Product: MOS Antibody
Catalog: AF0355
Description: Rabbit polyclonal antibody to MOS
Application: WB IF/ICC
Reactivity: Human, Monkey
Prediction: Pig, Zebrafish, Bovine, Dog, Chicken, Xenopus
Mol.Wt.: 40kDa; 38kD(Calculated).
Uniprot: P00540
RRID: AB_2833521

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Monkey
Prediction:
Pig(91%), Zebrafish(88%), Bovine(82%), Dog(91%), Chicken(91%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
MOS Antibody detects endogenous levels of total MOS.
RRID:
AB_2833521
Cite Format: Affinity Biosciences Cat# AF0355, RRID:AB_2833521.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

c mos; MGC119962; MGC119963; MOS; MOS_HUMAN; MSV; Oocyte maturation factor mos; Proto oncogene serine / threonine protein kinase mos; Proto-oncogene c-Mos; Proto-oncogene serine/threonine-protein kinase mos; v mos Moloney murine sarcoma viral oncogene homolog;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P00540 MOS_HUMAN:

Expressed specifically in testis during spermatogenesis.

Description:
Mos a proto-oncogenic serine/threonine kinase of the MOS family. Expressed predominantly in germ cells. Crucial for normal oocyte meiosis and female fertility in mice.
Sequence:
MPSPLALRPYLRSEFSPSVDARPCSSPSELPAKLLLGATLPRAPRLPRRLAWCSIDWEQVCLLQRLGAGGFGSVYKATYRGVPVAIKQVNKCTKNRLASRRSFWAELNVARLRHDNIVRVVAASTRTPAGSNSLGTIIMEFGGNVTLHQVIYGAAGHPEGDAGEPHCRTGGQLSLGKCLKYSLDVVNGLLFLHSQSIVHLDLKPANILISEQDVCKISDFGCSEKLEDLLCFQTPSYPLGGTYTHRAPELLKGEGVTPKADIYSFAITLWQMTTKQAPYSGERQHILYAVVAYDLRPSLSAAVFEDSLPGQRLGDVIQRCWRPSAAQRPSARLLLVDLTSLKAELG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Dog
91
Chicken
91
Zebrafish
88
Bovine
82
Xenopus
82
Horse
0
Sheep
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00540 As Substrate

Site PTM Type Enzyme
S3 Phosphorylation
S16 Phosphorylation
Y263 Phosphorylation
T339 Phosphorylation
S340 Phosphorylation

PTMs - P00540 As Enzyme

Substrate Site Source
P08670 (VIM) T458 Uniprot
P08670 (VIM) S459 Uniprot
Q02750 (MAP2K1) S218 Uniprot
Q02750 (MAP2K1) S222 Uniprot

Research Backgrounds

Tissue Specificity:

Expressed specifically in testis during spermatogenesis.

Family&Domains:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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