PGAM5 Antibody - #DF13355

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Product: PGAM5 Antibody
Catalog: DF13355
Description: Rabbit polyclonal antibody to PGAM5
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 32KD; 32kD(Calculated).
Uniprot: Q96HS1
RRID: AB_2846374

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
PGAM5 Antibody detects endogenous levels of total PGAM5.
RRID:
AB_2846374
Cite Format: Affinity Biosciences Cat# DF13355, RRID:AB_2846374.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Bcl-XL-binding protein v68; BXLBv68; MGC5352; mitochondrial; PGAM5; PGAM5_HUMAN; Phosphoglycerate mutase family member 5; Serine/threonine protein phosphatase PGAM5 mitochondrial; Serine/threonine-protein phosphatase PGAM5;

Immunogens

Immunogen:
Uniprot:

Q96HS1(PGAM5_HUMAN) >>Visit HPA database.

Gene(ID):
PGAM5(192111)
Sequence:
MAFRQALQLAACGLAGGSAAVLFSAVAVGKPRAGGDAEPRPAEPPAWAGGARPGPGVWDPNWDRREPLSLINVRKRNVESGEEELASKLDHYKAKATRHIFLIRHSQYHVDGSLEKDRTLTPLGREQAELTGLRLASLGLKFNKIVHSSMTRAIETTDIISRHLPGVCKVSTDLLREGAPIEPDPPVSHWKPEAVQYYEDGARIEAAFRNYIHRADARQEEDSYEIFICHANVIRYIVCRALQFPPEGWLRLSLNNGSITHLVIRPNGRVALRTLGDTGFMPPDKITRS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
75
Pig
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96HS1 As Substrate

Site PTM Type Enzyme
K75 Acetylation
K75 Ubiquitination
S80 Phosphorylation
S87 Phosphorylation
K88 Acetylation
K88 Ubiquitination
K93 Acetylation
K93 Ubiquitination
Y108 Phosphorylation
K116 Acetylation
T131 Phosphorylation
K141 Acetylation
K141 Ubiquitination
K144 Acetylation
K144 Ubiquitination
C168 S-Nitrosylation
K169 Ubiquitination
S171 Phosphorylation
K191 Acetylation
K191 Ubiquitination
S253 Phosphorylation
K285 Ubiquitination

Research Backgrounds

Function:

Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression. Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore.

PTMs:

Both isoform 1 and isoform 2 are phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions. This phosphorylation increases PGAM5 phosphatase activity.

Subcellular Location:

Mitochondrion outer membrane>Single-pass membrane protein.
Note: Isoform 2 overexpression results in the formation of disconnected punctuate mitochondria distributed throughout the cytoplasm. Isoform 1 overexpression results in the clustering of mitochondria around the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Dimer. Forms a ternary complex with NFE2L2 and KEAP1. Interacts with BCL2L1 and MAP3K5. Upon TNF-induced necrosis, forms in complex with RIPK1, RIPK3 and MLKL; the formation of this complex leads to PGAM5 phosphorylation. Isoform 2, but not isoform 1, interacts with DNM1L; this interaction leads to DNM1L dephosphorylation and activation and eventually to mitochondria fragmentation.

Family&Domains:

The N-terminal 35 amino acids, including the potential transmembrane alpha-helix, function as a non-cleaved mitochondrial targeting sequence that targets the protein to the cytosolic side of the outer mitochondrial membrane.

Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

References

1). Pterocephin A, a Novel Triterpenoid Saponin from Pterocephalus hookeri Induced Liver Injury by Activation of Necroptosis. PHYTOMEDICINE (PubMed: 33831690) [IF=7.9]

Application: WB    Species: Human    Sample: L-02 cells

Fig 4. PA activated RIP1 signaling pathway. A. Western blot analysis for RIP1 signaling pathway in L-02 cells with PA treatment; B. Western blot analysis for RIP1 signaling pathway with the interference of Nec-1 in L-02 cells. For A: Data were represented as mean ± SD (n = 3), * p < 0.05, ** p < 0.01 compared with the Con group. For B: Data were represented as mean ± SD (n = 3); # p < 0.05, ## p < 0.01 compared with the Con group; * p < 0.05, ** p < 0.01 compared with the PA- 4 group.
2). Vascular endothelial cell damage induced by ox-LDL through AMPK-Nrf2/Keap1-PGAM5 signaling axis. Research Square

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