TTC23 Antibody | Affinity Biosciences

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Western blot analysis of extracts from RAW264.

Product:	TTC23 Antibody
Catalog:	DF3320
Description:	Rabbit polyclonal antibody to TTC23
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	50 KD; 50kD(Calculated).
Uniprot:	Q5W5X9
RRID:	AB_2835687

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

TTC23 Antibody detects endogenous levels of total TTC23.

RRID:

AB_2835687
Cite Format: Affinity Biosciences Cat# DF3320, RRID:AB_2835687.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cervical cancer proto oncogene 8 protein; Cervical cancer proto-oncogene 8 protein; HCC 8; HCC-8; OTTHUMP00000190851; Protooncogene 8; Tetratricopeptide repeat domain 23; Tetratricopeptide repeat protein 23; TPR repeat protein 23; Ttc23; TTC23_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q5W5X9(TTC23_HUMAN) >>Visit HPA database.

Gene(ID):

TTC23(64927)

Sequence:

Q5W5X9 TTC23_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQESQETHISNHLDEVVAAVSITHRKKFQNKLLQTALFQPPREKLHLCEEKAKSYSNSHEYKQAVHELVRCVALTRICYGDSHWKLAEAHVNLAQGYLQLKGLSLQAKQHAEKARQILANSIVPPYSENTDVFKFSIELFHTMGRALLSLQKFKEAAENLTKAERLSKELLQCGRIIKEEWIEIEARIRLSFAQVYQGQKKSKEALSHYQAALEYVEISKGETSRECVPILRELAGVEQALGLHDVSINHFLQAHLIILSRSPSQVEAADSAHIVAHAAVASGRHEHHDVAEQYFQESMAHLKDSEGMGRTKFLSIQDEFCHFLQMTGQKERATSILRESLEAKVEAFGDFSPEVAETYRLLGGADLAQGNHSGARKKLKKCLQIQTLLYGPQDKRTLATQQAMGMLSTAPKVASKPRQASKAKVAFCTSIPQDTLLGKARPGTTAD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5W5X9 As Substrate

Q5W5X9

Site	PTM Type	Source
T161	Phosphorylation	Uniprot
S167	Phosphorylation	Uniprot
S415	Phosphorylation	Uniprot

Research Backgrounds

Q5W5X9_TTC23_HUMAN

Function:

Participates positively in the ciliary Hedgehog (Hh) signaling.

Subcellular Location:

Cell projection>Cilium.
Note: Colocalizes with EVC and IQCE at the EvC zone of primary cilia.

Subunit Structure:

Found Associated with the EvC complex composed of EFCAB7, IQCE, EVC2 and EVC.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

TTC23 Antibody - #DF3320