CLK1 Antibody - #DF3189

(2)  
Product: CLK1 Antibody
Catalog: DF3189
Description: Rabbit polyclonal antibody to CLK1
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Rabbit, Dog
Mol.Wt.: 57 KD; 57kD(Calculated).
Uniprot: P49759
RRID: AB_2835412

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Rabbit(88%), Dog(89%)
Clonality:
Polyclonal
Specificity:
CLK1 Antibody detects endogenous levels of total CLK1.
RRID:
AB_2835412
Cite Format: Affinity Biosciences Cat# DF3189, RRID:AB_2835412.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CDC like kinase 1; CDC-like kinase 1; CDC28/CDC2 like kinase; CLK; CLK/STY; Clk1; CLK1_HUMAN; Dual specificity protein kinase CLK1; Protein tyrosine kinase STY; STY;

Immunogens

Immunogen:
Uniprot:

P49759(CLK1_HUMAN) >>Visit HPA database.

Gene(ID):
CLK1(1195)
Expression:
P49759 CLK1_HUMAN:

Endothelial cells.

Sequence:
MRHSKRTYCPDWDDKDWDYGKWRSSSSHKRRKRSHSSAQENKRCKYNHSKMCDSHYLESRSINEKDYHSRRYIDEYRNDYTQGCEPGHRQRDHESRYQNHSSKSSGRSGRSSYKSKHRIHHSTSHRRSHGKSHRRKRTRSVEDDEEGHLICQSGDVLSARYEIVDTLGEGAFGKVVECIDHKAGGRHVAVKIVKNVDRYCEAARSEIQVLEHLNTTDPNSTFRCVQMLEWFEHHGHICIVFELLGLSTYDFIKENGFLPFRLDHIRKMAYQICKSVNFLHSNKLTHTDLKPENILFVQSDYTEAYNPKIKRDERTLINPDIKVVDFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFPTHDSKEHLAMMERILGPLPKHMIQKTRKRKYFHHDRLDWDEHSSAGRYVSRRCKPLKEFMLSQDVEHERLFDLIQKMLEYDPAKRITLREALKHPFFDLLKKSI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
89
Rabbit
88
Pig
78
Sheep
78
Bovine
38
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49759 As Substrate

Site PTM Type Enzyme
K15 Ubiquitination
K21 Sumoylation
K21 Ubiquitination
S24 Phosphorylation
S34 Phosphorylation
S36 Phosphorylation
S37 Phosphorylation
K50 Sumoylation
K50 Ubiquitination
S54 Phosphorylation
Y56 Phosphorylation
S59 Phosphorylation
S61 Phosphorylation
K65 Ubiquitination
Y76 Phosphorylation
Y80 Phosphorylation
S101 Phosphorylation
S102 Phosphorylation
S104 Phosphorylation
S122 Phosphorylation
T123 Phosphorylation
T138 Phosphorylation
S140 Phosphorylation
S158 Phosphorylation
K174 Ubiquitination
K182 Ubiquitination
K191 Sumoylation
K191 Ubiquitination
T285 Phosphorylation
K290 Ubiquitination
S299 Phosphorylation
Y305 Phosphorylation
K308 Ubiquitination
K322 Sumoylation
S328 Phosphorylation
T330 Phosphorylation
S337 Phosphorylation
T338 Phosphorylation
S341 Phosphorylation
T342 Phosphorylation
Y345 Phosphorylation
K400 Ubiquitination
K464 Ubiquitination
K481 Ubiquitination

PTMs - P49759 As Enzyme

Substrate Site Source
P00519 (ABL1) T735 Uniprot
P18031 (PTPN1) S50 Uniprot
P18031 (PTPN1) S242 Uniprot
P18031 (PTPN1) S243 Uniprot
Q96I25 (RBM17) S48 Uniprot
Q96I25 (RBM17) S62 Uniprot
Q96I25 (RBM17) S202 Uniprot
Q96I25 (RBM17) S204 Uniprot
Q96I25 (RBM17) S222 Uniprot
Q96I25 (RBM17) S266 Uniprot
Q96I25 (RBM17) S288 Uniprot
Q96I25 (RBM17) S291 Uniprot

Research Backgrounds

Function:

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates: SRSF1, SRSF3 and PTPN1. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells and adenovirus E1A pre-mRNA.

PTMs:

Autophosphorylates on all three types of residues.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Endothelial cells.

Subunit Structure:

Interacts with PPIG and UBL5.

Family&Domains:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

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