Spt6 Antibody - #DF13376
Product: | Spt6 Antibody |
Catalog: | DF13376 |
Description: | Rabbit polyclonal antibody to Spt6 |
Application: | WB IHC IF/ICC |
Reactivity: | Human, Mouse, Rat |
Prediction: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
Mol.Wt.: | 199kDa; 199kD(Calculated). |
Uniprot: | Q7KZ85 |
RRID: | AB_2846395 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13376, RRID:AB_2846395.
Fold/Unfold
emb 5; hSPT6; KIAA0162; MGC87943; SPT6H; SPT6H_HUMAN; Suppressor of Ty (S.cerevisiae) 6 homolog; Suppressor of Ty 6 homolog (S. cerevisiae); SUPT6H; Tat cotransactivator 2 protein; Tat CT2 protein; Tat-cotransactivator 2 protein; Tat-CT2 protein; Transcription elongation factor SPT6;
Immunogens
- Q7KZ85 SPT6H_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSDFVESEAEESEEEYNDEGEVVPRVTKKFVEEEDDDEEEEEENLDDQDEQGNLKGFINDDDDEDEGEEDEGSDSGDSEDDVGHKKRKRTSFDDRLEDDDFDLIEENLGVKVKRGQKYRRVKKMSDDEDDDEEEYGKEEHEKEAIAEEIFQDGEGEEGQEAMEAPMAPPEEEEEDDEESDIDDFIVDDDGQPLKKPKWRKKLPGYTDAALQEAQEIFGVDFDYDEFEKYNEYDEELEEEYEYEDDEAEGEIRVRPKKTTKKRVSRRSIFEMYEPSELESSHLTDQDNEIRATDLPERFQLRSIPVKGAEDDELEEEADWIYRNAFATPTISLQESCDYLDRGQPASSFSRKGPSTIQKIKEALGFMRNQHFEVPFIAFYRKEYVEPELHINDLWRVWQWDEKWTQLRIRKENLTRLFEKMQAYQYEQISADPDKPLADGIRALDTTDMERLKDVQSMDELKDVYNHFLLYYGRDIPKMQNAAKASRKKLKRVREEGDEEGEGDEAEDEEQRGPELKQASRRDMYTICQSAGLDGLAKKFGLTPEQFGENLRDSYQRHETEQFPAEPLELAKDYVCSQFPTPEAVLEGARYMVALQIAREPLVRQVLRQTFQERAKLNITPTKKGRKDVDEAHYAYSFKYLKNKPVKELRDDQFLKICLAEDEGLLTTDISIDLKGVEGYGNDQTYFEEIKQFYYRDEFSHQVQEWNRQRTMAIERALQQFLYVQMAKELKNKLLAEAKEYVIKACSRKLYNWLRVAPYRPDQQVEEDDDFMDENQGKGIRVLGIAFSSARDHPVFCALVNGEGEVTDFLRLPHFTKRRTAWREEEREKKAQDIETLKKFLLNKKPHVVTVAGENRDAQMLIEDVKRIVHELDQGQQLSSIGVELVDNELAILYMNSKKSEAEFRDYPPVLRQAVSLARRIQDPLIEFAQVCSSDEDILCLKFHPLQEHVVKEELLNALYCEFINRVNEVGVDVNRAIAHPYSQALIQYVCGLGPRKGTHLLKILKQNNTRLESRTQLVTMCHMGPKVFMNCAGFLKIDTASLGDSTDSYIEVLDGSRVHPETYEWARKMAVDALEYDESAEDANPAGALEEILENPERLKDLDLDAFAEELERQGYGDKHITLYDIRAELSCRYKDLRTAYRSPNTEEIFNMLTKETPETFYIGKLIICNVTGIAHRRPQGESYDQAIRNDETGLWQCPFCQQDNFPELSEVWNHFDSGSCPGQAIGVKTRLDNGVTGFIPTKFLSDKVVKRPEERVKVGMTVHCRIMKIDIEKFSADLTCRTSDLMDRNNEWKLPKDTYYDFDAEAADHKQEEDMKRKQQRTTYIKRVIAHPSFHNINFKQAEKMMETMDQGDVIIRPSSKGENHLTVTWKVSDGIYQHVDVREEGKENAFSLGATLWINSEEFEDLDEIVARYVQPMASFARDLLNHKYYQDCSGGDRKKLEELLIKTKKEKPTFIPYFICACKELPGKFLLGYQPRGKPRIEYVTVTPEGFRYRGQIFPTVNGLFRWFKDHYQDPVPGITPSSSSRTRTPASINATPANINLADLTRAVNALPQNMTSQMFSAIAAVTGQGQNPNATPAQWASSQYGYGGSGGGSSAYHVFPTPAQQPVATPLMTPSYSYTTPSQPITTPQYHQLQASTTPQSAQAQPQPSSSSRQRQQQPKSNSHAAIDWGKMAEQWLQEKEAERRKQKQRLTPRPSPSPMIESTPMSIAGDATPLLDEMDR
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q7KZ85 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S2 | Acetylation | Uniprot | |
S2 | Phosphorylation | Uniprot | |
S7 | Phosphorylation | Uniprot | |
S12 | Phosphorylation | Uniprot | |
K29 | Ubiquitination | Uniprot | |
S73 | Phosphorylation | Uniprot | |
S75 | Phosphorylation | Uniprot | |
S78 | Phosphorylation | Uniprot | |
T90 | Phosphorylation | Uniprot | |
S91 | Phosphorylation | Uniprot | |
R95 | Methylation | Uniprot | |
S125 | Phosphorylation | Uniprot | |
S179 | Phosphorylation | Uniprot | |
S267 | Phosphorylation | Uniprot | |
S279 | Phosphorylation | Uniprot | |
S280 | Phosphorylation | Uniprot | |
T283 | Phosphorylation | Uniprot | |
