro h2a.1; Core histone macro-H2A.1; H2A histone family member Y; H2A.y; H2A/y; H2AF12M; H2AFJ; H2afy; H2AY_HUMAN; Histone H2A.Y; Histone macroH2A1; Histone macroH2A1.1; Histone macroH2A1.2; Macroh2a1; MACROH2A1.1; MacroH2A1.2; Medulloblastoma antigen MU MB 50.205; Medulloblastoma antigen MU-MB-50.205; mH2a; mH2A1;

Immunogens

Immunogen:
Uniprot:

O75367(H2AY_HUMAN) >>Visit HPA database.

Gene(ID):
H2AFY(9555)
Expression:
O75367 H2AY_HUMAN:

Widely expressed.

Sequence:
MSSRGGKKKSTKTSRSAKAGVIFPVGRMLRYIKKGHPKYRIGVGAPVYMAAVLEYLTAEILELAGNAARDNKKGRVTPRHILLAVANDEELNQLLKGVTIASGGVLPNIHPELLAKKRGSKGKLEAIITPPPAKKAKSPSQKKPVSKKAGGKKGARKSKKKQGEVSKAASADSTTEGTPADGFTVLSTKSLFLGQKLNLIHSEISNLAGFEVEAIINPTNADIDLKDDLGNTLEKKGGKEFVEAVLELRKKNGPLEVAGAAVSAGHGLPAKFVIHCNSPVWGADKCEELLEKTVKNCLALADDKKLKSIAFPSIGSGRNGFPKQTAAQLILKAISSYFVSTMSSSIKTVYFVLFDSESIGIYVQEMAKLDAN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75367 As Substrate

Site PTM Type Enzyme
K7 Acetylation
K12 Acetylation
K18 Acetylation
K18 Methylation
K18 Ubiquitination
R27 Methylation
Y55 Phosphorylation
K96 Ubiquitination
S102 Phosphorylation
K116 Ubiquitination
K117 Ubiquitination
S120 Phosphorylation
K121 Ubiquitination
K123 Acetylation
K123 Methylation
K123 Ubiquitination
T129 Phosphorylation
S138 Phosphorylation
S140 Phosphorylation
S146 Phosphorylation
K153 Acetylation
S158 Phosphorylation
K159 Acetylation
S166 Phosphorylation
K167 Ubiquitination
S170 Phosphorylation
S173 Phosphorylation
T174 Phosphorylation
T175 Phosphorylation
T178 Phosphorylation
T188 Phosphorylation
K189 Ubiquitination
K196 Ubiquitination
K235 Ubiquitination
K239 Methylation
R249 Methylation
K251 Ubiquitination
K271 Ubiquitination
S278 Phosphorylation
K285 Acetylation
K285 Ubiquitination
K292 Acetylation
K292 Ubiquitination
K295 Ubiquitination
K304 Acetylation
K304 Ubiquitination
K307 Ubiquitination
S308 Phosphorylation
S313 Phosphorylation
S316 Phosphorylation
K323 Ubiquitination
T325 Phosphorylation
K332 Ubiquitination
Y362 Phosphorylation

Research Backgrounds

Function:

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin.

Binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation. Increases the expression of genes involved in redox metabolism, including SOD3.

Represses SOD3 gene expression.

PTMs:

Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin.

Subcellular Location:

Nucleus. Chromosome.
Note: Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with HDAC1 and HDAC2 (By similarity). Interacts with SPOP. Part of a complex consisting of MACROH2A1, CUL3 and SPOP.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.