Phospho-Dicer (Tyr1438) Antibody | Affinity Biosciences

Product:	Phospho-Dicer (Tyr1438) Antibody
Catalog:	AF3513
Description:	Rabbit polyclonal antibody to Phospho-Dicer (Tyr1438)
Application:	ELISA(peptide)
Reactivity:	Human
Mol.Wt.:	219kD(Calculated).
Uniprot:	Q9UPY3
RRID:	AB_2846827

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Phospho-Dicer (Tyr1438) Antibody detects endogenous levels of Dicer only when phosphorylated at Tyr1438.

RRID:

AB_2846827
Cite Format: Affinity Biosciences Cat# AF3513, RRID:AB_2846827.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DCR; DCR1; Dicer 1 ribonuclease III; Dicer 1 ribonuclease type III; Dicer; DICER_HUMAN; DICER1; Double-strand-specific ribonuclease; Endoribonuclease Dicer; Helicase MOI; Helicase with RNase motif; HERNA; KIAA0928; MNG1; RMSE2;

Immunogens

Immunogen:

A synthesized peptide derived from human Dicer around the phosphorylation site of Tyr1438.

Uniprot:

Q9UPY3(DICER_HUMAN) >>Visit HPA database.

Gene(ID):

DICER1(23405)

Sequence:

Q9UPY3 DICER_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

PTMs - Q9UPY3 As Substrate

Q9UPY3

Site	PTM Type	Source
S82	Phosphorylation	Uniprot
T238	Phosphorylation	Uniprot
S397	Phosphorylation	Uniprot
S413	Phosphorylation	Uniprot
S415	Phosphorylation	Uniprot
Y452	Phosphorylation	Uniprot
K466	Ubiquitination	Uniprot
K570	Ubiquitination	Uniprot
K577	Ubiquitination	Uniprot
S643	Phosphorylation	Uniprot
K652	Ubiquitination	Uniprot
Y664	Phosphorylation	Uniprot
Y668	Phosphorylation	Uniprot
Y819	Phosphorylation	Uniprot
T820	Phosphorylation	Uniprot
S828	Phosphorylation	Uniprot
S962	Phosphorylation	Uniprot
T967	Phosphorylation	Uniprot
T1001	Phosphorylation	Uniprot
S1016	Phosphorylation	Uniprot
K1023	Ubiquitination	Uniprot
Y1091	Phosphorylation	Uniprot
Y1121	Phosphorylation	Uniprot
C1122	S-Nitrosylation	Uniprot
S1142	Phosphorylation	Uniprot
S1160	Phosphorylation	Uniprot
Y1204	Phosphorylation	Uniprot
S1252	Phosphorylation	Uniprot
S1255	Phosphorylation	Uniprot
S1280	Phosphorylation	Uniprot
T1329	Phosphorylation	Uniprot
Y1330	Phosphorylation	Uniprot
K1350	Ubiquitination	Uniprot
K1391	Acetylation	Uniprot
K1433	Ubiquitination	Uniprot
Y1438	Phosphorylation	Uniprot
Y1445	Phosphorylation	Uniprot
S1460	Phosphorylation	Uniprot
K1465	Ubiquitination	Uniprot
K1466	Ubiquitination	Uniprot
S1468	Phosphorylation	Uniprot
S1470	Phosphorylation	Uniprot
Y1479	Phosphorylation	Uniprot
K1595	Ubiquitination	Uniprot
S1612	Phosphorylation	Uniprot
K1615	Ubiquitination	Uniprot
S1728	Phosphorylation	Uniprot
S1852	Phosphorylation	Uniprot
S1868	Phosphorylation	Uniprot
S1922	Phosphorylation	Uniprot

Research Backgrounds

Q9UPY3_DICER_HUMAN

Function:

Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.

Subcellular Location:

Cytoplasm.

Subunit Structure:

Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2; DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with DHX9, AGO1, PIWIL1 and PRKRA. Associates with the 60S ribosome. Interacts with BCDIN3D. Interacts with AGO2, TARBP2, EIF6, MOV10 and RPL7A (60S ribosome subunit); they form a large RNA-induced silencing complex (RISC).

Family&Domains:

Belongs to the helicase family. Dicer subfamily.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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Phospho-Dicer (Tyr1438) Antibody - #AF3513