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  • Product Name
    Phospho-p38 MAPK (Thr180/Tyr182) Antibody
  • Catalog No.
    AF4001
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,IP,ELISA
  • Reactivity:
    Human, Mouse, Rat
  • Prediction:
    Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
  • UniProt
  • Mol.Wt.
    43 kDa
  • Concentration
    1mg/ml
  • Browse similar products>>

Product Information

Alternative Names:Expand▼

CSAID Binding Protein 1; CSAID binding protein; CSAID-binding protein; Csaids binding protein; CSBP 1; CSBP 2; CSBP; CSBP1; CSBP2; CSPB1; Cytokine suppressive anti-inflammatory drug-binding protein; EXIP; MAP kinase 14; MAP kinase MXI2; MAP kinase p38 alpha; MAPK 14; MAPK14; MAX interacting protein 2; MAX-interacting protein 2; Mitogen Activated Protein Kinase 14; Mitogen activated protein kinase p38 alpha; Mitogen-activated protein kinase 14; Mitogen-activated protein kinase p38 alpha; MK14_HUMAN; Mxi 2; MXI2; p38 ALPHA; p38; p38 MAP kinase; p38 MAPK; p38 mitogen activated protein kinase; p38ALPHA; p38alpha Exip; PRKM14; PRKM15; RK; SAPK2A;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, IF 1:200, IP, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-p38 MAPK (Thr180/Tyr182) Antibody detects endogenous levels of p38 MAPK only when phosphorylated at Thr180 and Tyr182 .

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Immunogen Information

Immunogen:

A synthesized peptide derived from human p38 MAPK around the phosphorylation site of Thr180/Tyr182 .

Uniprot:



>>Visit The Human Protein Atlas

Gene id:

Molecular Weight:

Observed Mol.Wt.: 43 kDa.
Predicted Mol.Wt.: 42kDa.

Subcellular Location:

Cytoplasm. Nucleus.

Tissue Specificity:

Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.

Description:

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines.

Sequence:
        10         20         30         40         50
MSQERPTFYR QELNKTIWEV PERYQNLSPV GSGAYGSVCA AFDTKTGLRV
60 70 80 90 100
AVKKLSRPFQ SIIHAKRTYR ELRLLKHMKH ENVIGLLDVF TPARSLEEFN
110 120 130 140 150
DVYLVTHLMG ADLNNIVKCQ KLTDDHVQFL IYQILRGLKY IHSADIIHRD
160 170 180 190 200
LKPSNLAVNE DCELKILDFG LARHTDDEMT GYVATRWYRA PEIMLNWMHY
210 220 230 240 250
NQTVDIWSVG CIMAELLTGR TLFPGTDHID QLKLILRLVG TPGAELLKKI
260 270 280 290 300
SSESARNYIQ SLTQMPKMNF ANVFIGANPL AVDLLEKMLV LDSDKRITAA
310 320 330 340 350
QALAHAYFAQ YHDPDDEPVA DPYDQSFESR DLLIDEWKSL TYDEVISFVP
360
PPLDQEEMES

Background

Function:

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. For example, CFLAR is an inhibitor of TNF-induced apoptosis whose proteasome-mediated degradation is regulated by p38 MAPK phosphorylation. In a similar way, MAPK14 phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Another function of MAPK14 is to regulate the endocytosis of membrane receptors by different mechanisms that impinge on the small GTPase RAB5A. In addition, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14-mediated phosphorylation of EGFR itself as well as of RAB5A effectors. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17. Such phosphorylation is required for ADAM17-mediated ectodomain shedding of TGF-alpha family ligands, which results in the activation of EGFR signaling and cell proliferation. Another p38 MAPK substrate is FGFR1. FGFR1 can be translocated from the extracellular space into the cytosol and nucleus of target cells, and regulates processes such as rRNA synthesis and cell growth. FGFR1 translocation requires p38 MAPK activation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, DDIT3, TP53/p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. This phosphorylation enhances the accessibility of the cryptic NF-kappa-B-binding sites marking promoters for increased NF-kappa-B recruitment. Phosphorylates CDC25B and CDC25C which is required for binding to 14-3-3 proteins and leads to initiation of a G2 delay after ultraviolet radiation. Phosphorylates TIAR following DNA damage, releasing TIAR from GADD45A mRNA and preventing mRNA degradation. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation. Protein O-Glc-N-acylation catalyzed by the OGT is regulated by MAPK14, and, although OGT does not seem to be phosphorylated by MAPK14, their interaction increases upon MAPK14 activation induced by glucose deprivation. This interaction may regulate OGT activity by recruiting it to specific targets such as neurofilament H, stimulating its O-Glc-N-acylation. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Also plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis. Phosphorylates S100A9 at 'Thr-113'.

