Product: ACTR1A Antibody
Catalog: DF13535
Description: Rabbit polyclonal antibody to ACTR1A
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 42kDa; 43kD(Calculated).
Uniprot: P61163
RRID: AB_2846554

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ACTR1A Antibody detects endogenous levels of total ACTR1A.
RRID:
AB_2846554
Cite Format: Affinity Biosciences Cat# DF13535, RRID:AB_2846554.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Actin related protein 1; Actin RPV; Actin-RPV; ACTR 1A; Actr1a; ACTZ_HUMAN; Alpha centractin; Alpha-centractin; ARP 1; ARP1 actin related protein 1 homolog A; ARP1 actin related protein 1 homolog A centractin alpha; ARP1; ARP1 yeast homolog A; Centractin alpha; Centractin; Centrosome associated actin homolog; Centrosome-associated actin homolog; CTRN 1; CTRN1; FLJ52695; FLJ52800; FLJ55002;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MESYDVIANQPVVIDNGSGVIKAGFAGDQIPKYCFPNYVGRPKHVRVMAGALEGDIFIGPKAEEHRGLLSIRYPMEHGIVKDWNDMERIWQYVYSKDQLQTFSEEHPVLLTEAPLNPRKNRERAAEVFFETFNVPALFISMQAVLSLYATGRTTGVVLDSGDGVTHAVPIYEGFAMPHSIMRIDIAGRDVSRFLRLYLRKEGYDFHSSSEFEIVKAIKERACYLSINPQKDETLETEKAQYYLPDGSTIEIGPSRFRAPELLFRPDLIGEESEGIHEVLVFAIQKSDMDLRRTLFSNIVLSGGSTLFKGFGDRLLSEVKKLAPKDVKIRISAPQERLYSTWIGGSILASLDTFKKMWVSKKEYEEDGARSIHRKTF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
75
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61163 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S3 Phosphorylation
Y4 Phosphorylation
K22 Ubiquitination
K32 Acetylation
K32 Ubiquitination
C34 S-Nitrosylation
K43 Ubiquitination
R66 Methylation
R72 Methylation
K81 Ubiquitination
K96 Ubiquitination
Y171 Phosphorylation
K200 Ubiquitination
K230 Acetylation
K230 Ubiquitination
K238 Ubiquitination
S301 Phosphorylation
K308 Ubiquitination
R313 Methylation
K319 Ubiquitination
S331 Phosphorylation
Y338 Phosphorylation

Research Backgrounds

Function:

Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cell cortex.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with BCCIP (isoform 2/alpha).

Family&Domains:

Belongs to the actin family. ARP1 subfamily.

Restrictive clause

 

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For Research Use Only.
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