Phospho-MLKL (Thr357/Ser358) Antibody

Product:	Phospho-MLKL (Thr357/Ser358) Antibody
Catalog:	AF3892
Description:	Rabbit polyclonal antibody to Phospho-MLKL (Thr357/Ser358)
Application:	ELISA(peptide)
Reactivity:	Human
Mol.Wt.:	54kD(Calculated).
Uniprot:	Q8NB16
RRID:	AB_2847206

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Phospho-MLKL (Thr357/Ser358) Antibody detects endogenous levels of MLKL only when phosphorylated at Thr357/Ser358.

RRID:

AB_2847206
Cite Format: Affinity Biosciences Cat# AF3892, RRID:AB_2847206.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

9130019I15Rik; FLJ34389; hMLKL; Mixed lineage kinase domain like; Mixed lineage kinase domain like protein; Mixed lineage kinase domain like pseudokinase; Mixed lineage kinase domain-like protein; Mlkl; MLKL_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human MLKL around the phosphorylation site of Thr357/Ser358.

Uniprot:

Q8NB16(MLKL_HUMAN) >>Visit HPA database.

Gene(ID):

MLKL(197259)

Sequence:

Q8NB16 MLKL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQYLPPKCMQEIPQEQIKEIKKEQLSGSPWILLRENEVSTLYKGEYHRAPVAIKVFKKLQAGSIAIVRQTFNKEIKTMKKFESPNILRIFGICIDETVTPPQFSIVMEYCELGTLRELLDREKDLTLGKRMVLVLGAARGLYRLHHSEAPELHGKIRSSNFLVTQGYQVKLAGFELRKTQTSMSLGTTREKTDRVKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK

PTMs - Q8NB16 As Substrate

Q8NB16

Site	PTM Type	Enzyme	Source
K40	Ubiquitination		Uniprot
K50	Ubiquitination		Uniprot
S52	Phosphorylation		Uniprot
K57	Ubiquitination		Uniprot
T59	Phosphorylation		Uniprot
K66	Ubiquitination		Uniprot
K78	Ubiquitination		Uniprot
S92	Phosphorylation		Uniprot
S106	Phosphorylation		Uniprot
S125	Phosphorylation		Uniprot
S128	Phosphorylation		Uniprot
K157	Ubiquitination		Uniprot
S161	Phosphorylation		Uniprot
K173	Ubiquitination		Uniprot
K183	Ubiquitination		Uniprot
K198	Ubiquitination		Uniprot
K219	Ubiquitination		Uniprot
K230	Ubiquitination		Uniprot
T246	Phosphorylation		Uniprot
K249	Ubiquitination		Uniprot
T302	Phosphorylation		Uniprot
K331	Ubiquitination		Uniprot
S334	Phosphorylation		Uniprot
K354	Acetylation		Uniprot
K354	Ubiquitination		Uniprot
T357	Phosphorylation	Q9Y572 (RIPK3)	Uniprot
S358	Phosphorylation	Q9Y572 (RIPK3)	Uniprot
T364	Phosphorylation		Uniprot
K372	Ubiquitination		Uniprot
S373	Phosphorylation		Uniprot
T374	Phosphorylation		Uniprot
S393	Phosphorylation		Uniprot
S417	Phosphorylation		Uniprot
S467	Phosphorylation		Uniprot

Research Backgrounds

Q8NB16_MLKL_HUMAN

Function:

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function.

PTMs:

Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.

Subcellular Location:

Cytoplasm. Cell membrane.
Note: Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction.

Subunit Structure:

Homooligomer. Homotrimer; forms homotrimers on necroptosis induction. Interacts with RIPK3; the interaction is direct. Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3. Within this complex, may play a role in the proper targeting of RIPK1/RIPK3 to its downstream effector PGAM5.

Family&Domains:

The protein kinase domain is catalytically inactive but contains an unusual pseudoactive site with an interaction between Lys-230 and Gln-356 residues. Upon phosphorylation by RIPK3, undergoes an active conformation (By similarity).

The coiled coil region 2 is responsible for homotrimerization.

Belongs to the protein kinase superfamily.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Phospho-MLKL (Thr357/Ser358) Antibody - #AF3892