Post-translational Modifications:

Dually phosphorylated on Thr-180 and Tyr-182 by the MAP2Ks MAP2K3/MKK3, MAP2K4/MKK4 and MAP2K6/MKK6 in response to inflammatory citokines, environmental stress or growth factors, which activates the enzyme. Dual phosphorylation can also be mediated by TAB1-mediated autophosphorylation. TCR engagement in T-cells also leads to Tyr-323 phosphorylation by ZAP70. Dephosphorylated and inactivated by DUPS1, DUSP10 and DUSP16. PPM1D also mediates dephosphorylation and inactivation of MAPK14 (PubMed:21283629).Acetylated at Lys-53 and Lys-152 by KAT2B and EP300. Acetylation at Lys-53 increases the affinity for ATP and enhances kinase activity. Lys-53 and Lys-152 are deacetylated by HDAC3.Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway.

Subcellular Location:

Nucleus;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK (PubMed:21224381). Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR (By similarity). This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation (By similarity). Interacts with SPAG9 and GADD45A (By similarity). Interacts with CDC25B, CDC25C, DUSP1, DUSP10, DUSP16, NP60, SUPT20H and TAB1. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B. Interacts with PPM1D. Interacts with CDK5RAP3; recruits PPM1D to MAPK14 and may regulate its dephosphorylation (PubMed:21283629).

Similarity:

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Research Fields

Research Fields:

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.(View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence.(View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.(View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.(View pathway)
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.(View pathway)
· Organismal Systems > Immune system > Toll-like receptor signaling pathway.(View pathway)
· Organismal Systems > Immune system > IL-17 signaling pathway.(View pathway)
· Organismal Systems > Immune system > Platelet activation.(View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway.(View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.(View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration.(View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
· Organismal Systems > Endocrine system > Relaxin signaling pathway.
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.(View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation.(View pathway)
· Organismal Systems > Endocrine system > Prolactin signaling pathway.(View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway.(View pathway)
· Organismal Systems > Nervous system > Dopaminergic synapse.
· Organismal Systems > Development > Osteoclast differentiation.(View pathway)
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.(View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway.(View pathway)
· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.(View pathway)

Western blot analysis of extracts from Colo205, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody. The lane on the left was treated with blocking peptide.
AF4001 at 1/200 staining human kidney tissue sections by IHC-P.
AF4001 staining 293T treated with UV 30min by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ELISA analysis of AF4001 showing specificity to Phospho-p38 MAPK (Thr180/Tyr182) peptide. Peptides concentration: 1ug/ml.
P-peptide: phospho-peptide; N-peptide: non-phospho-peptide.

Reference Citations:

1). Geng J et al. Trimethylamine N-oxide promotes atherosclerosis via CD36-dependent MAPK/JNK pathway. Biomed Pharmacother 2018 Jan;97:941-947 (PubMed: 29136772) [IF=3.743]

2). Ma L et al. I4, a synthetic anti-diabetes agent, attenuates atherosclerosis through its lipid-lowering, anti-inflammatory and anti-apoptosis properties. Mol Cell Endocrinol 2017 Jan 15;440:80-92 (PubMed: 27725191) [IF=3.693]

Application: WB    Species:mouse;    Sample:Not available

Fig.3 I4 suppressed in vivo expression of MAPK/NF-κB signaling pathway and decreased inflammatory marker production. Mouse serum inflammatory marker levels: (A) IL-6, (B)IL-1β, (C) IL-10 and (D) TNF-α. (n = 8~10/group, ** p<0.01 and * p<0.05 vs model group). (E)The levels of NF-κB p65, ERK1/2 and p38 MAPK phosphorylation in mouse aorta of different treatment groups.


