Product: Spt6 Antibody
Catalog: DF13376
Description: Rabbit polyclonal antibody to Spt6
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 199kDa; 199kD(Calculated).
Uniprot: Q7KZ85
RRID: AB_2846395

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Spt6 Antibody detects endogenous levels of total Spt6.
RRID:
AB_2846395
Cite Format: Affinity Biosciences Cat# DF13376, RRID:AB_2846395.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

emb 5; hSPT6; KIAA0162; MGC87943; SPT6H; SPT6H_HUMAN; Suppressor of Ty (S.cerevisiae) 6 homolog; Suppressor of Ty 6 homolog (S. cerevisiae); SUPT6H; Tat cotransactivator 2 protein; Tat CT2 protein; Tat-cotransactivator 2 protein; Tat-CT2 protein; Transcription elongation factor SPT6;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q7KZ85 SPT6H_HUMAN:

Ubiquitously expressed.

Sequence:
MSDFVESEAEESEEEYNDEGEVVPRVTKKFVEEEDDDEEEEEENLDDQDEQGNLKGFINDDDDEDEGEEDEGSDSGDSEDDVGHKKRKRTSFDDRLEDDDFDLIEENLGVKVKRGQKYRRVKKMSDDEDDDEEEYGKEEHEKEAIAEEIFQDGEGEEGQEAMEAPMAPPEEEEEDDEESDIDDFIVDDDGQPLKKPKWRKKLPGYTDAALQEAQEIFGVDFDYDEFEKYNEYDEELEEEYEYEDDEAEGEIRVRPKKTTKKRVSRRSIFEMYEPSELESSHLTDQDNEIRATDLPERFQLRSIPVKGAEDDELEEEADWIYRNAFATPTISLQESCDYLDRGQPASSFSRKGPSTIQKIKEALGFMRNQHFEVPFIAFYRKEYVEPELHINDLWRVWQWDEKWTQLRIRKENLTRLFEKMQAYQYEQISADPDKPLADGIRALDTTDMERLKDVQSMDELKDVYNHFLLYYGRDIPKMQNAAKASRKKLKRVREEGDEEGEGDEAEDEEQRGPELKQASRRDMYTICQSAGLDGLAKKFGLTPEQFGENLRDSYQRHETEQFPAEPLELAKDYVCSQFPTPEAVLEGARYMVALQIAREPLVRQVLRQTFQERAKLNITPTKKGRKDVDEAHYAYSFKYLKNKPVKELRDDQFLKICLAEDEGLLTTDISIDLKGVEGYGNDQTYFEEIKQFYYRDEFSHQVQEWNRQRTMAIERALQQFLYVQMAKELKNKLLAEAKEYVIKACSRKLYNWLRVAPYRPDQQVEEDDDFMDENQGKGIRVLGIAFSSARDHPVFCALVNGEGEVTDFLRLPHFTKRRTAWREEEREKKAQDIETLKKFLLNKKPHVVTVAGENRDAQMLIEDVKRIVHELDQGQQLSSIGVELVDNELAILYMNSKKSEAEFRDYPPVLRQAVSLARRIQDPLIEFAQVCSSDEDILCLKFHPLQEHVVKEELLNALYCEFINRVNEVGVDVNRAIAHPYSQALIQYVCGLGPRKGTHLLKILKQNNTRLESRTQLVTMCHMGPKVFMNCAGFLKIDTASLGDSTDSYIEVLDGSRVHPETYEWARKMAVDALEYDESAEDANPAGALEEILENPERLKDLDLDAFAEELERQGYGDKHITLYDIRAELSCRYKDLRTAYRSPNTEEIFNMLTKETPETFYIGKLIICNVTGIAHRRPQGESYDQAIRNDETGLWQCPFCQQDNFPELSEVWNHFDSGSCPGQAIGVKTRLDNGVTGFIPTKFLSDKVVKRPEERVKVGMTVHCRIMKIDIEKFSADLTCRTSDLMDRNNEWKLPKDTYYDFDAEAADHKQEEDMKRKQQRTTYIKRVIAHPSFHNINFKQAEKMMETMDQGDVIIRPSSKGENHLTVTWKVSDGIYQHVDVREEGKENAFSLGATLWINSEEFEDLDEIVARYVQPMASFARDLLNHKYYQDCSGGDRKKLEELLIKTKKEKPTFIPYFICACKELPGKFLLGYQPRGKPRIEYVTVTPEGFRYRGQIFPTVNGLFRWFKDHYQDPVPGITPSSSSRTRTPASINATPANINLADLTRAVNALPQNMTSQMFSAIAAVTGQGQNPNATPAQWASSQYGYGGSGGGSSAYHVFPTPAQQPVATPLMTPSYSYTTPSQPITTPQYHQLQASTTPQSAQAQPQPSSSSRQRQQQPKSNSHAAIDWGKMAEQWLQEKEAERRKQKQRLTPRPSPSPMIESTPMSIAGDATPLLDEMDR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7KZ85 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S7 Phosphorylation
