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  • Product Name
    ABCG2 Antibody
  • Catalog No.
    AF4041
  • RRID
    AB_2835345
  • Source
    Rabbit
  • Application
    WB,ELISA
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Rabbit(88%)
  • UniProt
  • Mol.Wt
    65-80kD;
    72kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

ABC transporter; ABC15; ABCG 2; ABCG2; ABCG2_HUMAN; ABCP; ATP binding cassette sub family G (WHITE) member 2; ATP binding cassette transporter G2; ATP-binding cassette sub-family G member 2; BCRP; BCRP1; BMDP; Breast cancer resistance protein; CD338; CDw338; CDw338 antigen; EST157481; GOUT1; MGC102821; Mitoxantrone resistance associated protein; Mitoxantrone resistance-associated protein; MRX; Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2; MXR; MXR1; Placenta specific ATP binding cassette transporter; Placenta specific MDR protein; Placenta-specific ATP-binding cassette transporter; UAQTL1;

Applications:

WB 1:500-1:1000, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Rabbit(88%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

ABCG2 Antibody detects endogenous levels of total ABCG2.

RRID:

AB_2835345
Please cite this product as: Affinity Biosciences Cat# AF4041, RRID:AB_2835345.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human ABCG2(CD338), corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

ABCG2

Molecular Weight:

Observed Mol.Wt.: 65-80kD.
Predicted Mol.Wt.: 72kDa(Calculated)..

Subcellular Location:

Cell membrane.

Tissue Specificity:

Q9UNQ0 ABCG2_HUMAN:
Highly expressed in placenta (PubMed:9850061). Low expression in small intestine, liver and colon (PubMed:9861027). Expressed in brain (at protein level) (PubMed:12958161).

Description:

Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.

Sequence:
MSSSNVEVFIPVSQGNTNGFPATASNDLKAFTEGAVLSFHNICYRVKLKSGFLPCRKPVEKEILSNINGIMKPGLNAILGPTGGGKSSLLDVLAARKDPSGLSGDVLINGAPRPANFKCNSGYVVQDDVVMGTLTVRENLQFSAALRLATTMTNHEKNERINRVIQELGLDKVADSKVGTQFIRGVSGGERKRTSIGMELITDPSILFLDEPTTGLDSSTANAVLLLLKRMSKQGRTIIFSIHQPRYSIFKLFDSLTLLASGRLMFHGPAQEALGYFESAGYHCEAYNNPADFFLDIINGDSTAVALNREEDFKATEIIEPSKQDKPLIEKLAEIYVNSSFYKETKAELHQLSGGEKKKKITVFKEISYTTSFCHQLRWVSKRSFKNLLGNPQASIAQIIVTVVLGLVIGAIYFGLKNDSTGIQNRAGVLFFLTTNQCFSSVSAVELFVVEKKLFIHEYISGYYRVSSYFLGKLLSDLLPMRMLPSIIFTCIVYFMLGLKPKADAFFVMMFTLMMVAYSASSMALAIAAGQSVVSVATLLMTICFVFMMIFSGLLVNLTTIASWLSWLQYFSIPRYGFTALQHNEFLGQNFCPGLNATGNNPCNYATCTGEEYLVKQGIDLSPWGLWKNHVALACMIVIFLTIAYLKLLFLKKYS

Research Background

Function:

Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes a wide variety of physiological compounds, dietary toxins and xenobiotics from cells. Involved in porphyrin homeostasis, mediating the export of protoporphyrin IX (PPIX) from both mitochondria to cytosol and cytosol to extracellular space, it also functions in the cellular export of heme. Also mediates the efflux of sphingosine-1-P from cells. Acts as a urate exporter functioning in both renal and extrarenal urate excretion. In kidney, it also functions as a physiological exporter of the uremic toxin indoxyl sulfate (By similarity). Also involved in the excretion of steroids like estrone 3-sulfate/E1S, 3beta-sulfooxy-androst-5-en-17-one/DHEAS, and other sulfate conjugates. Mediates the secretion of the riboflavin and biotin vitamins into milk (By similarity). Extrudes pheophorbide a, a phototoxic porphyrin catabolite of chlorophyll, reducing its bioavailability (By similarity). Plays an important role in the exclusion of xenobiotics from the brain (Probable). It confers to cells a resistance to multiple drugs and other xenobiotics including mitoxantrone, pheophorbide, camptothecin, methotrexate, azidothymidine, and the anthracyclines daunorubicin and doxorubicin, through the control of their efflux. In placenta, it limits the penetration of drugs from the maternal plasma into the fetus (By similarity). May play a role in early stem cell self-renewal by blocking differentiation (By similarity).

Post-translational Modifications:

N-glycosylated. Glycosylation-deficient ABCG2 is normally expressed and functional.

Phosphorylated. Phosphorylation at Thr-362 by PIM1 is induced by drugs like mitoxantrone and is associated with cells increased drug resistance. It regulates the localization to the plasma membrane, the homooligomerization and therefore, the activity of the transporter.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Apical cell membrane>Multi-pass membrane protein. Mitochondrion membrane>Multi-pass membrane protein.
Note: Enriched in membrane lipid rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Highly expressed in placenta. Low expression in small intestine, liver and colon. Expressed in brain (at protein level).

Subunit Structure:

Homodimer; disulfide-linked. The minimal functional unit is a homodimer, but the major oligomeric form in plasma membrane is a homotetramer with possibility of higher order oligomerization up to homododecamers.

Similarity:

The extracellular loop 3 (ECL3) is involved in binding porphyrins and transfer them to other carriers, probably albumin.

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

Research Fields

Research Fields:

· Environmental Information Processing > Membrane transport > ABC transporters.
· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

Reference Citations:

1). Xun T et al. Advanced oxidation protein products upregulate efflux transporter expression and activity through activation of the Nrf-2-mediated signaling pathway in vitro and in vivo. Eur J Pharm Sci 2020 Apr 18:105342 (PubMed: 32315774) [IF=3.616]

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Catalog Number :

AF4041-BP
(Blocking peptide available as AF4041-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Rabbit
88%
Sheep
75%
Bovine
75%
Horse
75%
Pig
63%
Chicken
0%
Dog
0%
Xenopus
0%
Zebrafish
0%
High similarity Medium similarity Low similarity No similarity
Q9UNQ0 as Substrate
Site PTM Type Enzyme
S50 Phosphorylation
S100 Phosphorylation
S103 Phosphorylation
S143 Phosphorylation
K172 Ubiquitination
S187 Phosphorylation
T194 Phosphorylation
S195 Phosphorylation
K323 Ubiquitination
S353 Phosphorylation
K357 Ubiquitination
T362 Phosphorylation P11309 (PIM1)
Y464 Phosphorylation
N596 N-Glycosylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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