3). Tan L et al. Dihydroberberine, a hydrogenated derivative of berberine firstly identified in Phellodendri Chinese Cortex, exerts anti-inflammatory effect via dual modulation of NF-κB and MAPK signaling pathways. Int Immunopharmacol 2019 Aug 8;75:105802 (PubMed: 31401380) [IF=3.361]

4). Xing J et al. Epicatechin alleviates inflammation in lipopolysaccharide-induced acute lung injury in mice by inhibiting the p38 MAPK signaling pathway. Int Immunopharmacol 2018 Nov 16;66:146-153 (PubMed: 30453148) [IF=3.361]

5). Zhao J et al. Dehydroepiandrosterone alleviates E. Coli O157:H7-induced inflammation by preventing the activation of p38 MAPK and NF-κB pathways in mice peritoneal macrophages. Mol Immunol 2019 Jul 24;114:114-122 (PubMed: 31351412) [IF=3.064]

6). Lu M et al. CTGF Triggers Rat Astrocyte Activation and Astrocyte-Mediated Inflammatory Response in Culture Conditions. Inflammation 2019 Jun 10 (PubMed: 31183597)

7). Cao J et al. SUMO2 modification of Aurora B and its impact on follicular development and atresia in the mouse ovary. Int J Mol Med 2018 Jun;41(6):3115-3126 (PubMed: 29512695)

8). Liu Y et al. Fucoxanthin Activates Apoptosis via Inhibition of PI3K/Akt/mTOR Pathway and Suppresses Invasion and Migration by Restriction of p38-MMP-2/9 Pathway in Human Glioblastoma Cells. Neurochem Res 2016 Oct;41(10):2728-2751 (PubMed: 27394418)

9). Huang L et al. Neuroprotective Effect of Curcumin Against Cerebral Ischemia-Reperfusion Via Mediating Autophagy and Inflammation. J Mol Neurosci 2018 Jan;64(1):129-139 (PubMed: 29243061)

10). Xing Y et al. Electroacupuncture Alleviated Neuronal Apoptosis Following Ischemic Stroke in Rats via Midkine and ERK/JNK/p38 Signaling Pathway. J Mol Neurosci 2018 Jul 31 (PubMed: 30062439)

11). Wang X et al. Oxymatrine inhibits the migration of human colorectal carcinoma RKO cells via inhibition of PAI-1 and the TGF-β1/Smad signaling pathway. Oncol Rep 2017 Feb;37(2):747-753 (PubMed: 27959430)

Application: WB    Species:human;    Sample:Not available

Figure 5. Effect of oxymatrine (OM) on the expression of TGF-β1, pSmad2, PP38, Smad4 and PAI-1 proteins by western blotting. Western blotting demonstrated that the expression levels of TGF-β1 and PAI-1 protein were markedly increased in the UR group as compared to the FHC group. The expression of TGF-β1 and PAI-1 proteins was significantly decreased in the OR group as compared to the UR group. Western blotting also demonstrated that the expression level of Smad4 and the phosphorylation of Smad2 and P38 were markedly increased in the UR group as compared to the FHC group. The expression level of Smad4 and the phosphorylation of Smad2 and P38 were significantly decreased in the OR group as compared to the UR group. * P<0.05, **P<0.01.


12). Yang Z et al. The mechanically activated p38/MMP-2 signaling pathway promotes bone marrow mesenchymal stem cell migration in rats. Arch Oral Biol 2017 Apr;76:55-60 (PubMed: 28126687)

Application: WB    Species:rat;    Sample:Not available

Fig. 3. Strain-induced MMP-2 expression in BMMSCs was mediated by p38 activity. BMMSCs in the experimental group were subjected to mechanical strain (static, 6%) for 2 h. No strain was applied to BMMSCs in the control group. Both p-p38 and MMP-2 were upregulated in BMMSCs by the strain. The effect was abolished by inhibition of p38 phosphorylation. Data represent the mean  SEM. *P < 0.05 and **P < 0.01 by Student’s t test.


