PRDM16 Antibody - #DF13303

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Product: PRDM16 Antibody
Catalog: DF13303
Description: Rabbit polyclonal antibody to PRDM16
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 70~140kDa; 140kD(Calculated).
Uniprot: Q9HAZ2
RRID: AB_2846322

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
PRDM16 Antibody detects endogenous levels of total PRDM16.
RRID:
AB_2846322
Cite Format: Affinity Biosciences Cat# DF13303, RRID:AB_2846322.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CMD1LL; KIAA1675; LVNC8; MDS1/EVI1 like gene 1; MDS1/EVI1-like gene 1; MEL1; PFM 13; PFM13; PR domain containing 16; PR domain containing protein 16; PR domain zinc finger protein 16; PR domain-containing protein 16; PRD16_HUMAN; Prdm16; Transcription factor MEL 1; Transcription factor MEL1;

Immunogens

Immunogen:
Uniprot:

Q9HAZ2(PRD16_HUMAN) >>Visit HPA database.

Gene(ID):
PRDM16(63976)
Expression:
Q9HAZ2 PRD16_HUMAN:

Expressed in uterus and kidney. Expressed in both cardiomyocytes and interstitial cells.

Sequence:
MRSKARARKLAKSDGDVVNNMYEPNRDLLASHSAEDEAEDSAMSPIPVGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPADFELRESSIPGAGLGVWAKRKMEAGERLGPCVVVPRAAAKETDFGWEQILTDVEVSPQEGCITKISEDLGSEKFCVDANQAGAGSWLKYIRVACSCDDQNLTMCQISEQIYYKVIKDIEPGEELLVHVKEGVYPLGTVPPGLDEEPTFRCDECDELFQSKLDLRRHKKYTCGSVGAALYEGLAEELKPEGLGGGSGQAHECKDCERMFPNKYSLEQHMVIHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHYTPGGIFAPGLPLTPSPMMDKAKPSPSLNHASLGFNEYFPSRPHPGSLPFSTAPPTFPALTPGFPGIFPPSLYPRPPLLPPTSLLKSPLNHTQDAKLPSPLGNPALPLVSAVSNSSQGTTAAAGPEEKFESRLEDSCVEKLKTRSSDMSDGSDFEDVNTTTGTDLDTTTGTGSDLDSDVDSDPDKDKGKGKSAEGQPKFGGGLAPPGAPNSVAEVPVFYSQHSFFPPPDEQLLTATGAAGDSIKAIASIAEKYFGPGFMGMQEKKLGSLPYHSAFPFQFLPNFPHSLYPFTDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKDVKPILPMPKGPSAPASGEEQPLDLSIGSRARASQNGGGREPRKNHVYGERKLGAGEGLPQVCPARMPQQPPLHYAKPSPFFMDPIYSRVEKRKVTDPVGALKEKYLRPSPLLFHPQMSAIETMTEKLESFAAMKADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKHEHENAPVSQHPGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQASTRTEKRADMQIVDGSAQCPGLASEKQEDVEEEDDDDLEEDDEDSLAGKSQDDTVSPAPEPQAAYEDEEDEEPAASLAVGFDHTRRCAEDHEGGLLALEPMPTFGKGLDLRRAAEEAFEVKDVLNSTLDSEALKHTLCRQAKNQAYAMMLSLSEDTPLHTPSQGSLDAWLKVTGATSESGAFHPINHL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Bovine
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HAZ2 As Substrate

Site PTM Type Enzyme
S44 Phosphorylation
S52 Phosphorylation
S66 Phosphorylation
Y193 Phosphorylation
Y194 Phosphorylation
K321 Sumoylation
S379 Phosphorylation
K382 Sumoylation
T421 Phosphorylation
S433 Phosphorylation
T434 Phosphorylation
T435 Phosphorylation
T464 Phosphorylation
S537 Phosphorylation
S581 Phosphorylation
S586 Phosphorylation
T611 Phosphorylation
K778 Ubiquitination
T876 Phosphorylation
K915 Acetylation
T974 Phosphorylation
S989 Phosphorylation
S1089 Phosphorylation
T1090 Phosphorylation
K1194 Acetylation

Research Backgrounds

Function:

Binds DNA and functions as a transcriptional regulator. Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (By similarity). Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation (By similarity). Likely to be one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation (By similarity). Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism (By similarity). Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells (By similarity). Functions as a repressor of TGF-beta signaling.

Binds DNA and functions as a transcriptional regulator. Functions as a repressor of TGF-beta signaling. May regulate granulocyte differentiation.

