Product: DAP3 Antibody
Catalog: DF13390
Description: Rabbit polyclonal antibody to DAP3
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 45kDa; 46kD(Calculated).
Uniprot: P51398
RRID: AB_2846409

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
DAP3 Antibody detects endogenous levels of total DAP3.
RRID:
AB_2846409
Cite Format: Affinity Biosciences Cat# DF13390, RRID:AB_2846409.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

28S ribosomal protein S29; 28S ribosomal protein S29 mitochondrial; bMRP10; DAP 3; DAP-3; Dap3; Death associated protein 3; Death-associated protein 3; DKFZp686G12159; FLJ12817; Ionizing radiation resistance conferring protein; MGC126058; MGC126059; Mitochondrial 28S ribosomal protein S29; mitochondrial; MITOCHONDRIAL RIBOSOMAL PROTEIN S29; MRP S29; MRP-S29; MRPS29; OTTHUMP00000033480; OTTHUMP00000033481; PSD-95/SAP90-binding protein 3; RT29_HUMAN; S29mt; SAP90/PSD-95-associated protein 3; SAPAP3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P51398 RT29_HUMAN:

Ubiquitous.

Sequence:
MMLKGITRLISRIHKLDPGRFLHMGTQARQSIAAHLDNQVPVESPRAISRTNENDPAKHGDQHEGQHYNISPQDLETVFPHGLPPRFVMQVKTFSEACLMVRKPALELLHYLKNTSFAYPAIRYLLYGEKGTGKTLSLCHVIHFCAKQDWLILHIPDAHLWVKNCRDLLQSSYNKQRFDQPLEASTWLKNFKTTNERFLNQIKVQEKYVWNKRESTEKGSPLGEVVEQGITRVRNATDAVGIVLKELKRQSSLGMFHLLVAVDGINALWGRTTLKREDKSPIAPEELALVHNLRKMMKNDWHGGAIVSALSQTGSLFKPRKAYLPQELLGKEGFDALDPFIPILVSNYNPKEFESCIQYYLENNWLQHEKAPTEEGKKELLFLSNANPSLLERHCAYL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
88
Rabbit
88
Chicken
75
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51398 As Substrate

Site PTM Type Enzyme
S31 Phosphorylation Q05655 (PRKCD) , P17612 (PRKACA)
S44 Phosphorylation
T93 Phosphorylation
K103 Ubiquitination
Y124 Phosphorylation
K130 Ubiquitination
K175 Acetylation
K175 Ubiquitination
S185 Phosphorylation Q05655 (PRKCD) , P17612 (PRKACA)
T186 Phosphorylation Q05655 (PRKCD) , P17612 (PRKACA)
K189 Ubiquitination
K192 Ubiquitination
K203 Ubiquitination
K207 Acetylation
K207 Ubiquitination
S215 Phosphorylation
T216 Phosphorylation
K218 Ubiquitination
S220 Phosphorylation P17612 (PRKACA)
T231 Phosphorylation
T237 Phosphorylation Q05655 (PRKCD)
K245 Ubiquitination
S251 Phosphorylation
S252 Phosphorylation
T272 Phosphorylation
T273 Phosphorylation
K279 Ubiquitination
S280 Phosphorylation Q05655 (PRKCD)
S311 Phosphorylation
K318 Ubiquitination
K321 Ubiquitination
K370 Ubiquitination
K378 Ubiquitination
S389 Phosphorylation

Research Backgrounds

Function:

Involved in mediating interferon-gamma-induced cell death.

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Component of the mitochondrial small ribosomal subunit (mt-SSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins. Interacts with DELE1. Interacts with NOA1.

Family&Domains:

Belongs to the mitochondrion-specific ribosomal protein mS29 family.

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