CBX4 Antibody - #DF13461

(3)  
Product: CBX4 Antibody
Catalog: DF13461
Description: Rabbit polyclonal antibody to CBX4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 61kDa; 61kD(Calculated).
Uniprot: O00257
RRID: AB_2846480

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Zebrafish(88%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
CBX4 Antibody detects endogenous levels of total CBX4.
RRID:
AB_2846480
Cite Format: Affinity Biosciences Cat# DF13461, RRID:AB_2846480.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CBX 4; CBX4; Cbx4 chromobox homolog 4 (Drosophila Pc class); CBX4_HUMAN; Chromobox homolog 4 (Pc class homolog, Drosophila); Chromobox homolog 4; Chromobox protein homolog 4; E3 SUMO protein ligase CBX 4; E3 SUMO protein ligase CBX4; E3 SUMO-protein ligase CBX4; hPc 2; hPc2; NBP 16; NBP16; NS5ATP1 binding protein 16; Pc 2; Pc class 2 homolog; Pc class homolog; Pc class homolog Drosophila; PC2; Polycomb 2 homolog;

Immunogens

Immunogen:
Uniprot:

O00257(CBX4_HUMAN) >>Visit HPA database.

Gene(ID):
CBX4(8535)
Expression:
O00257 CBX4_HUMAN:

Ubiquitous.

Sequence:
MELPAVGEHVFAVESIEKKRIRKGRVEYLVKWRGWSPKYNTWEPEENILDPRLLIAFQNRERQEQLMGYRKRGPKPKPLVVQVPTFARRSNVLTGLQDSSTDNRAKLDLGAQGKGQGHQYELNSKKHHQYQPHSKERAGKPPPPGKSGKYYYQLNSKKHHPYQPDPKMYDLQYQGGHKEAPSPTCPDLGAKSHPPDKWAQGAGAKGYLGAVKPLAGAAGAPGKGSEKGPPNGMMPAPKEAVTGNGIGGKMKIVKNKNKNGRIVIVMSKYMENGMQAVKIKSGEVAEGEARSPSHKKRAADERHPPADRTFKKAAGAEEKKVEAPPKRREEEVSGVSDPQPQDAGSRKLSPTKEAFGEQPLQLTTKPDLLAWDPARNTHPPSHHPHPHPHHHHHHHHHHHHAVGLNLSHVRKRCLSETHGEREPCKKRLTARSISTPTCLGGSPAAERPADLPPAAALPQPEVILLDSDLDEPIDLRCVKTRSEAGEPPSSLQVKPETPASAAVAVAAAAAPTTTAEKPPAEAQDEPAESLSEFKPFFGNIIITDVTANCLTVTFKEYVTV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
88
Zebrafish
88
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00257 As Substrate

Site PTM Type Enzyme
Y28 Phosphorylation
K38 Ubiquitination
S90 Phosphorylation
K106 Sumoylation
K106 Ubiquitination
K114 Sumoylation
K114 Ubiquitination
Y120 Phosphorylation
K125 Ubiquitination
R137 Methylation
K149 Acetylation
K149 Sumoylation
K149 Ubiquitination
Y150 Phosphorylation
Y151 Phosphorylation
Y152 Phosphorylation
K157 Acetylation
K158 Ubiquitination
K167 Ubiquitination
Y169 Phosphorylation
K178 Ubiquitination
S182 Phosphorylation
T184 Phosphorylation
K197 Ubiquitination
K205 Sumoylation
K205 Ubiquitination
K212 Sumoylation
K212 Ubiquitination
K223 Sumoylation
K223 Ubiquitination
K249 Methylation
K249 Sumoylation
K278 Sumoylation
K278 Ubiquitination
K280 Sumoylation
K280 Ubiquitination
S291 Phosphorylation
S293 Phosphorylation
S336 Phosphorylation
S345 Phosphorylation
S349 Phosphorylation
K365 Sumoylation
K365 Ubiquitination
S415 Phosphorylation
T417 Phosphorylation
S432 Phosphorylation
S434 Phosphorylation
T437 Phosphorylation
S442 Phosphorylation
S467 Phosphorylation
K494 Sumoylation
T497 Phosphorylation Q9H2X6 (HIPK2)

Research Backgrounds

Function:

E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131.

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3) (By similarity). Plays a role in the lineage differentiation of the germ layers in embryonic development (By similarity).

PTMs:

Phosphorylated on Thr-497 by HIPK2 upon DNA damage. This phosphorylation stimulates E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494, as well as sumoylation of other target proteins, such as HNRNPK.

Subcellular Location:

Nucleus. Nucleus speckle.
Note: Localization to nuclear polycomb bodies is required for ZNF131 sumoylation (PubMed:22467880). Localized in distinct foci on chromatin (PubMed:18927235).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with histone H3-K9Me3 (By similarity). Interacts with CHTOP (By similarity). Component of a PRC1-like complex. The composition of the PRC1 complex differs between the PRC1 complex in pluripotent embryonic stem cells containing RNF2, CBX7 and PCGF2, and the PRC1 complex in differentiating cells containing RNF2, CBX2, CBX4 and BMI1 (By similarity). Self-associates. Interacts with SUV39H1 and HIPK2. Interacts with CSNK2B. May interact with HIST2H3A, HIST1H3A and RNF2. Interacts with SUMO1P1/SUMO5.

Family&Domains:

The polyhistidine repeat may act as a targeting signal to nuclear speckles.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.