GW182 Antibody - #DF13371

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Product: GW182 Antibody
Catalog: DF13371
Description: Rabbit polyclonal antibody to GW182
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 210kDa; 210kD(Calculated).
Uniprot: Q8NDV7
RRID: AB_2846390

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
GW182 Antibody detects endogenous levels of total GW182.
RRID:
AB_2846390
Cite Format: Affinity Biosciences Cat# DF13371, RRID:AB_2846390.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CAG repeat protein 26; CAGH26; DKFZp666E117; EDIE; EMSY interactor protein; FLJ22043; Glycine tryptophan protein of 182 kDa; Glycine-tryptophan protein of 182 kDa; GW1; GW182; GW182 autoantigen; Hypothetical protein DKFZp566M143; KIAA1460; MGC75384; OTTHUMP00000122485; OTTHUMP00000195164; OTTHUMP00000195165; Protein GW1; TNR6A_HUMAN; TNRC 6A; TNRC6; Tnrc6a; Trinucleotide repeat containing 6A; Trinucleotide repeat containing gene 6A protein; Trinucleotide repeat-containing gene 6A protein;

Immunogens

Immunogen:
Uniprot:

Q8NDV7(TNR6A_HUMAN) >>Visit HPA database.

Gene(ID):
TNRC6A(27327)
Expression:
Q8NDV7 TNR6A_HUMAN:

Ubiquitous.

Sequence:
MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NDV7 As Substrate

Site PTM Type Enzyme
S17 Phosphorylation
K146 Ubiquitination
S164 Phosphorylation
Y207 Phosphorylation
S210 Phosphorylation
S286 Phosphorylation
S333 Phosphorylation
S422 Phosphorylation
T426 Phosphorylation
S429 Phosphorylation
T603 Phosphorylation
S672 Phosphorylation
T726 Phosphorylation
T732 Phosphorylation
T736 Phosphorylation
T738 Phosphorylation
S739 Phosphorylation
S875 Phosphorylation
S878 Phosphorylation
S943 Phosphorylation
S991 Phosphorylation
Y1014 Phosphorylation
Y1016 Phosphorylation
T1044 Phosphorylation
S1175 Phosphorylation
K1176 Ubiquitination
S1209 Phosphorylation
S1212 Phosphorylation
S1214 Phosphorylation
S1217 Phosphorylation
S1224 Phosphorylation
S1230 Phosphorylation
K1237 Methylation
R1238 Methylation
S1245 Phosphorylation
Y1251 Phosphorylation
S1270 Phosphorylation
R1345 Methylation
S1356 Phosphorylation
S1357 Phosphorylation
S1372 Phosphorylation
S1431 Phosphorylation
S1434 Phosphorylation
S1457 Phosphorylation
T1470 Phosphorylation
K1484 Ubiquitination
S1503 Phosphorylation
S1509 Phosphorylation
S1533 Phosphorylation
S1545 Phosphorylation
T1548 Phosphorylation
S1549 Phosphorylation
S1554 Phosphorylation
S1555 Phosphorylation
T1581 Phosphorylation
S1582 Phosphorylation
S1585 Phosphorylation
S1599 Phosphorylation
Y1621 Phosphorylation
T1628 Phosphorylation
Y1631 Phosphorylation
S1660 Phosphorylation
S1661 Phosphorylation
T1666 Phosphorylation
S1704 Phosphorylation
K1741 Ubiquitination
S1750 Phosphorylation
S1761 Phosphorylation
K1786 Ubiquitination
K1826 Ubiquitination
T1867 Phosphorylation
S1869 Phosphorylation
S1884 Phosphorylation
S1888 Phosphorylation
S1890 Phosphorylation
S1934 Phosphorylation
S1942 Phosphorylation
S1944 Phosphorylation
S1961 Phosphorylation

Research Backgrounds

Function:

Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. As a scaffolding protein, associates with argonaute proteins bound to partially complementary mRNAs, and can simultaneously recruit CCR4-NOT and PAN deadenylase complexes.

Subcellular Location:

Cytoplasm>P-body.
Note: Mammalian P-bodies are also known as GW bodies (GWBs).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with AGO2. Interacts with AGO1, AGO3 and AGO4. Interacts with CNOT1; the interaction is direct and mediates the association with the CCR4-NOT complex. Interacts with ZC3H12A. Interacts with SND1.

Family&Domains:

Belongs to the GW182 family.

Restrictive clause

 

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