MSK2 Antibody - #AF6962

Product: MSK2 Antibody
Catalog: AF6962
Description: Rabbit polyclonal antibody to MSK2
Application: ELISA(peptide)
Reactivity: Human, Mouse, Rat
Mol.Wt.: 86kD(Calculated).
Uniprot: O75676
RRID: AB_2847752

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
MSK2 Antibody detects endogenous levels of total MSK2.
RRID:
AB_2847752
Cite Format: Affinity Biosciences Cat# AF6962, RRID:AB_2847752.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

90 kDa ribosomal protein S6 kinase 4; EC 2.7.11.1; KS6A4_HUMAN; Mitogen and stress activated protein kinase 2; Nuclear mitogen and stress activated protein kinase 2; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein kinase B; Ribosomal protein S6 kinase 90kD polypeptide 4; Ribosomal protein S6 kinase 90kDa polypeptide 4; Ribosomal protein S6 kinase alpha 4; Ribosomal protein S6 kinase alpha-4; RPS6KA4; RSKB; S6K-alpha-4;

Immunogens

Immunogen:

A synthesized peptide derived from human MSK2.

Uniprot:

O75676(KS6A4_HUMAN) >>Visit HPA database.

Gene(ID):
RPS6KA4(8986)
Sequence:
MGDEDDDESCAVELRITEANLTGHEEKVSVENFELLKVLGTGAYGKVFLVRKAGGHDAGKLYAMKVLRKAALVQRAKTQEHTRTERSVLELVRQAPFLVTLHYAFQTDAKLHLILDYVSGGEMFTHLYQRQYFKEAEVRVYGGEIVLALEHLHKLGIIYRDLKLENVLLDSEGHIVLTDFGLSKEFLTEEKERTFSFCGTIEYMAPEIIRSKTGHGKAVDWWSLGILLFELLTGASPFTLEGERNTQAEVSRRILKCSPPFPPRIGPVAQDLLQRLLCKDPKKRLGAGPQGAQEVRNHPFFQGLDWVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVARSAMMQDSPFFQQYELDLREPALGQGSFSVCRRCRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLELLRGGELLEHIRKKRHFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQSPGVPMQTPCFTLQYAAPELLAQQGYDESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKLEGLRGSSWLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSATASRRGSPAPANPGRAPVASKGAPRRANGPLPPS

PTMs - O75676 As Substrate

Site PTM Type Enzyme
T22 Phosphorylation
K27 Ubiquitination
S29 Phosphorylation
K37 Ubiquitination
T41 Phosphorylation
Y44 Phosphorylation
K69 Ubiquitination
S87 Phosphorylation
K134 Ubiquitination
Y141 Phosphorylation
S183 Phosphorylation
K184 Ubiquitination
K191 Ubiquitination
T194 Phosphorylation
S196 Phosphorylation Q16539 (MAPK14) , O75676 (RPS6KA4)
K313 Ubiquitination
S324 Phosphorylation P68400 (CSNK2A1)
Y342 Phosphorylation
S343 Phosphorylation Q16539 (MAPK14)
S347 Phosphorylation Q16539 (MAPK14)
Y359 Phosphorylation
S360 Phosphorylation Q16539 (MAPK14)
S365 Phosphorylation
T376 Phosphorylation
K440 Ubiquitination
S502 Phosphorylation
K532 Ubiquitination
T542 Phosphorylation
K548 Ubiquitination
T568 Phosphorylation Q16539 (MAPK14)
S634 Phosphorylation
K648 Ubiquitination
K661 Ubiquitination
K664 Sumoylation
K664 Ubiquitination
S678 Phosphorylation
S681 Phosphorylation
S682 Phosphorylation
T687 Phosphorylation
K720 Ubiquitination
S721 Phosphorylation
K729 Ubiquitination
S737 Phosphorylation
R743 Methylation
S745 Phosphorylation
S772 Phosphorylation

PTMs - O75676 As Enzyme

Substrate Site Source
O75676 (RPS6KA4) S196 Uniprot
O75676-2 (RPS6KA4) T681 Uniprot
O75676-1 (RPS6KA4) T687 Uniprot
P01100 (FOS) S362 Uniprot
P05114 (HMGN1) S7 Uniprot
P16220-1 (CREB1) S133 Uniprot
P18846 (ATF1) S63 Uniprot
Q04206 (RELA) S276 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors.

PTMs:

Ser-343 and Thr-568 phosphorylation is required for kinase activity. Ser-343 and Ser-196 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. Phosphorylated at Ser-343, Thr-568 and Thr-687 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha. Autophosphorylated at Ser-737 and Ser-745 by the N-terminal kinase domain (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA4 associates with and phosphorylates the NF-kappa-B p65 subunit RELA.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

Restrictive clause

 

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