Subcellular Location:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in uterus and kidney. Expressed in both cardiomyocytes and interstitial cells.

Subunit Structure:

Interacts with CEBPA, CEBPB and CEBPD; the interaction is direct. Interacts with PPARG and PPARA; controls brown adipocytes differentiation. Interacts with CTBP1 and CTBP2; represses the expression of WAT-specific genes. Interacts with PPARGC1A and PPARGC1B; interaction with PPARGC1A or PPARGC1B activates the transcription of BAT-specific gene (By similarity). Interacts with HDAC1, SKI, SMAD2 and SMAD3; the interaction with SKI promotes the recruitment of SMAD3-HDAC1 complex on the promoter of TGF-beta target genes. Interacts with ZNF516; the interaction is direct and may play a role in the transcription of brown adipose tissue-specific gene.

Family&Domains:

Belongs to the PRDM16 family.

References

1). Cerasus humilis cherry polyphenol reduces high-fat diet-induced obesity in C57BL/6 mice by mitigating fat deposition, inflammation, and oxidation. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (PubMed: 32227855) [IF=6.1]

Application: WB    Species: mouse    Sample: white adipose

Figure 4. |Protein expression of genes related to antioxidant, adipogenic differentiation and beiging of the WAT in the retroperitoneal depot was detected by western-blot.(b) effect of CHP on lipid metabolism and adipocyte differentiation-related protein expression in obese mice induced by HFD

Application: WB    Species: mouse    Sample: white adipose

Figure 4. |Protein expression of genes related to antioxidant, adipogenic differentiation and beiging of the WAT in the retroperitoneal depot was detected by western-blot.(b) effect of CHP on lipid metabolism and adipocyte differentiation-related protein expression in obese mice induced by HFD; (c) CHP upregulation protein expression of white adipose browning gene in obese mice induced by HFD. Control group (CON): normal feed; high-fat diet group (HFD): highfat diet; high-fat diet feed supplemented orlistat (HFD + OST): HFD was fed for 12 weeks, orlistat was gavaged at 15.6 mg/kg bw/day, as a positive control group; CHP group supplemented with high-fat feed (HFD + CHP): HFD was fed for 12 weeks, CHP was gavaged 250 mg/kg bw/ day. Values are expressed as the mean ± standard deviation, n = 3. **p < 0.01, *p < 0.05.
2). Smilax china Polyphenols Stimulate Browning via 3-Adrenergic Receptor/AMP-Activated Protein Kinase Signaling Pathway in 3T3-L1 Adipocytes. The American journal of Chinese medicine (PubMed: 35642460) [IF=5.7]
3). Acacetin alleviates energy metabolism disorder through promoting white fat browning mediated by AC-cAMP pathway. Journal of Physiology and Biochemistry (PubMed: 36781604) [IF=3.4]
4). Prdm16-Mediated Browning is Involved in Resistance to Diet-Induced and Monosodium Glutamate-Induced Obesity. Diabetes Metabolic Syndrome and Obesity-Target & Therapy (PubMed: 34737591) [IF=3.3]

Application: WB    Species: Mice    Sample: adipose tissue

Figure 5 Prdm16 and Ucp-1 expression in subcutaneous WAT and comparison of serum leptin levels in each group. (A and B) H&E staining in the inguinal subcutaneous WAT of mice induced by DIO and MSG (scale bar = 110 μm). (C and D) Prdm16 expression in the adipose tissue of mice induced by DIO and MSG according to Western blot. (E and F) Ucp-1 expression in the adipose tissue of mice induced by DIO and MSG according to Western blot. (G) Immunoreactivity of Prdm16 in the adipose tissue of mice induced by a high-fat diet and presented as the mean ± SD (n = 3). #P < 0.05 vs the DIO group. (H) Immunoreactivity of Prdm16 in the adipose tissue of mice induced by MSG and presented as the mean ± SD (n = 3). #P < 0.05 vs the MSG group. (I) Immunoreactivity of Ucp-1 in the adipose tissue of mice induced by a high-fat diet and presented as the mean ± SD (n = 3). **P < 0.01 vs the NC group. ##P < 0.01 vs the DIO group. (J) Immunoreactivity of Ucp-1 in the adipose tissue of mice induced by MSG and presented as the mean ± SD (n = 3). **P < 0.01 vs the NC group. ###P < 0.001 vs the MSG group. Abbreviations: DIO, diet-induced obesity; MSG, mice with MSG-induced obesity; NC, normal control; Prdm16, PR domain containing 16; Ucp-1, uncoupling protein-1.

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