13). Zhou Y et al. Ligustilide attenuates nitric oxide-induced apoptosis in rat chondrocytes and cartilage degradation via inhibiting JNK and p38 MAPK pathways. J Cell Mol Med 2019 Feb 15 (PubMed: 30770640)

14). Ying-yuLu et al. Phloridzin alleviate colitis in mice by protecting the intestinal brush border and improving the expression of sodium glycogen transporter 1. J FUNCT FOODS 2018;45:348-354

15). et al. Wnt/β‑catenin signaling pathway regulates asthma airway remodeling by influencing the expression of c‑Myc and cyclin D1 via the p38 MAPK‑dependent pathway.

16). LingmanMa et al. 1I 4, a synthetic anti-diabetes agent, attenuates atherosclerosis through its lipid-lowering, anti-inflammatory and anti-apoptosis properties. Molecular and Cellular Endocrinology 2017;440:80-92

17). Huikun Xu et al. Chemerin promotes the viability and migration of human placental microvascular endothelial cells and activates MAPK/AKT signaling . Int J Clin Exp Med 2018;11(2):721-727

18). et al. Transforming Growth Factor-β3 Chitosan Sponge (TGF-β3/CS) Facilitates Osteogenic Differentiation of Human Periodontal Ligament Stem Cells.

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Catalog Number :

AF4001-BP
(Blocking peptide available as AF4001-BP)

