RPS6KC1 Antibody - #DF3408

(3)  
Product: RPS6KC1 Antibody
Catalog: DF3408
Description: Rabbit polyclonal antibody to RPS6KC1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 117 KD; 119kD(Calculated).
Uniprot: Q96S38
RRID: AB_2835790

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
RPS6KC1 Antibody detects endogenous levels of total RPS6KC1.
RRID:
AB_2835790
Cite Format: Affinity Biosciences Cat# DF3408, RRID:AB_2835790.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

52 kDa ribosomal protein S6 kinase; humS6PKh1; KS6C1_HUMAN; Ribosomal protein S6 kinase 52kD polypeptide 1; Ribosomal protein S6 kinase 52kDa polypeptide 1; Ribosomal protein S6 kinase C1; Ribosomal protein S6 kinase delta 1; Ribosomal protein S6 kinase delta-1; Ribosomal S6 kinase like protein with two PSK domains 118 kDa protein; Ribosomal S6 kinase-like protein with two PSK domains 118 kDa protein; RPK 118; RPK118; RPS6KC1; RSKL1; S6K-delta-1; S6PKh1; SPHK1 binding protein; SPHK1-binding protein;

Immunogens

Immunogen:
Uniprot:

Q96S38(KS6C1_HUMAN) >>Visit HPA database.

Gene(ID):
RPS6KC1(26750)
Expression:
Q96S38 KS6C1_HUMAN:

Highly expressed in testis, skeletal muscle, brain, heart, placenta, kidney and liver and weakly expressed in thymus, small intestine, lung and colon.

Sequence:
MTSYRERSADLARFYTVTEPQRHPRGYTVYKVTARVVSRRNPEDVQEIIVWKRYSDFKKLHKELWQIHKNLFRHSELFPPFAKGIVFGRFDETVIEERRQCAEDLLQFSANIPALYNSKQLEDFFKGGIINDSSELIGPAEAHSDSLIDTFPECSTEGFSSDSDLVSLTVDVDSLAELDDGMASNQNSPIRTFGLNLSSDSSALGAVASDSEQSKTEEERESRSLFPGSLKPKLGKRDYLEKAGELIKLALKKEEEDDYEAASDFYRKGVDLLLEGVQGESSPTRREAVKRRTAEYLMRAESISSLYGKPQLDDVSQPPGSLSSRPLWNLRSPAEELKAFRVLGVIDKVLLVMDTRTEQTFILKGLRKSSEYSRNRKTIIPRCVPNMVCLHKYIISEESVFLVLQHAEGGKLWSYISKFLNRSPEESFDIKEVKKPTLAKVHLQQPTSSPQDSSSFESRGSDGGSMLKALPLKSSLTPSSQDDSNQEDDGQDSSPKWPDSGSSSEEECTTSYLTLCNEYGQEKIEPGSLNEEPFMKTEGNGVDTKAIKSFPAHLAADSDSPSTQLRAHELKFFPNDDPEAVSSPRTSDSLSRSKNSPMEFFRIDSKDSASELLGLDFGEKLYSLKSEPLKPFFTLPDGDSASRSFNTSESKVEFKAQDTISRGSDDSVPVISFKDAAFDDVSGTDEGRPDLLVNLPGELESTREAAAMGPTKFTQTNIGIIENKLLEAPDVLCLRLSTEQCQAHEEKGIEELSDPSGPKSYSITEKHYAQEDPRMLFVAAVDHSSSGDMSLLPSSDPKFQGLGVVESAVTANNTEESLFRICSPLSGANEYIASTDTLKTEEVLLFTDQTDDLAKEEPTSLFQRDSETKGESGLVLEGDKEIHQIFEDLDKKLALASRFYIPEGCIQRWAAEMVVALDALHREGIVCRDLNPNNILLNDRGHIQLTYFSRWSEVEDSCDSDAIERMYCAPEVGAITEETEACDWWSLGAVLFELLTGKTLVECHPAGINTHTTLNMPECVSEEARSLIQQLLQFNPLERLGAGVAGVEDIKSHPFFTPVDWAELMR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
64
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96S38 As Substrate

Site PTM Type Enzyme
T93 Phosphorylation
S109 Phosphorylation
S118 Phosphorylation
S229 Phosphorylation
K248 Ubiquitination
K253 Ubiquitination
Y259 Phosphorylation
S263 Phosphorylation
Y266 Phosphorylation
K268 Ubiquitination
S281 Phosphorylation
S282 Phosphorylation
T284 Phosphorylation
S302 Phosphorylation
S332 Phosphorylation
T378 Phosphorylation
S423 Phosphorylation
S427 Phosphorylation
K431 Ubiquitination
T437 Phosphorylation
S449 Phosphorylation
S455 Phosphorylation
S461 Phosphorylation
S500 Phosphorylation
S528 Phosphorylation
S549 Phosphorylation
S560 Phosphorylation
K571 Ubiquitination
S582 Phosphorylation
S583 Phosphorylation
S593 Phosphorylation
S596 Phosphorylation
S605 Phosphorylation
K606 Ubiquitination
S608 Phosphorylation
S610 Phosphorylation
K620 Ubiquitination
Y622 Phosphorylation
K630 Ubiquitination
S640 Phosphorylation
S644 Phosphorylation
S648 Phosphorylation
K651 Ubiquitination
T659 Phosphorylation
S661 Phosphorylation
S664 Phosphorylation
S667 Phosphorylation
K674 Ubiquitination
S682 Phosphorylation
K759 Ubiquitination
S760 Phosphorylation
S762 Phosphorylation
K766 Ubiquitination
S784 Phosphorylation
S823 Phosphorylation
S826 Phosphorylation
S834 Phosphorylation
K855 Ubiquitination
S866 Phosphorylation
K869 Ubiquitination
S872 Phosphorylation
K891 Ubiquitination

Research Backgrounds

Function:

May be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell. Plays a role in the recruitment of PRDX3 to early endosomes.

Subcellular Location:

Cytoplasm. Membrane. Early endosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis, skeletal muscle, brain, heart, placenta, kidney and liver and weakly expressed in thymus, small intestine, lung and colon.

Subunit Structure:

Interacts with SPHK1 and phosphatidylinositol 3-phosphate. Interacts (via PX domain) with PRDX3.

Family&Domains:

The PX domain is essential for its localization to the early endosomes.

The first protein kinase domain appears to be a pseudokinase domain as it does not contain the classical characteristics, such as the ATP-binding motif, ATP-binding site and active site.

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. S6 kinase subfamily.

Restrictive clause

 

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