KLF2 Antibody | Affinity Biosciences

Product:	KLF2 Antibody
Catalog:	DF13602
Description:	Rabbit polyclonal antibody to KLF2
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Chicken, Xenopus
Mol.Wt.:	37kD, 52kD; 37kD(Calculated).
Uniprot:	Q9Y5W3
RRID:	AB_2846621

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Chicken(100%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

KLF2 Antibody detects endogenous levels of total KLF2.

RRID:

AB_2846621
Cite Format: Affinity Biosciences Cat# DF13602, RRID:AB_2846621.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

KLF 2; Klf2; KLF2_HUMAN; Krueppel-like factor 2; Kruppel like factor 2; Kruppel like factor; Kruppel like factor LKLF; Kruppel-like factor 2 (lung); Lklf; Lung krueppel like factor; Lung krueppel-like factor; Lung Kruppel like zinc finger transcription factor;

Immunogens

Immunogen:

Uniprot:

Q9Y5W3(KLF2_HUMAN) >>Visit HPA database.

Gene(ID):

KLF2(10365)

Sequence:

Q9Y5W3 KLF2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALSEPILPSFSTFASPCRERGLQERWPRAEPESGGTDDDLNSVLDFILSMGLDGLGAEAAPEPPPPPPPPAFYYPEPGAPPPYSAPAGGLVSELLRPELDAPLGPALHGRFLLAPPGRLVKAEPPEADGGGGYGCAPGLTRGPRGLKREGAPGPAASCMRGPGGRPPPPPDTPPLSPDGPARLPAPGPRASFPPPFGGPGFGAPGPGLHYAPPAPPAFGLFDDAAAAAAALGLAPPAARGLLTPPASPLELLEAKPKRGRRSWPRKRTATHTCSYAGCGKTYTKSSHLKAHLRTHTGEKPYHCNWDGCGWKFARSDELTRHYRKHTGHRPFQCHLCDRAFSRSDHLALHMKRHM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Chicken

100

Xenopus

Pig

Horse

Bovine

Sheep

Dog

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y5W3 As Substrate

Q9Y5W3

Site	PTM Type	Source
S16	Phosphorylation	Uniprot
K122	Sumoylation	Uniprot
K148	Sumoylation	Uniprot
T173	Phosphorylation	Uniprot
S177	Phosphorylation	Uniprot
T244	Phosphorylation	Uniprot
S248	Phosphorylation	Uniprot
S286	Phosphorylation	Uniprot
S287	Phosphorylation	Uniprot
K290	Sumoylation	Uniprot
K290	Ubiquitination	Uniprot
T295	Phosphorylation	Uniprot
T297	Phosphorylation	Uniprot
T327	Phosphorylation	Uniprot

Research Backgrounds

Q9Y5W3_KLF2_HUMAN

Function:

Transcription factor that binds to the CACCC box in the promoter of target genes such as HBB/beta globin or NOV and activates their transcription. Might be involved in transcriptional regulation by modulating the binding of the RARA nuclear receptor to RARE DNA elements.

PTMs:

Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein (By similarity).

Subcellular Location:

Nucleus.

Subunit Structure:

Interacts with WWP1.

Family&Domains:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the krueppel C2H2-type zinc-finger protein family.

Research Fields

· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)

References

1). Bioinformatics analysis of KLF2 as a potential prognostic factor in ccRCC and association with epithelial‑mesenchymal transition. Experimental and Therapeutic Medicine (PubMed: 35978925) [IF=2.7]

Application: WB Species: Human Sample: HK-2 cells

Figure 3 KLF2 inhibits ccRCC migration in vitro and KLF2 protein expression is decreased in high pathological grade tissue (II + III). (A) Expression of KLF2 mRNA in ccRCC and normal HK-2 cells. (B) Western blotting was used for assessment of KLF2 protein levels in ccRCC and normal HK-2 cells. (C) Western blotting assessment of KLF2 OE in 769-P and 786-O cells. (D) Migration of 769-P and 786-O cells after wounding (magnification, x40). (E) compared with NC. (F) Immunohistochemistry and (G) analysis of the KLF2 expression in ccRCC tissue (n=20). **P<0.01 vs. stage I and ***P<0.001 vs. NC. KLF, Krüppel-like factor; ccRCC, clear cell renal cell carcinoma; NC, negative control; OE, overexpression.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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KLF2 Antibody - #DF13602