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Rabbit
100%
Sheep
100%
Dog
100%
Horse
100%
Bovine
100%
Xenopus
91%
Zebrafish
0%
Chicken
0%
High similarity Medium similarity Low similarity No similarity
Q16539 as Substrate
Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
T7 Phosphorylation
K15 Ubiquitination
T16 Phosphorylation
S28 Phosphorylation
S32 Phosphorylation
K45 Ubiquitination
K53 Acetylation
K54 Ubiquitination
S56 Phosphorylation
S61 Phosphorylation
K66 Ubiquitination
K79 Sumoylation
K79 Ubiquitination
K118 Ubiquitination
K121 Ubiquitination
T123 Phosphorylation P25098 (GRK2)
K139 Ubiquitination
S143 Phosphorylation
K152 Acetylation
K152 Ubiquitination
K165 Ubiquitination
T175 Phosphorylation
T180 Phosphorylation P46734 (MAP2K3) , P52564 (MAP2K6) , P07949 (RET) , P45985 (MAP2K4) , Q99683 (MAP3K5) , O95382 (MAP3K6) , Q16539 (MAPK14)
Y182 Phosphorylation P52564 (MAP2K6) , O95382 (MAP3K6) , P45985 (MAP2K4) , Q16539 (MAPK14) , O14733-2 (MAP2K7) , Q99683 (MAP3K5) , P46734 (MAP2K3) , P07949 (RET)
T185 Phosphorylation
K233 Ubiquitination
T241 Phosphorylation
K248 Ubiquitination
K249 Ubiquitination
R256 Methylation
T263 Phosphorylation
K295 Ubiquitination
Y323 Phosphorylation P06241 (FYN) , P06239 (LCK) , P43403 (ZAP70)
S326 Phosphorylation
K338 Sumoylation
S339 Phosphorylation
Q16539 as PTM Enzyme
Substrate Site Source
F1D8S2 (NR2A1) S167 Uniprot
O00418 (EEF2K) S396 Uniprot
O15198 (SMAD9) T136 Uniprot
O43255 (SIAH2) S28 Uniprot
O43257 (ZNHIT1) T103 Uniprot
O43521 (BCL2L11) S69 Uniprot
O43524 (FOXO3) S7 Uniprot
O43524 (FOXO3) S12 Uniprot
O43524 (FOXO3) S294 Uniprot
O43524 (FOXO3) S344 Uniprot
O43524 (FOXO3) S425 Uniprot
O60381 (HBP1) S402 Uniprot
O75030 (MITF) S414 Uniprot
O75582 (RPS6KA5) S360 Uniprot
O75582-1 (RPS6KA5) S376 Uniprot
O75582 (RPS6KA5) T581 Uniprot
O75582 (RPS6KA5) T700 Uniprot
O75676 (RPS6KA4) S196 Uniprot
O75676-2 (RPS6KA4) S343 Uniprot
O75676-2 (RPS6KA4) S347 Uniprot
O75676 (RPS6KA4) S360 Uniprot
O75676-2 (RPS6KA4) T562 Uniprot
O75676 (RPS6KA4) T568 Uniprot
O95644 (NFATC1) S172 Uniprot
P00533 (EGFR) T693 Uniprot
P00533 (EGFR) S1039 Uniprot
P00533 (EGFR) T1041 Uniprot
P02511 (CRYAB) S59 Uniprot
P03372 (ESR1) S118 Uniprot
P03372 (ESR1) S294 Uniprot
P03372-1 (ESR1) T311 Uniprot
P04150 (NR3C1) S203 Uniprot
P04150 (NR3C1) S211 Uniprot
P04150 (NR3C1) S226 Uniprot
P04637 (TP53) S15 Uniprot
P04637 (TP53) S33 Uniprot
P04637 (TP53) S46 Uniprot
P04637-1 (TP53) S392 Uniprot
P04792 (HSPB1) S176 Uniprot
P05198 (EIF2S1) S52 Uniprot
P05787-1 (KRT8) S74 Uniprot
P06400 (RB1) S567 Uniprot
P06702 (S100A9) T113 Uniprot
P10275 (AR) S651 Uniprot
P10415 (BCL2) T56 Uniprot
P10415 (BCL2) S87 Uniprot
P10636-8 (MAPT) T50 Uniprot
P10636-8 (MAPT) T69 Uniprot
P10636-8 (MAPT) T153 Uniprot
P10636-8 (MAPT) S202 Uniprot
