TEA domain family member 2 Antibody

1/2

Western blot analysis of extracts from Human Fetal brain, using TEA domain family member 2 Antibody.

DF13565 at 1/100 staining Rat testis tissue by IHC-P.

Product:	TEA domain family member 2 Antibody
Catalog:	DF13565
Description:	Rabbit polyclonal antibody to TEA domain family member 2
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.:	49kDa; 49kD(Calculated).
Uniprot:	Q15562
RRID:	AB_2846584

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(88%), Bovine(86%), Horse(88%), Rabbit(100%), Dog(86%)

Clonality:

Polyclonal

Specificity:

TEA domain family member 2 Antibody detects endogenous levels of total TEA domain family member 2.

RRID:

AB_2846584
Cite Format: Affinity Biosciences Cat# DF13565, RRID:AB_2846584.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Embryonic TEA domain containing factor; Etdf; ETEF 1; ETF; TEA domain family member 2; TEAD 2; TEAD-2; TEAD2; Tead2 protein (TEA domain family member 2 (Mapped), isoform CRA_b); TEAD2_HUMAN; TEF 4; TEF4; Transcriptional enhancer factor 4; Transcriptional enhancer factor TEF 4; Transcriptional enhancer factor TEF-4;

Immunogens

Immunogen:

Uniprot:

Q15562(TEAD2_HUMAN) >>Visit HPA database.

Gene(ID):

TEAD2(8463)

Sequence:

Q15562 TEAD2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGEPRAGAALDDGSGWTGSEEGSEEGTGGSEGAGGDGGPDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARRKSREIQSKLKDQVSKDKAFQTMATMSSAQLISAPSLQAKLGPTGPQASELFQFWSGGSGPPWNVPDVKPFSQTPFTLSLTPPSTDLPGYEPPQALSPLPPPTPSPPAWQARGLGTARLQLVEFSAFVEPPDAVDSYQRHLFVHISQHCPSPGAPPLESVDVRQIYDKFPEKKGGLRELYDRGPPHAFFLVKFWADLNWGPSGEEAGAGGSISSGGFYGVSSQYESLEHMTLTCSSKVCSFGKQVVEKVETERAQLEDGRFVYRLLRSPMCEYLVNFLHKLRQLPERYMMNSVLENFTILQVVTNRDTQELLLCTAYVFEVSTSERGAQHHIYRLVRD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Bovine

Dog

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15562 As Substrate

Q15562

Site	PTM Type	Source
K67	Ubiquitination	Uniprot
S71	Phosphorylation	Uniprot
K75	Ubiquitination	Uniprot
Y86	Phosphorylation	Uniprot
K93	Methylation	Uniprot
K98	Ubiquitination	Uniprot
S112	Phosphorylation	Uniprot
S117	Phosphorylation	Uniprot
K125	Ubiquitination	Uniprot
K127	Ubiquitination	Uniprot
T131	Phosphorylation	Uniprot
S260	Phosphorylation	Uniprot
Y275	Phosphorylation	Uniprot
K277	Ubiquitination	Uniprot
K352	Ubiquitination	Uniprot

Research Backgrounds

Q15562_TEAD2_HUMAN

Function:

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.

Subcellular Location:

Nucleus.

Subunit Structure:

Interacts with YAP1 and WWTR1/TAZ.

Research Fields

· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species. (View pathway)

Restrictive clause

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TEA domain family member 2 Antibody - #DF13565