c-Myb Antibody | Affinity Biosciences

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Product:	c-Myb Antibody
Catalog:	AF6136
Description:	Rabbit polyclonal antibody to c-Myb
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	80kDa; 72kD(Calculated).
Uniprot:	P10242
RRID:	AB_2835019

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

c-Myb Antibody detects endogenous levels of total c-Myb.

RRID:

AB_2835019
Cite Format: Affinity Biosciences Cat# AF6136, RRID:AB_2835019.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C myb; C myb protein; Cmyb; MYB; Myb proto oncogene; V myb myeloblastosis viral oncogene homolog;

Immunogens

Immunogen:

Uniprot:

P10242(MYB_HUMAN) >>Visit HPA database.

Gene(ID):

MYB(4602)

Description:

Myb is a transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

Sequence:

P10242 MYB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MARRPRHSIYSSDEDDEDFEMCDHDYDGLLPKSGKRHLGKTRWTREEDEKLKKLVEQNGTDDWKVIANYLPNRTDVQCQHRWQKVLNPELIKGPWTKEEDQRVIELVQKYGPKRWSVIAKHLKGRIGKQCRERWHNHLNPEVKKTSWTEEEDRIIYQAHKRLGNRWAEIAKLLPGRTDNAIKNHWNSTMRRKVEQEGYLQESSKASQPAVATSFQKNSHLMGFAQAPPTAQLPATGQPTVNNDYSYYHISEAQNVSSHVPYPVALHVNIVNVPQPAAAAIQRHYNDEDPEKEKRIKELELLLMSTENELKGQQVLPTQNHTCSYPGWHSTTIADHTRPHGDSAPVSCLGEHHSTPSLPADPGSLPEESASPARCMIVHQGTILDNVKNLLEFAETLQFIDSFLNTSSNHENSDLEMPSLTSTPLIGHKLTVTTPFHRDQTVKTQKENTVFRTPAIKRSILESSPRTPTPFKHALAAQEIKYGPLKMLPQTPSHLVEDLQDVIKQESDESGIVAEFQENGPPLLKKIKQEVESPTDKSGNFFCSHHWEGDSLNTQLFTQTSPVADAPNILTSSVLMAPASEDEDNVLKAFTVPKNRSLASPLQPCSSTWEPASCGKMEEQMTSSSQARKYVNAFSARTLVM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P10242 As Substrate

P10242

Site	PTM Type	Enzyme	Source
S11	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S12	Phosphorylation	P68400 (CSNK2A1)	Uniprot
K53	Ubiquitination		Uniprot
Y69	Phosphorylation		Uniprot
K84	Ubiquitination		Uniprot
K92	Ubiquitination		Uniprot
K97	Ubiquitination		Uniprot
K109	Ubiquitination		Uniprot
K113	Ubiquitination		Uniprot
S116	Phosphorylation	P17612 (PRKACA)	Uniprot
K120	Ubiquitination		Uniprot
C130	S-Nitrosylation		Uniprot
K143	Sumoylation		Uniprot
K143	Ubiquitination		Uniprot
K160	Ubiquitination		Uniprot
K171	Ubiquitination		Uniprot
K182	Ubiquitination		Uniprot
K192	Ubiquitination		Uniprot
K204	Ubiquitination		Uniprot
K291	Ubiquitination		Uniprot
K293	Ubiquitination		Uniprot
K296	Ubiquitination		Uniprot
T420	Phosphorylation		Uniprot
T433	Phosphorylation		Uniprot
K442	Ubiquitination		Uniprot
K445	Acetylation		Uniprot
K445	Ubiquitination		Uniprot
T452	Phosphorylation		Uniprot
R457	Methylation		Uniprot
S463	Phosphorylation		Uniprot
R465	Methylation		Uniprot
T466	Phosphorylation		Uniprot
T468	Phosphorylation		Uniprot
K471	Acetylation		Uniprot
K471	Methylation		Uniprot
K471	Ubiquitination		Uniprot
K480	Acetylation		Uniprot
K480	Sumoylation		Uniprot
K480	Ubiquitination		Uniprot
K485	Acetylation		Uniprot
K485	Ubiquitination		Uniprot
K503	Sumoylation		Uniprot
K527	Sumoylation		Uniprot
S532	Phosphorylation	P27361 (MAPK3)	Uniprot
T534	Phosphorylation		Uniprot
K593	Ubiquitination		Uniprot
S599	Phosphorylation		Uniprot
K615	Ubiquitination		Uniprot
T621	Phosphorylation		Uniprot
S624	Phosphorylation		Uniprot
K628	Ubiquitination		Uniprot

Research Backgrounds

P10242_MYB_HUMAN

Function:

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

PTMs:

Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.

Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.

Phosphorylated by HIPK1. This phosphorylation reduces MYB transcription factor activity but not MYB protein levels.

Subcellular Location:

Nucleus.

Subunit Structure:

Binds MYBBP1A. Interacts with HIPK2, MAF and NLK (By similarity). Binds to HIPK1.

Family&Domains:

Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.

Research Fields

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

References

1). LncRNA AK023391 promotes tumorigenesis and invasion of gastric cancer through activation of the PI3K/Akt signaling pathway. Journal of Experimental & Clinical Cancer Research (PubMed: 29282102) [IF=11.3]

Application: WB Species: human Sample: Gastric cancer cells

Fig. 8 | LncRNA AK023391 was involved in the regulation of the PI3K/Akt signaling pathway.e-f Western blotting validation of the effects of AK023391 knockdown on the expression of PI3K/Akt, NF-κB, p53, and FOXO3a pathways, and their downstream transcription factors c-myb, cyclinB1/G2, and BCL-6 in HGC 27, AGS, and SGC-7901 cells

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For Research Use Only.
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c-Myb Antibody - #AF6136