T292 | Phosphorylation | Uniprot | |
K351 | Ubiquitination | Uniprot | |
K358 | Ubiquitination | Uniprot | |
K360 | Ubiquitination | Uniprot | |
K402 | Acetylation | Uniprot | |
K402 | Ubiquitination | Uniprot | |
K419 | Ubiquitination | Uniprot | |
K434 | Ubiquitination | Uniprot | |
K452 | Ubiquitination | Uniprot | |
S456 | Phosphorylation | Uniprot | |
Y464 | Phosphorylation | Uniprot | |
Y470 | Phosphorylation | Uniprot | |
R511 | Methylation | Uniprot | |
K516 | Ubiquitination | Uniprot | |
K538 | Ubiquitination | Uniprot | |
K615 | Ubiquitination | Uniprot | |
T619 | Phosphorylation | Uniprot | |
T621 | Phosphorylation | Uniprot | |
Y685 | Phosphorylation | Uniprot | |
Y722 | Phosphorylation | Uniprot | |
K732 | Ubiquitination | Uniprot | |
K738 | Ubiquitination | Uniprot | |
Y740 | Phosphorylation | Uniprot | |
K743 | Acetylation | Uniprot | |
S746 | Phosphorylation | Uniprot | |
K777 | Ubiquitination | Uniprot | |
K816 | Ubiquitination | Uniprot | |
K844 | Ubiquitination | Uniprot | |
K1002 | Ubiquitination | Uniprot | |
K1005 | Ubiquitination | Uniprot | |
T1062 | Phosphorylation | Uniprot | |
Y1063 | Phosphorylation | Uniprot | |
Y1076 | Phosphorylation | Uniprot | |
S1079 | Phosphorylation | Uniprot | |
K1100 | Ubiquitination | Uniprot | |
T1122 | Phosphorylation | Uniprot | |
Y1124 | Phosphorylation | Uniprot | |
Y1134 | Phosphorylation | Uniprot | |
T1139 | Phosphorylation | Uniprot | |
T1172 | Phosphorylation | Uniprot | |
K1243 | Ubiquitination | Uniprot | |
K1248 | Ubiquitination | Uniprot | |
K1274 | Ubiquitination | Uniprot | |
R1289 | Methylation | Uniprot | |
K1294 | Ubiquitination | Uniprot | |
K1297 | Ubiquitination | Uniprot | |
K1341 | Ubiquitination | Uniprot | |
Y1378 | Phosphorylation | Uniprot | |
T1450 | Phosphorylation | Uniprot | |
K1471 | Ubiquitination | Uniprot | |
Y1486 | Phosphorylation | Uniprot | |
K1512 | Ubiquitination | Uniprot | |
Y1515 | Phosphorylation | Uniprot | |
T1523 | Phosphorylation | P24941 (CDK2) | Uniprot |
S1525 | Phosphorylation | Uniprot | |
S1526 | Phosphorylation | Uniprot | |
S1527 | Phosphorylation | Uniprot | |
S1528 | Phosphorylation | Uniprot | |
T1530 | Phosphorylation | Uniprot | |
T1532 | Phosphorylation | Uniprot | |
S1535 | Phosphorylation | Uniprot | |
T1539 | Phosphorylation | Uniprot | |
S1641 | Phosphorylation | Uniprot | |
S1666 | Phosphorylation | Uniprot | |
K1676 | Acetylation | Uniprot | |
K1676 | Ubiquitination | Uniprot | |
T1697 | Phosphorylation | Uniprot | |
S1701 | Phosphorylation | Uniprot | |
S1703 | Phosphorylation | Uniprot | |
T1718 | Phosphorylation | Uniprot |
Research Backgrounds
Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.
Nucleus.
Ubiquitously expressed.
Interacts with RNA polymerase II and the DRB sensitivity-inducing factor complex (DSIF complex), which is composed of SUPT5H and SUPT4H1. Interacts with KDM6A (By similarity). Interacts (via SH2 domain) with SETD1A (By similarity). Interacts (via SH2 domain) with POLR2A phosphorylated at 'Ser-2'. Interacts with IWS1, AICDA and PAAF1. Interacts with histone H2B and H3. Interacts with WDR43 (By similarity).
(Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein UL69.
Belongs to the SPT6 family.
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