P10636-8 (MAPT) T205 Uniprot
P10636-8 (MAPT) S235 Uniprot
P10636-8 (MAPT) S404 Uniprot
P10636-8 (MAPT) S422 Uniprot
P11362 (FGFR1) S777 Uniprot
P13726 (F3) S285 Uniprot
P13726 (F3) S290 Uniprot
P14598 (NCF1) S345 Uniprot
P14598 (NCF1) S348 Uniprot
P15336 (ATF2) T69 Uniprot
P15336 (ATF2) T71 Uniprot
P15336-1 (ATF2) S90 Uniprot
P15923 (TCF3) S139 Uniprot
P16220 (CREB1) S133 Uniprot
P16949 (STMN1) S25 Uniprot
P17181 (IFNAR1) S532 Uniprot
P17275 (JUNB) S79 Uniprot
P17275 (JUNB) T102 Uniprot
P17275 (JUNB) T104 Uniprot
P17302 (GJA1) S279 Uniprot
P17302 (GJA1) S282 Uniprot
P17844 (DDX5) T446 Uniprot
P17844 (DDX5) T564 Uniprot
P17861 (XBP1) S68 Uniprot
P18850 (ATF6) T166 Uniprot
P19419 (ELK1) S383 Uniprot
P19419 (ELK1) S389 Uniprot
P19525 (EIF2AK2) T451 Uniprot
P19634 (SLC9A1) T718 Uniprot
P19634 (SLC9A1) S723 Uniprot
P19634 (SLC9A1) S726 Uniprot
P19634-1 (SLC9A1) S729 Uniprot
P21397 (MAOA) S209 Uniprot
P21462 (FPR1) S342 Uniprot
P22415 (USF1) T153 Uniprot
P28324 (ELK4) S381 Uniprot
P28324 (ELK4) S387 Uniprot
P28698 (MZF1) S256 Uniprot
P28698 (MZF1) S274 Uniprot
P28698 (MZF1) S294 Uniprot
P29353 (SHC1) S36 Uniprot
P29353 (SHC1) S54 Uniprot
P29353 (SHC1) T56 Uniprot
P29353 (SHC1) T386 Uniprot
P30279 (CCND2) T280 Uniprot
P30305 (CDC25B) S323 Uniprot
P30305 (CDC25B) S375 Uniprot
P30307 (CDC25C) S216 Uniprot
P31645 (SLC6A4) T616 Uniprot
P35236 (PTPN7) T66 Uniprot
P35236 (PTPN7) S93 Uniprot
P35236-2 (PTPN7) T105 Uniprot
P35236-2 (PTPN7) S132 Uniprot
P35638-1 (DDIT3) S79 Uniprot
P35638-1 (DDIT3) S82 Uniprot
P36956-3 (SREBF1) S39 Uniprot
P36956 (SREBF1) S63 Uniprot
P36956-3 (SREBF1) T402 Uniprot
P36956 (SREBF1) T426 Uniprot
P38936 (CDKN1A) T57 Uniprot
P38936 (CDKN1A) S130 Uniprot
P40763 (STAT3) S727 Uniprot
P41212 (ETV6) S22 Uniprot
P41212 (ETV6) S257 Uniprot
P41235-5 (HNF4A) S145 Uniprot
P41235 (HNF4A) S167 Uniprot
P41970 (ELK3) S357 Uniprot
P41970 (ELK3) S363 Uniprot
P42224 (STAT1) S727 Uniprot
P42566 (EPS15) S796 Uniprot
P42574 (CASP3) S150 Uniprot
P42677 (RPS27) S27 Uniprot
P47712 (PLA2G4A) S505 Uniprot
P49023 (PXN) S85 Uniprot
P49137-2 (MAPKAPK2) T25 Uniprot
P49137-2 (MAPKAPK2) T206 Uniprot
P49137-2 (MAPKAPK2) T222 Uniprot
P49137 (MAPKAPK2) S272 Uniprot
P49137-1 (MAPKAPK2) T317 Uniprot
P49137-1 (MAPKAPK2) T334 Uniprot
P49841 (GSK3B) S389 Uniprot
P49841 (GSK3B) T390 Uniprot
P49918 (CDKN1C) S146 Uniprot
P52945 (PDX1) S61 Uniprot
P52945 (PDX1) S66 Uniprot
P53667 (LIMK1) S310 Uniprot
P56178 (DLX5) S34 Uniprot
P56178 (DLX5) S217 Uniprot
P61244-2 (MAX) S40 Uniprot
P61244-6 (MAX) S49 Uniprot
P61244-2 (MAX) S135 Uniprot
P61244-1 (MAX) S144 Uniprot
P68431 (HIST1H3J) S11 Uniprot
P68431 (HIST1H3J) S29 Uniprot
P78356 (PIP4K2B) S326 Uniprot
P78536 (ADAM17) T735 Uniprot
P78543 (BTG2) S149 Uniprot
P84022 (SMAD3) S204 Uniprot