S12 Phosphorylation
K29 Ubiquitination
S73 Phosphorylation
S75 Phosphorylation
S78 Phosphorylation
T90 Phosphorylation
S91 Phosphorylation
R95 Methylation
S125 Phosphorylation
S179 Phosphorylation
S267 Phosphorylation
S279 Phosphorylation
S280 Phosphorylation
T283 Phosphorylation
T292 Phosphorylation
K351 Ubiquitination
K358 Ubiquitination
K360 Ubiquitination
K402 Acetylation
K402 Ubiquitination
K419 Ubiquitination
K434 Ubiquitination
K452 Ubiquitination
S456 Phosphorylation
Y464 Phosphorylation
Y470 Phosphorylation
R511 Methylation
K516 Ubiquitination
K538 Ubiquitination
K615 Ubiquitination
T619 Phosphorylation
T621 Phosphorylation
Y685 Phosphorylation
Y722 Phosphorylation
K732 Ubiquitination
K738 Ubiquitination
Y740 Phosphorylation
K743 Acetylation
S746 Phosphorylation
K777 Ubiquitination
K816 Ubiquitination
K844 Ubiquitination
K1002 Ubiquitination
K1005 Ubiquitination
T1062 Phosphorylation
Y1063 Phosphorylation
Y1076 Phosphorylation
S1079 Phosphorylation
K1100 Ubiquitination
T1122 Phosphorylation
Y1124 Phosphorylation
Y1134 Phosphorylation
T1139 Phosphorylation
T1172 Phosphorylation
K1243 Ubiquitination
K1248 Ubiquitination
K1274 Ubiquitination
R1289 Methylation
K1294 Ubiquitination
K1297 Ubiquitination
K1341 Ubiquitination
Y1378 Phosphorylation
T1450 Phosphorylation
K1471 Ubiquitination
Y1486 Phosphorylation
K1512 Ubiquitination
Y1515 Phosphorylation
T1523 Phosphorylation P24941 (CDK2)
S1525 Phosphorylation
S1526 Phosphorylation
S1527 Phosphorylation
S1528 Phosphorylation
T1530 Phosphorylation
T1532 Phosphorylation
S1535 Phosphorylation
T1539 Phosphorylation
S1641 Phosphorylation
S1666 Phosphorylation
K1676 Acetylation
K1676 Ubiquitination
T1697 Phosphorylation
S1701 Phosphorylation
S1703 Phosphorylation
T1718 Phosphorylation

Research Backgrounds

Function:

Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Interacts with RNA polymerase II and the DRB sensitivity-inducing factor complex (DSIF complex), which is composed of SUPT5H and SUPT4H1. Interacts with KDM6A (By similarity). Interacts (via SH2 domain) with SETD1A (By similarity). Interacts (via SH2 domain) with POLR2A phosphorylated at 'Ser-2'. Interacts with IWS1, AICDA and PAAF1. Interacts with histone H2B and H3. Interacts with WDR43 (By similarity).

(Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein UL69.

Family&Domains:

Belongs to the SPT6 family.

Restrictive clause

 

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