P84022 (SMAD3) S208 Uniprot
P84022-1 (SMAD3) S213 Uniprot
Q01844 (EWSR1) T79 Uniprot
Q02078 (MEF2A) S98 Uniprot
Q02078 (MEF2A) T108 Uniprot
Q02078 (MEF2A) S192 Uniprot
Q02078 (MEF2A) S223 Uniprot
Q02078-5 (MEF2A) T304 Uniprot
Q02078-5 (MEF2A) T311 Uniprot
Q02078 (MEF2A) T312 Uniprot
Q02078 (MEF2A) T319 Uniprot
Q02078 (MEF2A) S355 Uniprot
Q02078 (MEF2A) S408 Uniprot
Q02078-5 (MEF2A) S445 Uniprot
Q02078 (MEF2A) S453 Uniprot
Q02078 (MEF2A) S479 Uniprot
Q02078 (MEF2A) S494 Uniprot
Q02156 (PRKCE) S350 Uniprot
Q06330 (RBPJ) T339 Uniprot
Q06413 (MEF2C) T293 Uniprot
Q06413 (MEF2C) T300 Uniprot
Q06413 (MEF2C) S387 Uniprot
Q07666 (KHDRBS1) S58 Uniprot
Q07666 (KHDRBS1) T84 Uniprot
Q07817 (BCL2L1) S62 Uniprot
Q12778 (FOXO1) S416 Uniprot
Q12778 (FOXO1) S432 Uniprot
Q12778 (FOXO1) S470 Uniprot
Q12778 (FOXO1) T478 Uniprot
Q12778 (FOXO1) T560 Uniprot
Q13541 (EIF4EBP1) T37 Uniprot
Q13541 (EIF4EBP1) T46 Uniprot
Q13541 (EIF4EBP1) S65 Uniprot
Q13541 (EIF4EBP1) T70 Uniprot
Q14721 (KCNB1) S805 Uniprot
Q14765 (STAT4) S721 Uniprot
Q14790 (CASP8) S347 Uniprot
Q14934 (NFATC4) S168 Uniprot
Q14934 (NFATC4) S170 Uniprot
Q15046 (KARS) T52 Uniprot
Q15075 (EEA1) T1392 Uniprot
Q15596 (NCOA2) S736 Uniprot
Q15672 (TWIST1) S68 Uniprot
Q15717 (ELAVL1) T118 Uniprot
Q15750 (TAB1) S423 Uniprot
Q15750 (TAB1) T431 Uniprot
Q15750 (TAB1) S438 Uniprot
Q15750 (TAB1) S452 Uniprot
Q15750 (TAB1) S453 Uniprot
Q15750 (TAB1) S456 Uniprot
Q15750 (TAB1) S457 Uniprot
Q15910 (EZH2) T367 Uniprot
Q16539 (MAPK14) T180 Uniprot
Q16539 (MAPK14) Y182 Uniprot
Q86UR1 (NOXA1) S239 Uniprot
Q86UR1 (NOXA1) S282 Uniprot
Q8IW41-1 (MAPKAPK5) T182 Uniprot
Q8NHW3 (MAFA) S14 Uniprot
Q8NHW3 (MAFA) T57 Uniprot
Q8NHW3 (MAFA) T134 Uniprot
Q8TDD2 (SP7) S76 Uniprot
Q8TDD2 (SP7) S80 Uniprot
Q8WYK2-1 (JDP2) T148 Uniprot
Q92945 (KHSRP) T692 Uniprot
Q92993-2 (KAT5) T106 Uniprot
Q92993 (KAT5) T158 Uniprot
Q99460 (PSMD1) T273 Uniprot
Q99626 (CDX2) S283 Uniprot
Q99626 (CDX2) S287 Uniprot
Q99626 (CDX2) S291 Uniprot
Q99626 (CDX2) S295 Uniprot
Q9BR76 (CORO1B) S2 Uniprot
Q9BUB5-2 (MKNK1) T209 Uniprot
Q9BUB5-2 (MKNK1) T214 Uniprot
Q9BUB5 (MKNK1) T250 Uniprot
Q9BUB5 (MKNK1) T255 Uniprot
Q9H1K0 (RBSN) S215 Uniprot
Q9NQU5 (PAK6) S165 Uniprot
Q9NRR4 (DROSHA) S221 Uniprot
Q9NRR4 (DROSHA) S255 Uniprot
Q9NRR4 (DROSHA) T274 Uniprot
Q9NRR4 (DROSHA) S300 Uniprot
Q9NRR4 (DROSHA) S355 Uniprot
Q9UBK2-1 (PPARGC1A) T263 Uniprot
Q9UBK2 (PPARGC1A) S266 Uniprot
Q9UBK2 (PPARGC1A) T299 Uniprot
Q9UIG0 (BAZ1B) S158 Uniprot
Q9UL54-2 (TAOK2) S1031 Uniprot
Q9UQD0 (SCN8A) S553 Uniprot
Q9Y5Y9 (SCN10A) S552 Uniprot
Q9Y698 (CACNG2) T321 Uniprot
Q9Y6Q9 (NCOA3) T24 Uniprot
Q9Y6Q9 (NCOA3) S505 Uniprot
Q9Y6Q9-1 (NCOA3) S543 Uniprot
Q9Y6Q9 (NCOA3) S860 Uniprot
Q9Y6Q9-5 (NCOA3) S867 Uniprot
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.